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A broad substrate-spectrum beta-lactamase was purified from Flavobacterium meningosepticum GN14059. The purified enzyme gave a single protein band on SDS-polyacrylamide gel electrophoresis. The molecular weight was estimated to be about 30,000 and the isoelectric point was 5.1. The enzyme hydrolyzed various beta-lactam antibiotics including oxyiminocephalosporins and aztreonam. Relative rates, with cephaloridine as 100, were cephalothin 200, cefazolin 48, cefuroxime 153, cefotaxime 51, ceftazidime 20, ampicillin 26, carbenicillin 19, and aztreonam 20. The enzyme activity was inhibited by clavulanic acid, sulbactam, imipenem and cephamycins.
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