PLK1 regulates hepatic stellate cell activation and liver fibrosis through Wnt/β-catenin signalling pathway. 2020

Yu Chen, and Xin Chen, and Ya-Ru Ji, and Sai Zhu, and Fang-Tian Bu, and Xiao-Sa Du, and Xiao-Ming Meng, and Cheng Huang, and Jun Li
School of Pharmacy, Anhui Key Laboratory of Major Autoimmune Diseases, Anhui Institute of Innovative Drugs, Anhui Medical University, Hefei, China.

As an outcome of chronic liver disease, liver fibrosis involves the activation of hepatic stellate cells (HSCs) caused by a variety of chronic liver injuries. It is important to explore approaches to inhibit the activation and proliferation of HSCs for the treatment of liver fibrosis. PLK1 is overexpressed in many human tumour cells and has become a popular drug target in tumour therapy. Therefore, further study of the function of PLK1 in the cell cycle is valid. In the present study, we found that PLK1 expression was elevated in primary HSCs isolated from CCl4 -induced liver fibrosis mice and LX-2 cells stimulated with TGF-β1. Knockdown of PLK1 inhibited α-SMA and Col1α1 expression and reduced the activation of HSCs in CCl4 -induced liver fibrosis mice and LX-2 cells stimulated with TGF-β1. We further showed that inhibiting the expression of PLK1 reduced the proliferation of HSCs and promoted HSCs apoptosis in vivo and in vitro. Furthermore, we found that the Wnt/β-catenin signalling pathway may be essential for PLK1-mediated HSCs activation. Together, blocking PLK1 effectively suppressed liver fibrosis by inhibiting HSC activation, which may provide a new treatment strategy for liver fibrosis.

UI MeSH Term Description Entries
D008103 Liver Cirrhosis Liver disease in which the normal microcirculation, the gross vascular anatomy, and the hepatic architecture have been variably destroyed and altered with fibrous septa surrounding regenerated or regenerating parenchymal nodules. Cirrhosis, Liver,Fibrosis, Liver,Hepatic Cirrhosis,Liver Fibrosis,Cirrhosis, Hepatic
D008297 Male Males
D008810 Mice, Inbred C57BL One of the first INBRED MOUSE STRAINS to be sequenced. This strain is commonly used as genetic background for transgenic mouse models. Refractory to many tumors, this strain is also preferred model for studying role of genetic variations in development of diseases. Mice, C57BL,Mouse, C57BL,Mouse, Inbred C57BL,C57BL Mice,C57BL Mice, Inbred,C57BL Mouse,C57BL Mouse, Inbred,Inbred C57BL Mice,Inbred C57BL Mouse
D008954 Models, Biological Theoretical representations that simulate the behavior or activity of biological processes or diseases. For disease models in living animals, DISEASE MODELS, ANIMAL is available. Biological models include the use of mathematical equations, computers, and other electronic equipment. Biological Model,Biological Models,Model, Biological,Models, Biologic,Biologic Model,Biologic Models,Model, Biologic
D011518 Proto-Oncogene Proteins Products of proto-oncogenes. Normally they do not have oncogenic or transforming properties, but are involved in the regulation or differentiation of cell growth. They often have protein kinase activity. Cellular Proto-Oncogene Proteins,c-onc Proteins,Proto Oncogene Proteins, Cellular,Proto-Oncogene Products, Cellular,Cellular Proto Oncogene Proteins,Cellular Proto-Oncogene Products,Proto Oncogene Products, Cellular,Proto Oncogene Proteins,Proto-Oncogene Proteins, Cellular,c onc Proteins
D002251 Carbon Tetrachloride A solvent for oils, fats, lacquers, varnishes, rubber waxes, and resins, and a starting material in the manufacturing of organic compounds. Poisoning by inhalation, ingestion or skin absorption is possible and may be fatal. (Merck Index, 11th ed) Tetrachloromethane,Tetrachloride, Carbon
D002460 Cell Line Established cell cultures that have the potential to propagate indefinitely. Cell Lines,Line, Cell,Lines, Cell
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000097869 Polo-Like Kinase 1 Plays a crucial role in the regulation of proliferative activity of normal and malignant cells. Proposed as a new target for antineoplastic treatment strategies. Plk1 protein,STPK13 protein,Polo Like Kinase 1
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia

Related Publications

Yu Chen, and Xin Chen, and Ya-Ru Ji, and Sai Zhu, and Fang-Tian Bu, and Xiao-Sa Du, and Xiao-Ming Meng, and Cheng Huang, and Jun Li
January 2021, European journal of pharmacology,
Yu Chen, and Xin Chen, and Ya-Ru Ji, and Sai Zhu, and Fang-Tian Bu, and Xiao-Sa Du, and Xiao-Ming Meng, and Cheng Huang, and Jun Li
December 2019, European journal of pharmacology,
Yu Chen, and Xin Chen, and Ya-Ru Ji, and Sai Zhu, and Fang-Tian Bu, and Xiao-Sa Du, and Xiao-Ming Meng, and Cheng Huang, and Jun Li
July 2016, Molecular immunology,
Yu Chen, and Xin Chen, and Ya-Ru Ji, and Sai Zhu, and Fang-Tian Bu, and Xiao-Sa Du, and Xiao-Ming Meng, and Cheng Huang, and Jun Li
December 2014, Chemico-biological interactions,
Yu Chen, and Xin Chen, and Ya-Ru Ji, and Sai Zhu, and Fang-Tian Bu, and Xiao-Sa Du, and Xiao-Ming Meng, and Cheng Huang, and Jun Li
October 2015, Chemico-biological interactions,
Yu Chen, and Xin Chen, and Ya-Ru Ji, and Sai Zhu, and Fang-Tian Bu, and Xiao-Sa Du, and Xiao-Ming Meng, and Cheng Huang, and Jun Li
January 2008, American journal of physiology. Gastrointestinal and liver physiology,
Yu Chen, and Xin Chen, and Ya-Ru Ji, and Sai Zhu, and Fang-Tian Bu, and Xiao-Sa Du, and Xiao-Ming Meng, and Cheng Huang, and Jun Li
February 2020, European journal of pharmacology,
Yu Chen, and Xin Chen, and Ya-Ru Ji, and Sai Zhu, and Fang-Tian Bu, and Xiao-Sa Du, and Xiao-Ming Meng, and Cheng Huang, and Jun Li
October 2010, Nature cell biology,
Yu Chen, and Xin Chen, and Ya-Ru Ji, and Sai Zhu, and Fang-Tian Bu, and Xiao-Sa Du, and Xiao-Ming Meng, and Cheng Huang, and Jun Li
February 2022, Liver international : official journal of the International Association for the Study of the Liver,
Yu Chen, and Xin Chen, and Ya-Ru Ji, and Sai Zhu, and Fang-Tian Bu, and Xiao-Sa Du, and Xiao-Ming Meng, and Cheng Huang, and Jun Li
May 2023, Cellular signalling,
Copied contents to your clipboard!