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The effects of media conditioned by leukemic cells from 11 acute myeloblastic leukemia patients on the growth of autologous blast progenitors were studied. First, it was shown that T-cell-depleted leukemic cells from some patients release high levels of colony-stimulating activity into the culture medium, whereas following further depletion of phagocytic cells, the levels of colony-stimulating activity become undetectable. Second, media conditioned by purified blast cell fraction depleted of both T-cells and phagocytic cells potentiated autologous blast progenitor growth both in methylcellulose and suspension cultures stimulated by optimal concentration of media conditioned by human bladder carcinoma line 5637. Third, media conditioned by these purified blast cells generally did not contain measurable colony-stimulating activity or interleukin 1, whereas substantial levels of granulocyte colony-stimulating factor, granulocyte-macrophage colony-stimulating factor, and interleukin 1 were observed in media conditioned by human bladder carcinoma line 5637 using bioassays and specific immunological assays. Therefore, purified blast cell fraction from acute myeloblastic leukemia patients appears to produce factor(s) other than granulocyte colony-stimulating factor, granulocyte-macrophage colony-stimulating factor, or interleukin 1, which potentiate the growth of autologous blast progenitors both in methylcellulose and suspension cultures.
I Murohashi, and
K Nagata, and
T Suzuki, and
Y Yamashita, and
Y Maruyama, and
N Nara
July 1993,
Experimental hematology,
I Murohashi, and
K Nagata, and
T Suzuki, and
Y Yamashita, and
Y Maruyama, and
N Nara
August 1988,
Blood,
I Murohashi, and
K Nagata, and
T Suzuki, and
Y Yamashita, and
Y Maruyama, and
N Nara
July 1988,
Blood,
I Murohashi, and
K Nagata, and
T Suzuki, and
Y Yamashita, and
Y Maruyama, and
N Nara
January 1981,
Blood cells,
I Murohashi, and
K Nagata, and
T Suzuki, and
Y Yamashita, and
Y Maruyama, and
N Nara
August 1988,
Behring Institute Mitteilungen,
I Murohashi, and
K Nagata, and
T Suzuki, and
Y Yamashita, and
Y Maruyama, and
N Nara
August 1990,
Leukemia,
I Murohashi, and
K Nagata, and
T Suzuki, and
Y Yamashita, and
Y Maruyama, and
N Nara
August 1993,
Blood,
I Murohashi, and
K Nagata, and
T Suzuki, and
Y Yamashita, and
Y Maruyama, and
N Nara
April 1994,
Japanese journal of cancer research : Gann,
I Murohashi, and
K Nagata, and
T Suzuki, and
Y Yamashita, and
Y Maruyama, and
N Nara
September 1991,
Journal of cellular physiology,
I Murohashi, and
K Nagata, and
T Suzuki, and
Y Yamashita, and
Y Maruyama, and
N Nara
January 1986,
Journal of cellular physiology. Supplement,
