-transparent.png){kind=logo}
Acrylamide was found to be an effective quencher of Yt-base (Y-4,9-dihydro-4,6-dimethyl-9-oxo-1H-imidazo-1,2a-purine) in water. In the absence of collisional quenching the decay of Yt-base in water is predominantly a single exponential. The intensity decays become increasingly heterogeneous when quenched by acrylamide. The frequency-domain data were analyzed using the radiation model, which provides estimates of molecular parameters characteristic of the system. The mutual diffusion coefficient at 20 degrees C was found to be 0.5 x 10(5) cm2/s, the Yt-base acrylamide interaction radius was 8 A, and the rate constant for quenching was 100 cm/s. These values indicate that quenching is diffusion-limited, i.e., the encounter complex is deactivated at least 2-fold faster than the rate of diffusive encounters.
I Gryczynski, and
M L Johnson, and
J R Lakowicz
January 1979,
Nucleic acids research,
I Gryczynski, and
M L Johnson, and
J R Lakowicz
January 1997,
Biopolymers,
I Gryczynski, and
M L Johnson, and
J R Lakowicz
March 1948,
The Journal of physical and colloid chemistry,
I Gryczynski, and
M L Johnson, and
J R Lakowicz
December 2014,
Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy,
I Gryczynski, and
M L Johnson, and
J R Lakowicz
March 2008,
Plasmonics (Norwell, Mass.),
I Gryczynski, and
M L Johnson, and
J R Lakowicz
January 1982,
Biochemistry,
I Gryczynski, and
M L Johnson, and
J R Lakowicz
September 1983,
Photochemistry and photobiology,
I Gryczynski, and
M L Johnson, and
J R Lakowicz
May 2014,
Journal of fluorescence,
I Gryczynski, and
M L Johnson, and
J R Lakowicz
December 1980,
FEBS letters,
I Gryczynski, and
M L Johnson, and
J R Lakowicz
September 2014,
Physical chemistry chemical physics : PCCP,
