Biomaterial Database

Leakage of sulphobromophthalein from large simple bilayer phospholipid vesicles. 1988

A Catalá, and R Zanneti

Instituto Multidisciplinario de Biología Celular, IMBICE CC 403, La Plata, Argentina.

Single bilayer phospholipid vesicles containing entrapped sulphobromophthalein were prepared from a phospholipid film in the presence of deoxicholate at a molar ratio of deoxicholate to phospholipid of 1:2. The trapping efficiency of these vesicles was a function of sulphobromophthalein concentration during incubation, reaching a maximum at a molar ratio of egg lecithin to sulphobromophthalein of 25:1. Release of sulphobromophthalein from liposomes was observed when the vesicles were incubated with bovine serum albumin. The sulphobromophthalein released appeared to be associated with albumin when analysed by Sepharose 4B gel filtration. Leakage of sulphobromophthalein from liposomes was also observed when the vesicles were incubated with different amounts of oleic acid. However, the amount of sulphobromophthalein released from liposomes was much lower than that observed in the presence of bovine serum albumin. These vesicles incorporated up to one molecule of oleic acid for each two and a half molecules of egg lecithin, and appear to be suitable as acceptors in studies of fatty acid transfer between membranes.

UI	MeSH Term	Description	Entries
D008081	Liposomes	Artificial, single or multilaminar vesicles (made from lecithins or other lipids) that are used for the delivery of a variety of biological molecules or molecular complexes to cells, for example, drug delivery and gene transfer. They are also used to study membranes and membrane proteins.	Niosomes,Transferosomes,Ultradeformable Liposomes,Liposomes, Ultra-deformable,Liposome,Liposome, Ultra-deformable,Liposome, Ultradeformable,Liposomes, Ultra deformable,Liposomes, Ultradeformable,Niosome,Transferosome,Ultra-deformable Liposome,Ultra-deformable Liposomes,Ultradeformable Liposome
D008567	Membranes, Artificial	Artificially produced membranes, such as semipermeable membranes used in artificial kidney dialysis (RENAL DIALYSIS), monomolecular and bimolecular membranes used as models to simulate biological CELL MEMBRANES. These membranes are also used in the process of GUIDED TISSUE REGENERATION.	Artificial Membranes,Artificial Membrane,Membrane, Artificial
D009829	Oleic Acids	A group of fatty acids that contain 18 carbon atoms and a double bond at the omega 9 carbon.	Octadecenoic Acids,Acids, Octadecenoic,Acids, Oleic
D010713	Phosphatidylcholines	Derivatives of PHOSPHATIDIC ACIDS in which the phosphoric acid is bound in ester linkage to a CHOLINE moiety.	Choline Phosphoglycerides,Choline Glycerophospholipids,Phosphatidyl Choline,Phosphatidyl Cholines,Phosphatidylcholine,Choline, Phosphatidyl,Cholines, Phosphatidyl,Glycerophospholipids, Choline,Phosphoglycerides, Choline
D010743	Phospholipids	Lipids containing one or more phosphate groups, particularly those derived from either glycerol (phosphoglycerides see GLYCEROPHOSPHOLIPIDS) or sphingosine (SPHINGOLIPIDS). They are polar lipids that are of great importance for the structure and function of cell membranes and are the most abundant of membrane lipids, although not stored in large amounts in the system.	Phosphatides,Phospholipid
D000418	Albumins	Water-soluble proteins found in egg whites, blood, lymph, and other tissues and fluids. They coagulate upon heating.	Albumin
D013448	Sulfobromophthalein	A phenolphthalein that is used as a diagnostic aid in hepatic function determination.	Bromsulphalein,Bromosulfophthalein,Bromosulphthalein,Bromthalein,Sulfobromophthalein Disodium,Sulfobromophthalein Sodium,Tetrabromsulphthalein,Disodium, Sulfobromophthalein,Sodium, Sulfobromophthalein