Antibodies against collagen types III and VI have been localized by electron immunohistochemistry with two different techniques in normal rat uterus and kidney. Antibodies directed against two components of the extracellular matrix with known localization, laminin and type IV collagen, were used as controls for the specificity of the localization. The results demonstrate that types III and VI are found in the interstitium as fine (10- to 15-nm), beaded fibrils and filaments (6- to 10-nm), respectively. Both are often found associated with thick, crossbanded type I collagen fibers (30- to 35-nm) and occasionally associated with some basement membranes adjacent to the interstitium. Further, the findings suggest that collagens III and VI may connect the various components of the extracellular matrix, such as type I fibers with basement membranes and other structures, thus forming an integrated functional unit.