BIOMAT Database Logo BIOMAT Database Logo

Exploiting bacterial outer membrane vesicles as a cross-protective vaccine candidate against avian pathogenic Escherichia coli (APEC). 2020

Rujiu Hu, and Jing Li, and Yuezhen Zhao, and Hua Lin, and Liu Liang, and Mimi Wang, and Haojing Liu, and Yuna Min, and Yupeng Gao, and Mingming Yang
College of Animal Science and Technology, Northwest A&F University, Yangling, 712100, Shaanxi, China.

BACKGROUND The well-known fact that avian pathogenic Escherichia coli (APEC) is harder to prevent due to its numerous serogroups has promoted the development of biological immunostimulatory materials as new vaccine candidates in poultry farms. Bacterial outer membrane vesicles (OMVs), known as spherical nanovesicles enriched with various immunostimulants, are naturally secreted by Gram-negative bacteria, and have gained much attention for developing effective vaccine candidates. Recent report has demonstrated that OMVs of APEC O78 can induce protective immunity in chickens. Here, a novel multi-serogroup OMVs (MOMVs) vaccine was developed to achieve cross-protection against APEC infection in broiler chickens. RESULTS In this study, OMVs produced by three APEC strains were isolated, purified and prepared into MOMVs by mixing these three OMVs. By using SDS-PAGE and LC-MS/MS, 159 proteins were identified in MOMVs and the subcellular location and biological functions of 20 most abundant proteins were analyzed. The immunogenicity of MOMVs was evaluated, and the results showed that MOMVs could elicit innate immune responses, including internalization by chicken macrophage and production of immunomodulatory cytokines. Vaccination with MOMVs induced specific broad-spectrum antibodies as well as Th1 and Th17 immune responses. The animal experiment has confirmed that immunization with an appropriate dose of MOMVs could not cause any adverse effect and was able to reduce bacteria loads and pro-inflammatory cytokines production, thus providing effective cross-protection against lethal infections induced by multi-serogroup APEC strains in chickens. Further experiments indicated that, although vesicular proteins were able to induce stronger protective efficiency than lipopolysaccharide, both vesicular proteins and lipopolysaccharide are crucial in MOMVs-mediated protection. CONCLUSIONS The multi-serogroup nanovesicles produced by APEC strains will open up a new way for the development of next generation vaccines with low toxicity and broad protection in the treatment and control of APEC infection.

UI MeSH Term Description Entries
D008264 Macrophages The relatively long-lived phagocytic cell of mammalian tissues that are derived from blood MONOCYTES. Main types are PERITONEAL MACROPHAGES; ALVEOLAR MACROPHAGES; HISTIOCYTES; KUPFFER CELLS of the liver; and OSTEOCLASTS. They may further differentiate within chronic inflammatory lesions to EPITHELIOID CELLS or may fuse to form FOREIGN BODY GIANT CELLS or LANGHANS GIANT CELLS. (from The Dictionary of Cell Biology, Lackie and Dow, 3rd ed.) Bone Marrow-Derived Macrophages,Monocyte-Derived Macrophages,Macrophage,Macrophages, Monocyte-Derived,Bone Marrow Derived Macrophages,Bone Marrow-Derived Macrophage,Macrophage, Bone Marrow-Derived,Macrophage, Monocyte-Derived,Macrophages, Bone Marrow-Derived,Macrophages, Monocyte Derived,Monocyte Derived Macrophages,Monocyte-Derived Macrophage
D011201 Poultry Diseases Diseases of birds which are raised as a source of meat or eggs for human consumption and are usually found in barnyards, hatcheries, etc. The concept is differentiated from BIRD DISEASES which is for diseases of birds not considered poultry and usually found in zoos, parks, and the wild. Disease, Poultry,Diseases, Poultry,Poultry Disease
D002645 Chickens Common name for the species Gallus gallus, the domestic fowl, in the family Phasianidae, order GALLIFORMES. It is descended from the red jungle fowl of SOUTHEAST ASIA. Gallus gallus,Gallus domesticus,Gallus gallus domesticus,Chicken
D004926 Escherichia coli A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc. Alkalescens-Dispar Group,Bacillus coli,Bacterium coli,Bacterium coli commune,Diffusely Adherent Escherichia coli,E coli,EAggEC,Enteroaggregative Escherichia coli,Enterococcus coli,Diffusely Adherent E. coli,Enteroaggregative E. coli,Enteroinvasive E. coli,Enteroinvasive Escherichia coli
D000080943 Bacterial Outer Membrane The outermost cell envelope of GRAM-NEGATIVE BACTERIA composed of PHOSPHOLIPIDS; LIPOPOLYSACCHARIDES (LPS), and BACTERIAL OUTER MEMBRANE PROTEINS. The bacterial outer membrane LPS demonstrates cytotoxicity (see LIPID A and BACTERIAL O ANTIGEN). Bacterial Outer Membranes,Membrane, Bacterial Outer,Outer Membrane, Bacterial
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D000907 Antibodies, Bacterial Immunoglobulins produced in a response to BACTERIAL ANTIGENS. Bacterial Antibodies
D000942 Antigens, Bacterial Substances elaborated by bacteria that have antigenic activity. Bacterial Antigen,Bacterial Antigens,Antigen, Bacterial
D001425 Bacterial Outer Membrane Proteins Proteins isolated from the outer membrane of Gram-negative bacteria. OMP Proteins,Outer Membrane Proteins, Bacterial,Outer Membrane Lipoproteins, Bacterial
D016207 Cytokines Non-antibody proteins secreted by inflammatory leukocytes and some non-leukocytic cells, that act as intercellular mediators. They differ from classical hormones in that they are produced by a number of tissue or cell types rather than by specialized glands. They generally act locally in a paracrine or autocrine rather than endocrine manner. Cytokine

