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Comparative Analysis of Zika Virus Detection by RT-qPCR, RT-LAMP, and RT-RPA. 2020
Cheikh Tidiane Diagne, and
Martin Faye, and
Benjamin Lopez-Jimena, and
Ahmed Abd El Wahed, and
Cheikh Loucoubar, and
Cheikh Fall, and
Giulia Mencatelli, and
Oumar Faye, and
Ousmane Faye, and
Manfred Weidmann, and
Amadou Alpha Sall
Arbovirus and Viral Haemorrhagic Fever Unit, Institut Pasteur de Dakar, Dakar, Senegal. cheikhtidiane.diagne@pasteur.sn.
Molecular detection of Zika virus (ZIKV) is a key element of outbreak management. Multiple PCR and isothermal ZIKV assays targeting different ZIKV sequences have been published. In this study, we compared a qRT-PCR, 2 RT-LAMP assays (based on different primer design approaches), and an RT-RPA for the detection of African and Asian/American lineages of ZIKV isolates from human, mosquito, and monkey. Results showed that RT-LAMP detected 100% of samples with a time threshold (Tt) of 18.01 ± 11.71 min while qRT-PCR detected 88.88% of samples with a Tt of 58.30 ± 16.58 min and RT-RPA 50% of samples with a Tt of 3.70 ± 0.44 min.
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