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Perforin is one of the cytolytic factors present in the cytoplasmic granules of mouse cytotoxic T lymphocytes and natural killer cells. We have determined the sequence of the N-terminal amino acids of perforin purified from a mouse natural killer cell line, and, by using oligonucleotide probes corresponding to the amino acid residues, we have identified a complementary DNA encoding perforin from the cDNA library of a mouse cytotoxic T lymphocyte clone. As predicted from the functional similarities between perforin and the ninth component of the serum cytolytic system, complement (C9) (refs 4-8), the deduced primary structure of perforin has homology with C9 at their respective functionally conserved regions. We find that perforin is only expressed in killer cell lines, and not in helper T lymphocytes or other tumour cells tested. Thus we have provided direct molecular evidence that a killer-cell-specific protein evolutionally linked to C9 is involved in cell-mediated cytolysis.
Y Shinkai, and
K Takio, and
K Okumura
October 1992,
Zhongguo yi xue ke xue yuan xue bao. Acta Academiae Medicinae Sinicae,
Y Shinkai, and
K Takio, and
K Okumura
December 1983,
Jinrui idengaku zasshi. The Japanese journal of human genetics,
Y Shinkai, and
K Takio, and
K Okumura
January 1989,
Proceedings of the National Academy of Sciences of the United States of America,
Y Shinkai, and
K Takio, and
K Okumura
February 1996,
FEBS letters,
Y Shinkai, and
K Takio, and
K Okumura
July 1986,
The Journal of biological chemistry,
Y Shinkai, and
K Takio, and
K Okumura
November 2006,
Fish & shellfish immunology,
Y Shinkai, and
K Takio, and
K Okumura
June 1974,
The Japanese journal of experimental medicine,
Y Shinkai, and
K Takio, and
K Okumura
January 1974,
International archives of allergy and applied immunology,
Y Shinkai, and
K Takio, and
K Okumura
January 1982,
Proceedings of the National Academy of Sciences of the United States of America,
Y Shinkai, and
K Takio, and
K Okumura
June 1999,
Molecular immunology,
