Biomaterial Database

Specific direct fluorescent antibody detection of Treponema pallidum. 1977

K C Daniels, and H S Ferneyhough

In a study to determine the reliability of specific direct fluorescent antibody staining of Treponema pallidum (DFATP) in lesion exudate, and to evaluate it as a potential diagnostic test to be used in lieu of or as an adjunct to the darkfield and other laboratory tests as an aid in the diagnosis of early syphilis, two types of comparisons were made: Study A-Replicate specimens from each of 350 lesions were examined in the state laboratory by DFATP and in the field by darkfield microscopy and the results were compared; Study B--95 specimens of lesion exudate were tested by the DFATP test and compared with the Fluorescent Treponemal Antibody Absorption (FTA-ABS) test on patients' sera. The tests in each study were evaluated as to sensitivity, specificity, and agreement with each other. The DFATP proved to be as reliable as the darkfield, was advantageous in some instances, and can be valuable when a darkfield examination is not feasible. Much of the responsibility of an adequate DFATP or darkfield test rests on the one who takes the specimen.

UI	MeSH Term	Description	Entries
D008297	Male		Males
D008722	Methods	A series of steps taken in order to conduct research.	Techniques,Methodological Studies,Methodological Study,Procedures,Studies, Methodological,Study, Methodological,Method,Procedure,Technique
D005122	Exudates and Transudates	Exudates are fluids, CELLS, or other cellular substances that are slowly discharged from BLOOD VESSELS usually from inflamed tissues. Transudates are fluids that pass through a membrane or squeeze through tissue or into the EXTRACELLULAR SPACE of TISSUES. Transudates are thin and watery and contain few cells or PROTEINS.	Transudates,Exudates,Transudates and Exudates,Exudate,Transudate
D005455	Fluorescent Antibody Technique	Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.	Antinuclear Antibody Test, Fluorescent,Coon's Technique,Fluorescent Antinuclear Antibody Test,Fluorescent Protein Tracing,Immunofluorescence Technique,Coon's Technic,Fluorescent Antibody Technic,Immunofluorescence,Immunofluorescence Technic,Antibody Technic, Fluorescent,Antibody Technics, Fluorescent,Antibody Technique, Fluorescent,Antibody Techniques, Fluorescent,Coon Technic,Coon Technique,Coons Technic,Coons Technique,Fluorescent Antibody Technics,Fluorescent Antibody Techniques,Fluorescent Protein Tracings,Immunofluorescence Technics,Immunofluorescence Techniques,Protein Tracing, Fluorescent,Protein Tracings, Fluorescent,Technic, Coon's,Technic, Fluorescent Antibody,Technic, Immunofluorescence,Technics, Fluorescent Antibody,Technics, Immunofluorescence,Technique, Coon's,Technique, Fluorescent Antibody,Technique, Immunofluorescence,Techniques, Fluorescent Antibody,Techniques, Immunofluorescence,Tracing, Fluorescent Protein,Tracings, Fluorescent Protein
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000293	Adolescent	A person 13 to 18 years of age.	Adolescence,Youth,Adolescents,Adolescents, Female,Adolescents, Male,Teenagers,Teens,Adolescent, Female,Adolescent, Male,Female Adolescent,Female Adolescents,Male Adolescent,Male Adolescents,Teen,Teenager,Youths
D000328	Adult	A person having attained full growth or maturity. Adults are of 19 through 44 years of age. For a person between 19 and 24 years of age, YOUNG ADULT is available.	Adults
D013587	Syphilis	A contagious venereal disease caused by the spirochete TREPONEMA PALLIDUM.	Great Pox
D014210	Treponema pallidum	The causative agent of venereal and non-venereal syphilis as well as yaws.

Related Publications

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The detection of Treponema Pallidum by a rapid, direct fluorescent antibody darkfield (DFATP) procedure.

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Detection of Treponema pallidum by a fluorescent monoclonal antibody test.

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Comparison of direct and indirect fluorescent antibody methods for staining Treponema pallidum.

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