Biomaterial Database

Sodium-dependent L-lactate uptake by bovine intestinal brush border membrane vesicles. 1988

S Wolffram, and B Grenacher, and E Scharrer

Institut für Veterinär-Physiologie, Universität Zürich, Switzerland.

In ruminants, intestinal digesta can contain considerable amounts of lactic acid derived from ingestion of lactic acid-containing feed and from production of lactic acid during ruminal digestion of readily fermentable carbohydrates. The aim of the present study was to investigate L-lactate transport across the bovine intestinal brush border membrane. The experiments were performed using isolated brush border membrane vesicles from the midjejunum of heifers. The results demonstrate the existence of Na+-stimulated L-lactate uptake by the brush border membrane vesicles. Acetate, propionate, and butyrate strongly inhibited Na+-dependent L-lactate transport. Acetate caused a 58% inhibition, whereas propionate and butyrate completely inhibited Na+-dependent uptake. Kinetic evaluation of L-lactate uptake in the presence or absence of extravesicular butyrate suggests a competitive inhibition by butyrate. Among the phenolic acids tested in this study only trans-cinnamic acid caused a significant reduction of L-lactate uptake, whereas cumaric acid, ferulic acid, and gallic acid only slightly reduced L-lactate transport. Thus, the L-lactate transporter appears to have some affinity for transcinnamic acid.

UI	MeSH Term	Description	Entries
D007408	Intestinal Absorption	Uptake of substances through the lining of the INTESTINES.	Absorption, Intestinal
D007583	Jejunum	The middle portion of the SMALL INTESTINE, between DUODENUM and ILEUM. It represents about 2/5 of the remaining portion of the small intestine below duodenum.	Jejunums
D007773	Lactates	Salts or esters of LACTIC ACID containing the general formula CH3CHOHCOOR.
D008871	Microvilli	Minute projections of cell membranes which greatly increase the surface area of the cell.	Brush Border,Striated Border,Border, Brush,Border, Striated,Borders, Brush,Borders, Striated,Brush Borders,Microvillus,Striated Borders
D002417	Cattle	Domesticated bovine animals of the genus Bos, usually kept on a farm or ranch and used for the production of meat or dairy products or for heavy labor.	Beef Cow,Bos grunniens,Bos indicus,Bos indicus Cattle,Bos taurus,Cow,Cow, Domestic,Dairy Cow,Holstein Cow,Indicine Cattle,Taurine Cattle,Taurus Cattle,Yak,Zebu,Beef Cows,Bos indicus Cattles,Cattle, Bos indicus,Cattle, Indicine,Cattle, Taurine,Cattle, Taurus,Cattles, Bos indicus,Cattles, Indicine,Cattles, Taurine,Cattles, Taurus,Cow, Beef,Cow, Dairy,Cow, Holstein,Cows,Dairy Cows,Domestic Cow,Domestic Cows,Indicine Cattles,Taurine Cattles,Taurus Cattles,Yaks,Zebus
D005260	Female		Females
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D001692	Biological Transport	The movement of materials (including biochemical substances and drugs) through a biological system at the cellular level. The transport can be across cell membranes and epithelial layers. It also can occur within intracellular compartments and extracellular compartments.	Transport, Biological,Biologic Transport,Transport, Biologic
D012964	Sodium	A member of the alkali group of metals. It has the atomic symbol Na, atomic number 11, and atomic weight 23.	Sodium Ion Level,Sodium-23,Ion Level, Sodium,Level, Sodium Ion,Sodium 23
D066298	In Vitro Techniques	Methods to study reactions or processes taking place in an artificial environment outside the living organism.	In Vitro Test,In Vitro Testing,In Vitro Tests,In Vitro as Topic,In Vitro,In Vitro Technique,In Vitro Testings,Technique, In Vitro,Techniques, In Vitro,Test, In Vitro,Testing, In Vitro,Testings, In Vitro,Tests, In Vitro,Vitro Testing, In