Calcium alginate template-mineral substituted hydroxyapatite hydrogel coated titanium implant for tibia bone regeneration. 2020

XinHua Yin, and Liang Yan, and Ding Jun Hao, and Shichang Liu, and Ming Yang, and BaoRong He, and ZhongKai Liu
Department of Spine Surgery, Hong Hui Hospital, Xi'an Jiaotong University College of Medicine, No. 76 Nanguo Road, Beilin District, Xi'an, Shaanxi Province 710054, China.

Osteogenic differentiation is great significance for improving the bone regeneration. Present study evaluates the osteogenic ability of lanthanum (La3+) and silicate (SiO44-) substituted hydroxyapatite (MHAP) - polymeric composite coated surface treated titanium (Ti) implant. The bio-ceramic MHAP was synthesized by hydrothermal process with assistance of calcium alginate template. For enhance the hydrophilicity, the polymer poly (vinyl pyrrolidone) (PVP) was included in the composite by ultra-sonication method. The negative zeta potential value -9.97 mV of Ca-alg/ La, Si-HAP was observed after the incorporation of PVP in the matrix. Incorporation of minerals and PVP polymer was confirmed and analyzed by Energy Dispersive X-ray analysis (EDX), Fourier Transform Infra-Red spectroscopy (FT-IR) and Electron Microscopy techniques. A compact coating of the composite with the thickness of 448 nm on Ti surface was achieved by Electrophoretic deposition (EPD) method. The in-vitro MTT assay method and alkaline phosphate ALP activity (94% and 0.94 a.u respectively for the optimized composite) were utilized to determine the cell viability and differentiation on human Bone Marrow-Derived Stem Cells (hBMSCs). The osteogenic ability of bio-composite coated Ti in hBMSCs and in-vivo rat model has strongly suggests the fabricated Ti plate with bio-composite coatings can act as promising biomaterial for orthopedics.

UI MeSH Term Description Entries
D007811 Lanthanum The prototypical element in the rare earth family of metals. It has the atomic symbol La, atomic number 57, and atomic weight 138.91. Lanthanide ion is used in experimental biology as a calcium antagonist; lanthanum oxide improves the optical properties of glass.
D011474 Prosthesis Design The plan and delineation of prostheses in general or a specific prosthesis. Design, Prosthesis,Designs, Prosthesis,Prosthesis Designs
D002478 Cells, Cultured Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others. Cultured Cells,Cell, Cultured,Cultured Cell
D004339 Drug Compounding The preparation, mixing, and assembly of a drug. (From Remington, The Science and Practice of Pharmacy, 19th ed, p1814). Drug Formulation,Drug Preparation,Drug Microencapsulation,Pharmaceutical Formulation,Compounding, Drug,Formulation, Drug,Formulation, Pharmaceutical,Microencapsulation, Drug,Preparation, Drug
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D006882 Hydroxyapatites A group of compounds with the general formula M10(PO4)6(OH)2, where M is barium, strontium, or calcium. The compounds are the principal mineral in phosphorite deposits, biological tissue, human bones, and teeth. They are also used as an anticaking agent and polymer catalysts. (Grant & Hackh's Chemical Dictionary, 5th ed) Hydroxyapatite Derivatives,Derivatives, Hydroxyapatite
D000069343 Bone-Implant Interface Region of interaction between the bone and PROSTHESES AND IMPLANTS which becomes bonded through cellular and extracellular interactions similar to OSSEOINTEGRATION. Bone-Prosthesis Interface,Bone Implant Interface,Bone Prosthesis Interface,Bone-Implant Interfaces,Bone-Prosthesis Interfaces,Interface, Bone-Implant,Interface, Bone-Prosthesis,Interfaces, Bone-Implant,Interfaces, Bone-Prosthesis
D000077280 Bone-Anchored Prosthesis A prosthesis designed to attach directly to the bone via fixtures implanted into the bone rather than a socket. Bone Anchored Devices,Bone-Anchored Amputation Prosthesis,Bone-Anchored Implants,Intraosseous Transcutaneous Amputation Prosthesis,Osseo-Anchored Prosthesis,Osseo-Integrated Implants,Osseointegrated Implants,Amputation Prostheses, Bone-Anchored,Amputation Prosthesis, Bone-Anchored,Bone Anchored Amputation Prosthesis,Bone Anchored Device,Bone Anchored Implants,Bone Anchored Prosthesis,Bone-Anchored Amputation Prostheses,Bone-Anchored Implant,Bone-Anchored Prostheses,Device, Bone Anchored,Implant, Bone-Anchored,Implant, Osseo-Integrated,Implant, Osseointegrated,Osseo Anchored Prosthesis,Osseo Integrated Implants,Osseo-Anchored Prostheses,Osseo-Integrated Implant,Osseointegrated Implant,Prostheses, Bone-Anchored,Prostheses, Osseo-Anchored,Prosthesis, Bone-Anchored,Prosthesis, Bone-Anchored Amputation,Prosthesis, Osseo-Anchored
D000464 Alginates Salts and esters of ALGINIC ACID that are used as HYDROGELS; DENTAL IMPRESSION MATERIALS, and as absorbent materials for surgical dressings (BANDAGES, HYDROCOLLOID). They are also used to manufacture MICROSPHERES and NANOPARTICLES for DIAGNOSTIC REAGENT KITS and DRUG DELIVERY SYSTEMS. Alginate,Alginic Acid, Barium Salt,Alginic Acid, Calcium Salt,Alginic Acid, Copper Salt,Alginic Acid, Potassium Salt,Alginic Acid, Sodium Salt,Alloid G,Barium Alginate,Calcium Alginate,Calginat,Copper Alginate,Kalrostat,Kalrostat 2,Kaltostat,Potassium Alginate,Sodium Alginate,Sodium Calcium Alginate,Vocoloid,Xantalgin,poly(Mannuronic Acid), Sodium Salt,Alginate, Barium,Alginate, Calcium,Alginate, Copper,Alginate, Potassium,Alginate, Sodium,Alginate, Sodium Calcium,Calcium Alginate, Sodium
D000469 Alkaline Phosphatase An enzyme that catalyzes the conversion of an orthophosphoric monoester and water to an alcohol and orthophosphate. EC 3.1.3.1.

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