Molecular Detection of Anaplasma marginale and Anaplasma ovis (Rickettsiales: Anaplasmataceae) in Ixodid Tick Species in Iran. 2020

A Hosseini-Chegeni, and M Tavakoli, and Gh Goudarzi, and Z Telmadarraiy, and M Sharifdini, and F Faghihi, and M K Ghanbari
Department of Plant Protection, Faculty of Agriculture, Lorestan University, Khorramabad, Iran.

The present study was conducted as the first molecular detection of Anaplasma species in tick samples based on the sequencing of major surface proteins 4 (msp4) gene fragments in different parts of Iran. A total of 130 tick specimens were collected from Hormozgan, Lorestan, and Guilan, Iran, within 2015 to 2017. Hyalomma asiaticum, Hyalomma dromedarii, Rhipicephalus sanguineus, and Rhipicephalus (Boophilus) species were identified in different geographical regions. An amplicon of 464-bp msp4 of Anaplasma was amplified using polymerase chain reaction in various tick species. Three sequences, including one Anaplasma marginale from R. (Boophilus) species and two Anaplasma ovis from Rhipicephalus sanguineus, were obtained after sequencing. It is concluded that bovine and ovine anaplasmosis agents are present in tick samples in Iran. The use of the gene families of six major surface proteins for the detection of various Anaplasma species is recommended.

UI MeSH Term Description Entries
D007492 Iran A country bordering the Gulf of Oman, the Persian Gulf, and the Caspian Sea, between Iraq and Pakistan. The capital is Tehran. Islamic Republic of Iran
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D016133 Polymerase Chain Reaction In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships. Anchored PCR,Inverse PCR,Nested PCR,PCR,Anchored Polymerase Chain Reaction,Inverse Polymerase Chain Reaction,Nested Polymerase Chain Reaction,PCR, Anchored,PCR, Inverse,PCR, Nested,Polymerase Chain Reactions,Reaction, Polymerase Chain,Reactions, Polymerase Chain
D042322 Anaplasma marginale A species of gram-negative bacteria and causative agent of severe bovine ANAPLASMOSIS. It is the most pathogenic of the ANAPLASMA species. Anaplasma argentium,Anaplasma rossicum,Anaplasma theileri
D042323 Anaplasma ovis A species of gram-negative bacteria producing mild to severe ANAPLASMOSIS in SHEEP and GOATS, and mild or inapparent infections in DEER and CATTLE.
D048494 Rhipicephalus A genus of TICKS, in the family IXODIDAE, widespread in Africa. Members of the genus include many important vectors of animal and human pathogens.
D048508 Rhipicephalus sanguineus A species of tick (TICKS) in the family IXODIDAE, distributed throughout the world but abundant in southern Europe. It will feed on a wide variety of MAMMALS, but DOGS are its preferred host. It transmits a large number of diseases including BABESIOSIS; THEILERIASIS; EHRLICHIOSIS; and MEDITERRANEAN SPOTTED FEVER. Brown Dog Tick,Brown Dog Ticks,Dog Tick, Brown,Dog Ticks, Brown,Tick, Brown Dog,Ticks, Brown Dog
D026863 Ixodidae A family of hardbacked TICKS, in the subclass ACARI. Genera include DERMACENTOR and IXODES among others. Ticks, Hardbacked,Hard Ticks,Hard Tick,Hardbacked Tick,Hardbacked Ticks,Tick, Hard,Tick, Hardbacked,Ticks, Hard

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