-transparent.png){kind=logo}
Ribosome-associated vesicles: A dynamic subcompartment of the endoplasmic reticulum in secretory cells. 2020
Stephen D Carter, and
Cheri M Hampton, and
Robert Langlois, and
Roberto Melero, and
Zachary J Farino, and
Michael J Calderon, and
Wen Li, and
Callen T Wallace, and
Ngoc Han Tran, and
Robert A Grassucci, and
Stephanie E Siegmund, and
Joshua Pemberton, and
Travis J Morgenstern, and
Leanna Eisenman, and
Jenny I Aguilar, and
Nili L Greenberg, and
Elana S Levy, and
Edward Yi, and
William G Mitchell, and
William J Rice, and
Christoph Wigge, and
Jyotsna Pilli, and
Emily W George, and
Despoina Aslanoglou, and
Maïté Courel, and
Robin J Freyberg, and
Jonathan A Javitch, and
Zachary P Wills, and
Estela Area-Gomez, and
Sruti Shiva, and
Francesca Bartolini, and
Allen Volchuk, and
Sandra A Murray, and
Meir Aridor, and
Kenneth N Fish, and
Peter Walter, and
Tamas Balla, and
Deborah Fass, and
Sharon G Wolf, and
Simon C Watkins, and
José María Carazo, and
Grant J Jensen, and
Joachim Frank, and
Zachary Freyberg
Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA 91125, USA.
The endoplasmic reticulum (ER) is a highly dynamic network of membranes. Here, we combine live-cell microscopy with in situ cryo-electron tomography to directly visualize ER dynamics in several secretory cell types including pancreatic β-cells and neurons under near-native conditions. Using these imaging approaches, we identify a novel, mobile form of ER, ribosome-associated vesicles (RAVs), found primarily in the cell periphery, which is conserved across different cell types and species. We show that RAVs exist as distinct, highly dynamic structures separate from the intact ER reticular architecture that interact with mitochondria via direct intermembrane contacts. These findings describe a new ER subcompartment within cells.
Related Publications
Stephen D Carter, and
Cheri M Hampton, and
Robert Langlois, and
Roberto Melero, and
Zachary J Farino, and
Michael J Calderon, and
Wen Li, and
Callen T Wallace, and
Ngoc Han Tran, and
Robert A Grassucci, and
Stephanie E Siegmund, and
Joshua Pemberton, and
Travis J Morgenstern, and
Leanna Eisenman, and
Jenny I Aguilar, and
Nili L Greenberg, and
Elana S Levy, and
Edward Yi, and
William G Mitchell, and
William J Rice, and
Christoph Wigge, and
Jyotsna Pilli, and
Emily W George, and
Despoina Aslanoglou, and
Maïté Courel, and
Robin J Freyberg, and
Jonathan A Javitch, and
Zachary P Wills, and
Estela Area-Gomez, and
Sruti Shiva, and
Francesca Bartolini, and
Allen Volchuk, and
Sandra A Murray, and
Meir Aridor, and
Kenneth N Fish, and
Peter Walter, and
Tamas Balla, and
Deborah Fass, and
Sharon G Wolf, and
Simon C Watkins, and
José María Carazo, and
Grant J Jensen, and
Joachim Frank, and
Zachary Freyberg
December 1993,
Proceedings of the National Academy of Sciences of the United States of America,
Stephen D Carter, and
Cheri M Hampton, and
Robert Langlois, and
Roberto Melero, and
Zachary J Farino, and
Michael J Calderon, and
Wen Li, and
Callen T Wallace, and
Ngoc Han Tran, and
Robert A Grassucci, and
Stephanie E Siegmund, and
Joshua Pemberton, and
Travis J Morgenstern, and
Leanna Eisenman, and
Jenny I Aguilar, and
Nili L Greenberg, and
Elana S Levy, and
Edward Yi, and
William G Mitchell, and
William J Rice, and
Christoph Wigge, and
Jyotsna Pilli, and
Emily W George, and
Despoina Aslanoglou, and
Maïté Courel, and
Robin J Freyberg, and
Jonathan A Javitch, and
Zachary P Wills, and
Estela Area-Gomez, and
Sruti Shiva, and
Francesca Bartolini, and
Allen Volchuk, and
Sandra A Murray, and
Meir Aridor, and
Kenneth N Fish, and
Peter Walter, and
Tamas Balla, and
Deborah Fass, and
Sharon G Wolf, and
Simon C Watkins, and
José María Carazo, and
Grant J Jensen, and
Joachim Frank, and
Zachary Freyberg
February 2004,