Related Publications

Rujiu Hu, and Jing Li, and Yuezhen Zhao, and Hua Lin, and Liu Liang, and Mimi Wang, and Haojing Liu, and Yuna Min, and Yupeng Gao, and Mingming Yang
[Avian pathogenic Escherichia coli (APEC)].
January 2003, Berliner und Munchener tierarztliche Wochenschrift,
Rujiu Hu, and Jing Li, and Yuezhen Zhao, and Hua Lin, and Liu Liang, and Mimi Wang, and Haojing Liu, and Yuna Min, and Yupeng Gao, and Mingming Yang
[Avian pathogenic Escherichia coli (APEC)].
October 2002, Tijdschrift voor diergeneeskunde,
Rujiu Hu, and Jing Li, and Yuezhen Zhao, and Hua Lin, and Liu Liang, and Mimi Wang, and Haojing Liu, and Yuna Min, and Yupeng Gao, and Mingming Yang
Avian pathogenic Escherichia coli (APEC).
January 1999, Veterinary research,
Rujiu Hu, and Jing Li, and Yuezhen Zhao, and Hua Lin, and Liu Liang, and Mimi Wang, and Haojing Liu, and Yuna Min, and Yupeng Gao, and Mingming Yang
Outer membrane vesicles as a candidate vaccine against edwardsiellosis.
March 2011, PloS one,
Rujiu Hu, and Jing Li, and Yuezhen Zhao, and Hua Lin, and Liu Liang, and Mimi Wang, and Haojing Liu, and Yuna Min, and Yupeng Gao, and Mingming Yang
Bacterial ghost of avian pathogenic E. coli (APEC) serotype O78:K80 as a homologous vaccine against avian colibacillosis.
January 2018, PloS one,
Rujiu Hu, and Jing Li, and Yuezhen Zhao, and Hua Lin, and Liu Liang, and Mimi Wang, and Haojing Liu, and Yuna Min, and Yupeng Gao, and Mingming Yang
Bacterial outer membrane vesicles as a candidate tumor vaccine platform.
January 2022, Frontiers in immunology,
Rujiu Hu, and Jing Li, and Yuezhen Zhao, and Hua Lin, and Liu Liang, and Mimi Wang, and Haojing Liu, and Yuna Min, and Yupeng Gao, and Mingming Yang
Immunization with outer membrane vesicles of avian pathogenic Escherichia coli O78 induces protective immunity in chickens.
September 2019, Veterinary microbiology,
Rujiu Hu, and Jing Li, and Yuezhen Zhao, and Hua Lin, and Liu Liang, and Mimi Wang, and Haojing Liu, and Yuna Min, and Yupeng Gao, and Mingming Yang
mcr-1 identified in Avian Pathogenic Escherichia coli (APEC).
January 2017, PloS one,
Rujiu Hu, and Jing Li, and Yuezhen Zhao, and Hua Lin, and Liu Liang, and Mimi Wang, and Haojing Liu, and Yuna Min, and Yupeng Gao, and Mingming Yang
Proteome-minimized outer membrane vesicles from Escherichia coli as a generalized vaccine platform.
February 2021, Journal of extracellular vesicles,
Rujiu Hu, and Jing Li, and Yuezhen Zhao, and Hua Lin, and Liu Liang, and Mimi Wang, and Haojing Liu, and Yuna Min, and Yupeng Gao, and Mingming Yang
Avian pathogenic Escherichia coli (APEC) and uropathogenic Escherichia coli (UPEC): characterization and comparison.
July 2021, Journal of infection in developing countries,
Copied contents to your clipboard!