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The influence of temperature and ionic strength on the vesiculation properties of large multilamellar vesicles containing various proportions of dimyristoylphosphatidylglycerol has been investigated. It is shown that at low ionic strengths preformed large multilamellar vesicles composed of dimyristoylphosphatidylcholine and dimyristoylphosphatidylglycerol (7:3) on incubation at the gel to liquid-crystalline transition temperature (Tc approximately 23 degrees C) spontaneously vesiculate to form predominantly unilamellar systems with a mean diameter of 120 nm. Such vesiculation is not observed for incubations at temperatures appreciably above or below Tc, and is also inhibited by higher ionic strengths. Stable large multilamellar vesicles are formed, however, in systems containing the dioleoyl species of phosphatidylcholine or phosphatidylglycerol and also for dimyristoylphosphatidylcholine/dimyristoylphosphatidylserine mixtures. The vesiculation properties of dimyristoylphosphatidylcholine/dimyristoylphosphatidylglycerol mixtures, therefore, appear to reflect an instability in the region of the Tc driven by surface potential effects which are specific for the glycerol headgroup.
T D Madden, and
C P Tilcock, and
K Wong, and
P R Cullis
August 1982,
Biochemistry,
T D Madden, and
C P Tilcock, and
K Wong, and
P R Cullis
February 1990,
Chemistry and physics of lipids,
T D Madden, and
C P Tilcock, and
K Wong, and
P R Cullis
April 1980,
Biochemistry,
T D Madden, and
C P Tilcock, and
K Wong, and
P R Cullis
February 2010,
Langmuir : the ACS journal of surfaces and colloids,
T D Madden, and
C P Tilcock, and
K Wong, and
P R Cullis
May 1983,
Biochemistry,
T D Madden, and
C P Tilcock, and
K Wong, and
P R Cullis
October 1981,
Biochemistry,
T D Madden, and
C P Tilcock, and
K Wong, and
P R Cullis
May 1977,
Biochemistry,
T D Madden, and
C P Tilcock, and
K Wong, and
P R Cullis
September 1979,
Biochimica et biophysica acta,
T D Madden, and
C P Tilcock, and
K Wong, and
P R Cullis
October 2003,
Journal of colloid and interface science,
T D Madden, and
C P Tilcock, and
K Wong, and
P R Cullis
October 1982,
Chemistry and physics of lipids,
