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Identification, cloning and heterologous expression of biosynthetic gene cluster for desertomycin. 2020
Takuya Hashimoto, and
Ikuko Kozone, and
Junko Hashimoto, and
Hikaru Suenaga, and
Manabu Fujie, and
Noriyuki Satoh, and
Haruo Ikeda, and
Kazuo Shin-Ya
National Institute of Advanced Industrial Science and Technology, 2-4-7 Aomi, Koto-ku, Tokyo, 135-0064, Japan.
From our in-house microbial genome database of secondary metabolite producers, we identified a candidate biosynthetic gene cluster for desertomycin from Streptomyces nobilis JCM4274. We report herein the cloning of the 127-kb entire gene cluster for desertomycin biosynthesis using bacterial artificial chromosome vector. The entire biosynthetic gene cluster for desertomycin was introduced in the heterologous host, Streptomyces lividans TK23, with an average yield of more than 130 mg l-1.
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