Analysis of TSH receptors and microsomal antigen in different human thyroid tissue specimens. 1988

J Furmaniak, and S Davenport, and Y Nakajima, and U Kaiser, and H W Muller-Gartner, and F W Buckmann, and C Pegg, and B Rees Smith
Endocrine Immunology Unit, University of Wales College of Medicine, Heath Park, Cardiff, UK.

Affinity labelling with a 125I-labelled photoactive derivative of TSH (HSAB-TSH) was used to analyse TSH receptor size in the following specimens of human thyroid tissue: (1) cold nodules; (2) autonomous nodules; (3) papillary carcinoma; (4) medullary carcinoma; (5) metastasis of papillary carcinoma to lymph node; (6) anaplastic carcinoma, and (7) Graves' thyroid. In addition, a sample of histologically normal thyroid tissue surrounding specimens 1-4 was analysed in each case. Thyroid microsomes were also prepared from the tissue samples, solubilized using 1% deoxycholate and labelled with 125I. The preparations were immunoprecipitated using microsomal autoantibodies and protein A and analysed by SDS-PAGE and autoradiography. These studies indicated that no differences in the characteristics of the TSH receptor or of microsomal antigen were observed in the tissue samples 1-3 and 7. Neither protein was detected in tissue specimens 4-6.

UI MeSH Term Description Entries
D008861 Microsomes Artifactual vesicles formed from the endoplasmic reticulum when cells are disrupted. They are isolated by differential centrifugation and are composed of three structural features: rough vesicles, smooth vesicles, and ribosomes. Numerous enzyme activities are associated with the microsomal fraction. (Glick, Glossary of Biochemistry and Molecular Biology, 1990; from Rieger et al., Glossary of Genetics: Classical and Molecular, 5th ed) Microsome
D011989 Receptors, Thyrotropin Cell surface proteins that bind pituitary THYROTROPIN (also named thyroid stimulating hormone or TSH) and trigger intracellular changes of the target cells. TSH receptors are present in the nervous system and on target cells in the thyroid gland. Autoantibodies to TSH receptors are implicated in thyroid diseases such as GRAVES DISEASE and Hashimoto disease (THYROIDITIS, AUTOIMMUNE). Receptors, Thyroid Stimulating Hormone,TSH Receptors,Thyroid Stimulating Hormone Receptors,Thyrotropin Receptors,LATS Receptors,Receptor, LATS Immunoglobulins,Receptors, LATS,Receptors, Long-Acting Thyroid Stimulator,Receptors, TSH,TSH Receptor,Thyroid Stimulating Hormone Receptor,Thyrotropin Receptor,Receptor, TSH,Receptor, Thyrotropin,Receptors, Long Acting Thyroid Stimulator
D004591 Electrophoresis, Polyacrylamide Gel Electrophoresis in which a polyacrylamide gel is used as the diffusion medium. Polyacrylamide Gel Electrophoresis,SDS-PAGE,Sodium Dodecyl Sulfate-PAGE,Gel Electrophoresis, Polyacrylamide,SDS PAGE,Sodium Dodecyl Sulfate PAGE,Sodium Dodecyl Sulfate-PAGEs
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D001324 Autoantigens Endogenous tissue constituents with the ability to interact with AUTOANTIBODIES and cause an immune response. Autoantigen,Autologous Antigen,Autologous Antigens,Self-Antigen,Self-Antigens,Antigen, Autologous,Antigens, Autologous,Self Antigen,Self Antigens
D001345 Autoradiography The making of a radiograph of an object or tissue by recording on a photographic plate the radiation emitted by radioactive material within the object. (Dorland, 27th ed) Radioautography
D013959 Thyroid Diseases Pathological processes involving the THYROID GLAND. Disease, Thyroid,Diseases, Thyroid,Thyroid Disease
D013961 Thyroid Gland A highly vascularized endocrine gland consisting of two lobes joined by a thin band of tissue with one lobe on each side of the TRACHEA. It secretes THYROID HORMONES from the follicular cells and CALCITONIN from the parafollicular cells thereby regulating METABOLISM and CALCIUM level in blood, respectively. Thyroid,Gland, Thyroid,Glands, Thyroid,Thyroid Glands,Thyroids

Related Publications

J Furmaniak, and S Davenport, and Y Nakajima, and U Kaiser, and H W Muller-Gartner, and F W Buckmann, and C Pegg, and B Rees Smith
February 1985, The Journal of surgical research,
J Furmaniak, and S Davenport, and Y Nakajima, and U Kaiser, and H W Muller-Gartner, and F W Buckmann, and C Pegg, and B Rees Smith
April 1990, The Indian journal of medical research,
J Furmaniak, and S Davenport, and Y Nakajima, and U Kaiser, and H W Muller-Gartner, and F W Buckmann, and C Pegg, and B Rees Smith
February 1979, The Journal of clinical endocrinology and metabolism,
J Furmaniak, and S Davenport, and Y Nakajima, and U Kaiser, and H W Muller-Gartner, and F W Buckmann, and C Pegg, and B Rees Smith
December 1989, Endocrinologia experimentalis,
J Furmaniak, and S Davenport, and Y Nakajima, and U Kaiser, and H W Muller-Gartner, and F W Buckmann, and C Pegg, and B Rees Smith
January 1976, Annales de biologie clinique,
J Furmaniak, and S Davenport, and Y Nakajima, and U Kaiser, and H W Muller-Gartner, and F W Buckmann, and C Pegg, and B Rees Smith
January 1981, Horumon to rinsho. Clinical endocrinology,
J Furmaniak, and S Davenport, and Y Nakajima, and U Kaiser, and H W Muller-Gartner, and F W Buckmann, and C Pegg, and B Rees Smith
October 1991, Endocrinologia japonica,
J Furmaniak, and S Davenport, and Y Nakajima, and U Kaiser, and H W Muller-Gartner, and F W Buckmann, and C Pegg, and B Rees Smith
September 1987, Molecular and cellular endocrinology,
J Furmaniak, and S Davenport, and Y Nakajima, and U Kaiser, and H W Muller-Gartner, and F W Buckmann, and C Pegg, and B Rees Smith
November 1985, Hormone and metabolic research = Hormon- und Stoffwechselforschung = Hormones et metabolisme,
J Furmaniak, and S Davenport, and Y Nakajima, and U Kaiser, and H W Muller-Gartner, and F W Buckmann, and C Pegg, and B Rees Smith
January 1990, Acta medica Austriaca,
Copied contents to your clipboard!