Molecular biology of the cell,
Stephen D Carter, and
Cheri M Hampton, and
Robert Langlois, and
Roberto Melero, and
Zachary J Farino, and
Michael J Calderon, and
Wen Li, and
Callen T Wallace, and
Ngoc Han Tran, and
Robert A Grassucci, and
Stephanie E Siegmund, and
Joshua Pemberton, and
Travis J Morgenstern, and
Leanna Eisenman, and
Jenny I Aguilar, and
Nili L Greenberg, and
Elana S Levy, and
Edward Yi, and
William G Mitchell, and
William J Rice, and
Christoph Wigge, and
Jyotsna Pilli, and
Emily W George, and
Despoina Aslanoglou, and
Maïté Courel, and
Robin J Freyberg, and
Jonathan A Javitch, and
Zachary P Wills, and
Estela Area-Gomez, and
Sruti Shiva, and
Francesca Bartolini, and
Allen Volchuk, and
Sandra A Murray, and
Meir Aridor, and
Kenneth N Fish, and
Peter Walter, and
Tamas Balla, and
Deborah Fass, and
Sharon G Wolf, and
Simon C Watkins, and
José María Carazo, and
Grant J Jensen, and
Joachim Frank, and
Zachary Freyberg
January 1998,
Cell motility and the cytoskeleton,
Stephen D Carter, and
Cheri M Hampton, and
Robert Langlois, and
Roberto Melero, and
Zachary J Farino, and
Michael J Calderon, and
Wen Li, and
Callen T Wallace, and
Ngoc Han Tran, and
Robert A Grassucci, and
Stephanie E Siegmund, and
Joshua Pemberton, and
Travis J Morgenstern, and
Leanna Eisenman, and
Jenny I Aguilar, and
Nili L Greenberg, and
Elana S Levy, and
Edward Yi, and
William G Mitchell, and
William J Rice, and
Christoph Wigge, and
Jyotsna Pilli, and
Emily W George, and
Despoina Aslanoglou, and
Maïté Courel, and
Robin J Freyberg, and
Jonathan A Javitch, and
Zachary P Wills, and
Estela Area-Gomez, and
Sruti Shiva, and
Francesca Bartolini, and
Allen Volchuk, and
Sandra A Murray, and
Meir Aridor, and
Kenneth N Fish, and
Peter Walter, and
Tamas Balla, and
Deborah Fass, and
Sharon G Wolf, and
Simon C Watkins, and
José María Carazo, and
Grant J Jensen, and
Joachim Frank, and
Zachary Freyberg
July 1986,
The Journal of cell biology,
Stephen D Carter, and
Cheri M Hampton, and
Robert Langlois, and
Roberto Melero, and
Zachary J Farino, and
Michael J Calderon, and
Wen Li, and
Callen T Wallace, and
Ngoc Han Tran, and
Robert A Grassucci, and
Stephanie E Siegmund, and
Joshua Pemberton, and
Travis J Morgenstern, and
Leanna Eisenman, and
Jenny I Aguilar, and
Nili L Greenberg, and
Elana S Levy, and
Edward Yi, and
William G Mitchell, and
William J Rice, and
Christoph Wigge, and
Jyotsna Pilli, and
Emily W George, and
Despoina Aslanoglou, and
Maïté Courel, and
Robin J Freyberg, and
Jonathan A Javitch, and
Zachary P Wills, and
Estela Area-Gomez, and
Sruti Shiva, and
Francesca Bartolini, and
Allen Volchuk, and
Sandra A Murray, and
Meir Aridor, and
Kenneth N Fish, and
Peter Walter, and
Tamas Balla, and
Deborah Fass, and
Sharon G Wolf, and
Simon C Watkins, and
José María Carazo, and
Grant J Jensen, and
Joachim Frank, and
Zachary Freyberg
May 1999,
The Journal of biological chemistry,
Stephen D Carter, and
Cheri M Hampton, and
Robert Langlois, and
Roberto Melero, and
Zachary J Farino, and
Michael J Calderon, and
Wen Li, and
Callen T Wallace, and
Ngoc Han Tran, and
Robert A Grassucci, and
Stephanie E Siegmund, and
Joshua Pemberton, and
Travis J Morgenstern, and
Leanna Eisenman, and
Jenny I Aguilar, and
Nili L Greenberg, and
Elana S Levy, and
Edward Yi, and
William G Mitchell, and
William J Rice, and
Christoph Wigge, and
Jyotsna Pilli, and
Emily W George, and
Despoina Aslanoglou, and
Maïté Courel, and
Robin J Freyberg, and
Jonathan A Javitch, and
Zachary P Wills, and
Estela Area-Gomez, and
Sruti Shiva, and
Francesca Bartolini, and
Allen Volchuk, and
Sandra A Murray, and
Meir Aridor, and
Kenneth N Fish, and
Peter Walter, and
Tamas Balla, and
Deborah Fass, and
Sharon G Wolf, and
Simon C Watkins, and
José María Carazo, and
Grant J Jensen, and
Joachim Frank, and
Zachary Freyberg
