BIOMAT Database Logo BIOMAT Database Logo

Ambient-temperature primary nonselective enrichment for isolation of Salmonella spp. from an estuarine environment. 1977

J B Kaper, and G S Sayler, and M M Baldini, and R R Colwell

A primary, nonselective, ambient-temperature enrichment procedure for isolation of Salmonella spp. is described. The procedure was superior to elevated-temperature selective enrichment for Salmonella when estuarine water samples were examined. Five Chesapeake Bay stations were monitored, over an 8-month period, for the presence of salmonellae. Of 72 water and sediment samples collected, 17 (23.6%) yielded Salmonella spp. Seven serotypes were identified among the isolates. A seasonal pattern was noted for the incidence of the salmonellae. A most probable number procedure, performed by membrane filtration and nonselective enrichment, yielded Salmonella most probable number indices as high as 110 per 100 g of sediment. The results suggest that new methods, such as the one described in this report, are required for isolation of human intestinal pathogens from estuaries and coastal waters.

UI MeSH Term Description Entries
D007294 Inositol An isomer of glucose that has traditionally been considered to be a B vitamin although it has an uncertain status as a vitamin and a deficiency syndrome has not been identified in man. (From Martindale, The Extra Pharmacopoeia, 30th ed, p1379) Inositol phospholipids are important in signal transduction. Myoinositol,Chiro-Inositol,Mesoinositol,Chiro Inositol
D003470 Culture Media Any liquid or solid preparation made specifically for the growth, storage, or transport of microorganisms or other types of cells. The variety of media that exist allow for the culturing of specific microorganisms and cell types, such as differential media, selective media, test media, and defined media. Solid media consist of liquid media that have been solidified with an agent such as AGAR or GELATIN. Media, Culture
D004376 Galactitol A naturally occurring product of plants obtained following reduction of GALACTOSE. It appears as a white crystalline powder with a slight sweet taste. It may form in excess in the lens of the eye in GALACTOSEMIAS, a deficiency of GALACTOKINASE. Dulcitol
D001431 Bacteriological Techniques Techniques used in studying bacteria. Bacteriologic Technic,Bacteriologic Technics,Bacteriologic Techniques,Bacteriological Technique,Technic, Bacteriological,Technics, Bacteriological,Technique, Bacteriological,Techniques, Bacteriological,Bacteriologic Technique,Bacteriological Technic,Bacteriological Technics,Technic, Bacteriologic,Technics, Bacteriologic,Technique, Bacteriologic,Techniques, Bacteriologic
D012475 Salmonella A genus of gram-negative, facultatively anaerobic, rod-shaped bacteria that utilizes citrate as a sole carbon source. It is pathogenic for humans, causing enteric fevers, gastroenteritis, and bacteremia. Food poisoning is the most common clinical manifestation. Organisms within this genus are separated on the basis of antigenic characteristics, sugar fermentation patterns, and bacteriophage susceptibility.
D012621 Seasons Divisions of the year according to some regularly recurrent phenomena usually astronomical or climatic. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed) Seasonal Variation,Season,Seasonal Variations,Variation, Seasonal,Variations, Seasonal
D012623 Seawater The salinated water of OCEANS AND SEAS that provides habitat for marine organisms. Sea Water,Sea Waters,Seawaters,Water, Sea,Waters, Sea
D012988 Soil Microbiology The presence of bacteria, viruses, and fungi in the soil. This term is not restricted to pathogenic organisms. Microbiology, Soil
D014871 Water Microbiology The presence of bacteria, viruses, and fungi in water. This term is not restricted to pathogenic organisms. Microbiology, Water

Related Publications

J B Kaper, and G S Sayler, and M M Baldini, and R R Colwell
Importance of pre-enrichment media for isolation of Salmonella spp. from swine and poultry.
December 1998, FEMS microbiology letters,
J B Kaper, and G S Sayler, and M M Baldini, and R R Colwell
Comparison of conventional and delayed secondary enrichment for isolation of Salmonella spp. from swine samples.
July 1998, Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc,
J B Kaper, and G S Sayler, and M M Baldini, and R R Colwell
Evaluation of a new enrichment broth for the isolation of Salmonella spp. from poultry products.
September 1997, International journal of food microbiology,
J B Kaper, and G S Sayler, and M M Baldini, and R R Colwell
Enrichment for isolating Salmonella Choleraesuis and other Salmonella spp. from pigs.
August 2003, The Journal of veterinary medical science,
J B Kaper, and G S Sayler, and M M Baldini, and R R Colwell
Protein Chips for Detection of Salmonella spp. from Enrichment Culture.
April 2016, Sensors (Basel, Switzerland),
J B Kaper, and G S Sayler, and M M Baldini, and R R Colwell
A metagenomic analysis of the pre-enrichment step for the isolation of Salmonella spp. from pig feces.
February 2019, Journal of microbiological methods,
J B Kaper, and G S Sayler, and M M Baldini, and R R Colwell
Isolation of Salmonella spp. from Animal Feed.
January 2021, Methods in molecular biology (Clifton, N.J.),
J B Kaper, and G S Sayler, and M M Baldini, and R R Colwell
A Single-Step Enrichment Medium for Nonchromogenic Isolation of Healthy and Cold-Injured Salmonella spp. from Fresh Vegetables.
February 2017, Foodborne pathogens and disease,
J B Kaper, and G S Sayler, and M M Baldini, and R R Colwell
Novobiocin-Salmonella-shigella agar for isolation of Salmonella spp.
January 1996, European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology,
J B Kaper, and G S Sayler, and M M Baldini, and R R Colwell
Multiple plating from enrichment media as an aid to salmonella isolation.
June 1983, The Journal of hygiene,
Copied contents to your clipboard!