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OBJECTIVE Type III collagen plays significant role in skin wound healing, forming provisional matrix guiding the inflammatory cells and fibroblasts into the wound site. Our preliminary study performed on rat's tympanic membrane (TM) using Rat Wound Healing RT2 Profiler PCR Array revealed up-regulated expression of collagen type III α1 chain mRNA also during healing of TM. This study was undertaken to confirm and evaluate collagen type III protein expression and distribution during TM healing process. METHODS Sixty rats were used, of which 10 served as controls and the others had their TM perforated. The experimental animals were divided into five subgroups on the basis of time points (03, 06, 09, 14, 20 day after injury). Videootoscopy and histology were employed to assess morphology of the healing process. The expression of collagen type III was evaluated using Western blot analysis and its tissue localization was determined by the immunohistochemical method. RESULTS The expression of collagen type III remained on the same level as in control TM up to day 06. On day 09 abrupt (p = 0.01) increase of the collagen type III expression was observed and it maintained on the same level to the end of observation period. In perforated TM collagen type III was detected in the healing area along the perforation border and around dilated blood vessels. On day 14 and 20 collagen type III was found in the connective tissue filling up the TM previous defect. CONCLUSIONS Taking into consideration our recent and previous data, as well as results obtained by other authors, is seems possible that the increase of collagen type III expression in the late stage of TM healing contributes to proper scar formation.
M Makuszewska, and
T Bonda, and
M Cieślińska, and
I Bialuk, and
M M Winnicka, and
K Niemczyk
March 2006,
Archives of otolaryngology--head & neck surgery,
M Makuszewska, and
T Bonda, and
M Cieślińska, and
I Bialuk, and
M M Winnicka, and
K Niemczyk
December 2009,
Otology & neurotology : official publication of the American Otological Society, American Neurotology Society [and] European Academy of Otology and Neurotology,
M Makuszewska, and
T Bonda, and
M Cieślińska, and
I Bialuk, and
M M Winnicka, and
K Niemczyk
April 2006,
The Journal of laryngology and otology,
M Makuszewska, and
T Bonda, and
M Cieślińska, and
I Bialuk, and
M M Winnicka, and
K Niemczyk
January 2017,
Tissue engineering. Part A,
M Makuszewska, and
T Bonda, and
M Cieślińska, and
I Bialuk, and
M M Winnicka, and
K Niemczyk
October 1995,
Otolaryngologic clinics of North America,
M Makuszewska, and
T Bonda, and
M Cieślińska, and
I Bialuk, and
M M Winnicka, and
K Niemczyk
April 2024,
Oral diseases,
M Makuszewska, and
T Bonda, and
M Cieślińska, and
I Bialuk, and
M M Winnicka, and
K Niemczyk
January 1971,
Transactions - American Academy of Ophthalmology and Otolaryngology. American Academy of Ophthalmology and Otolaryngology,
M Makuszewska, and
T Bonda, and
M Cieślińska, and
I Bialuk, and
M M Winnicka, and
K Niemczyk
May 1966,
Zeitschrift fur Laryngologie, Rhinologie, Otologie und ihre Grenzgebiete,
M Makuszewska, and
T Bonda, and
M Cieślińska, and
I Bialuk, and
M M Winnicka, and
K Niemczyk
November 1967,
Archives of otolaryngology (Chicago, Ill. : 1960),
M Makuszewska, and
T Bonda, and
M Cieślińska, and
I Bialuk, and
M M Winnicka, and
K Niemczyk
July 2016,
Otolaryngology--head and neck surgery : official journal of American Academy of Otolaryngology-Head and Neck Surgery,