January 1991,
Cell biophysics,
Stephen D Carter, and
Cheri M Hampton, and
Robert Langlois, and
Roberto Melero, and
Zachary J Farino, and
Michael J Calderon, and
Wen Li, and
Callen T Wallace, and
Ngoc Han Tran, and
Robert A Grassucci, and
Stephanie E Siegmund, and
Joshua Pemberton, and
Travis J Morgenstern, and
Leanna Eisenman, and
Jenny I Aguilar, and
Nili L Greenberg, and
Elana S Levy, and
Edward Yi, and
William G Mitchell, and
William J Rice, and
Christoph Wigge, and
Jyotsna Pilli, and
Emily W George, and
Despoina Aslanoglou, and
Maïté Courel, and
Robin J Freyberg, and
Jonathan A Javitch, and
Zachary P Wills, and
Estela Area-Gomez, and
Sruti Shiva, and
Francesca Bartolini, and
Allen Volchuk, and
Sandra A Murray, and
Meir Aridor, and
Kenneth N Fish, and
Peter Walter, and
Tamas Balla, and
Deborah Fass, and
Sharon G Wolf, and
Simon C Watkins, and
José María Carazo, and
Grant J Jensen, and
Joachim Frank, and
Zachary Freyberg
June 1996,
The Biochemical journal,
Stephen D Carter, and
Cheri M Hampton, and
Robert Langlois, and
Roberto Melero, and
Zachary J Farino, and
Michael J Calderon, and
Wen Li, and
Callen T Wallace, and
Ngoc Han Tran, and
Robert A Grassucci, and
Stephanie E Siegmund, and
Joshua Pemberton, and
Travis J Morgenstern, and
Leanna Eisenman, and
Jenny I Aguilar, and
Nili L Greenberg, and
Elana S Levy, and
Edward Yi, and
William G Mitchell, and
William J Rice, and
Christoph Wigge, and
Jyotsna Pilli, and
Emily W George, and
Despoina Aslanoglou, and
Maïté Courel, and
Robin J Freyberg, and
Jonathan A Javitch, and
Zachary P Wills, and
Estela Area-Gomez, and
Sruti Shiva, and
Francesca Bartolini, and
Allen Volchuk, and
Sandra A Murray, and
Meir Aridor, and
Kenneth N Fish, and
Peter Walter, and
Tamas Balla, and
Deborah Fass, and
Sharon G Wolf, and
Simon C Watkins, and
José María Carazo, and
Grant J Jensen, and
Joachim Frank, and
Zachary Freyberg
November 2020,
Journal of cell science,
Stephen D Carter, and
Cheri M Hampton, and
Robert Langlois, and
Roberto Melero, and
Zachary J Farino, and
Michael J Calderon, and
Wen Li, and
Callen T Wallace, and
Ngoc Han Tran, and
Robert A Grassucci, and
Stephanie E Siegmund, and
Joshua Pemberton, and
Travis J Morgenstern, and
Leanna Eisenman, and
Jenny I Aguilar, and
Nili L Greenberg, and
Elana S Levy, and
Edward Yi, and
William G Mitchell, and
William J Rice, and
Christoph Wigge, and
Jyotsna Pilli, and
Emily W George, and
Despoina Aslanoglou, and
Maïté Courel, and
Robin J Freyberg, and
Jonathan A Javitch, and
Zachary P Wills, and
Estela Area-Gomez, and
Sruti Shiva, and
Francesca Bartolini, and
Allen Volchuk, and
Sandra A Murray, and
Meir Aridor, and
Kenneth N Fish, and
Peter Walter, and
Tamas Balla, and
Deborah Fass, and
Sharon G Wolf, and
Simon C Watkins, and
José María Carazo, and
Grant J Jensen, and
Joachim Frank, and
Zachary Freyberg
July 1988,
Cell,
Stephen D Carter, and
Cheri M Hampton, and
Robert Langlois, and
Roberto Melero, and
Zachary J Farino, and
Michael J Calderon, and
Wen Li, and
Callen T Wallace, and
Ngoc Han Tran, and
Robert A Grassucci, and
Stephanie E Siegmund, and
Joshua Pemberton, and
Travis J Morgenstern, and
Leanna Eisenman, and
Jenny I Aguilar, and
Nili L Greenberg, and
Elana S Levy, and
Edward Yi, and
William G Mitchell, and
William J Rice, and
Christoph Wigge, and
Jyotsna Pilli, and
Emily W George, and
Despoina Aslanoglou, and
Maïté Courel, and
Robin J Freyberg, and
Jonathan A Javitch, and
Zachary P Wills, and
Estela Area-Gomez, and
Sruti Shiva, and
Francesca Bartolini, and
Allen Volchuk, and
Sandra A Murray, and
Meir Aridor, and
Kenneth N Fish, and
Peter Walter, and
Tamas Balla, and
Deborah Fass, and
Sharon G Wolf, and
Simon C Watkins, and
José María Carazo, and
Grant J Jensen, and
Joachim Frank, and
Zachary Freyberg
March 1996,
The Journal of biological chemistry,
