Biomaterial Database

Plasma cholinesterase genetic variants phenotyped using a Cobas-Fara centrifugal analyser. 1988

A Brock

Department of Clinical Chemistry, University of Aarhus, Denmark.

A two-step colorimetric method that overcomes the difficulties of the classic 240 nm benzoylcholine assay for plasma cholinesterase has been adapted to a Cobas-Fara centrifugal analyser, using choline oxidase coupled with peroxidase/phenol/aminoantipyrine for detection of the choline produced. Reaction conditions of the main reaction are identical to those of the classical benzoylcholine assay. The described method was applied to 105 selected serum samples, previously classified by the Danish Cholinesterase Research Unit as homo- or heterozygous for the usual (U), atypical (A), fluoride-resistant (F), or silent (S) allelic variants. The method showed a distinct separation of the various phenotypes, with catalytic activity concentrations, dibucaine numbers, and fluoride numbers directly comparable to established reference values of the manual 240 nm benzoylcholine method.

UI	MeSH Term	Description	Entries
D007202	Indicators and Reagents	Substances used for the detection, identification, analysis, etc. of chemical, biological, or pathologic processes or conditions. Indicators are substances that change in physical appearance, e.g., color, at or approaching the endpoint of a chemical titration, e.g., on the passage between acidity and alkalinity. Reagents are substances used for the detection or determination of another substance by chemical or microscopical means, especially analysis. Types of reagents are precipitants, solvents, oxidizers, reducers, fluxes, and colorimetric reagents. (From Grant & Hackh's Chemical Dictionary, 5th ed, p301, p499)	Indicator,Reagent,Reagents,Indicators,Reagents and Indicators
D010641	Phenotype	The outward appearance of the individual. It is the product of interactions between genes, and between the GENOTYPE and the environment.	Phenotypes
D002091	Butyrylcholinesterase	An aspect of cholinesterase (EC 3.1.1.8).	Pseudocholinesterase,Benzoylcholinesterase,Butyrylthiocholinesterase
D002498	Centrifugation	Process of using a rotating machine to generate centrifugal force to separate substances of different densities, remove moisture, or simulate gravitational effects. It employs a large motor-driven apparatus with a long arm, at the end of which human and animal subjects, biological specimens, or equipment can be revolved and rotated at various speeds to study gravitational effects. (From Websters, 10th ed; McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
D002802	Cholinesterases		Acylcholineacylhydrolase,Cholase,Cholinesterase
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D014644	Genetic Variation	Genotypic differences observed among individuals in a population.	Genetic Diversity,Variation, Genetic,Diversity, Genetic,Diversities, Genetic,Genetic Diversities,Genetic Variations,Variations, Genetic