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The ontogeny of the pituitary's responsiveness to synthetic rat corticotropin-releasing hormone (CRH) in the late prenatal and early postnatal periods of rats was studied by a superfusion system using whole pituitaries. A significant increase of immunoreactive beta-endorphin (IR-beta-Ep) secretion in response to 10(-10) M CRH but not to 10(-11) M CRH was observed in pituitaries from the 15th day of gestation, the earliest day that we tested, whereas 10(-11) M CRH stimulated IR-beta-Ep release from the pituitaries of 17.5-day-old fetuses. Dose-related IR-beta-Ep secretions induced by 10(-12) M to 10(-10) M CRH were observed in pituitaries of 19.5- and 21.5-day-old fetuses, and 1-, 3- and 9-day-old newborn pups. CRH stimulated not only IR-beta-Ep and IR-adrenocorticotropic hormone (ACTH) but also IR-alpha-melanocyte-stimulating hormone (IR-alpha-MSH) secretions from fetal pituitaries. The content of IR-CRH in the hypothalamic extract from 15-day-old fetus was 6.6 +/- 3.6 pg/hypothalamus (mean +/- S.E.M.) and it gradually increased to reach 212.7 +/- 20.3 pg/hypothalamus on the 21.5th day of gestation. However, the content of IR-CRH in the hypothalamus dramatically decreased just after birth and then rapidly increased again from the 5th day after birth. These data indicate that the responsiveness of corticotrophs to CRH is already present on the 15th day of gestation, when the content of IR-CRH in the hypothalamus is extremely low and that the amount of hypothalamic IR-CRH dramatically dropped for several days just after birth in rats.
M Hotta, and
T Shibasaki, and
A Masuda, and
T Imaki, and
H Demura, and
H Ohno, and
S Daikoku, and
R Benoit, and
N Ling, and
K Shizume
January 1990,
Peritoneal dialysis international : journal of the International Society for Peritoneal Dialysis,
M Hotta, and
T Shibasaki, and
A Masuda, and
T Imaki, and
H Demura, and
H Ohno, and
S Daikoku, and
R Benoit, and
N Ling, and
K Shizume
July 1985,
The Journal of clinical endocrinology and metabolism,
M Hotta, and
T Shibasaki, and
A Masuda, and
T Imaki, and
H Demura, and
H Ohno, and
S Daikoku, and
R Benoit, and
N Ling, and
K Shizume
January 1991,
International journal of developmental neuroscience : the official journal of the International Society for Developmental Neuroscience,
M Hotta, and
T Shibasaki, and
A Masuda, and
T Imaki, and
H Demura, and
H Ohno, and
S Daikoku, and
R Benoit, and
N Ling, and
K Shizume
December 2004,
General and comparative endocrinology,
M Hotta, and
T Shibasaki, and
A Masuda, and
T Imaki, and
H Demura, and
H Ohno, and
S Daikoku, and
R Benoit, and
N Ling, and
K Shizume
June 1991,
Endocrinology and metabolism clinics of North America,
M Hotta, and
T Shibasaki, and
A Masuda, and
T Imaki, and
H Demura, and
H Ohno, and
S Daikoku, and
R Benoit, and
N Ling, and
K Shizume
September 1983,
Nihon Sanka Fujinka Gakkai zasshi,
M Hotta, and
T Shibasaki, and
A Masuda, and
T Imaki, and
H Demura, and
H Ohno, and
S Daikoku, and
R Benoit, and
N Ling, and
K Shizume
May 2001,
Peptides,
M Hotta, and
T Shibasaki, and
A Masuda, and
T Imaki, and
H Demura, and
H Ohno, and
S Daikoku, and
R Benoit, and
N Ling, and
K Shizume
July 1977,
Research in experimental medicine. Zeitschrift fur die gesamte experimentelle Medizin einschliesslich experimenteller Chirurgie,
M Hotta, and
T Shibasaki, and
A Masuda, and
T Imaki, and
H Demura, and
H Ohno, and
S Daikoku, and
R Benoit, and
N Ling, and
K Shizume
March 1984,
The Journal of clinical endocrinology and metabolism,
M Hotta, and
T Shibasaki, and
A Masuda, and
T Imaki, and
H Demura, and
H Ohno, and
S Daikoku, and
R Benoit, and
N Ling, and
K Shizume
March 1983,
Endocrinology,
