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Serum bilirubin has been fractionated by means of a gel-filtration technique, on Bio-Gel P-10 columns and with caffeine solution as eluent. When sera containing high concentration of direct-reacting bilirubin were applied to the column, a bilirubin fraction was eluted with the protein. This bilirubin fraction is not set free from protein in the presence of urea, guanidine and sodium dodecyl sulfate; moreover it exhibits peculiar behaviour in the direct and indirect diazo-reaction and in enzymatic oxidation by bilirubin-oxidase as well. On the basis of these data, such bilirubin fraction has been identified with the previously described delta-bilirubin. A method for the quantitative measurement of delta-bilirubin, based on gel-filtration separation followed by diazo-reaction, has been worked out. Results by this method satisfactorily compared with the Kodak Ektachem method.
C Franzini, and
G Cattozzo, and
A Pagani
January 1980,
Laboratornoe delo,
C Franzini, and
G Cattozzo, and
A Pagani
December 1987,
Clinical biochemistry,
C Franzini, and
G Cattozzo, and
A Pagani
June 1967,
Wiener klinische Wochenschrift,
C Franzini, and
G Cattozzo, and
A Pagani
June 1977,
Archives internationales de pharmacodynamie et de therapie,
C Franzini, and
G Cattozzo, and
A Pagani
January 1976,
Die Pharmazie,
C Franzini, and
G Cattozzo, and
A Pagani
November 1973,
Clinical chemistry,
C Franzini, and
G Cattozzo, and
A Pagani
January 1967,
Laboratornoe delo,
C Franzini, and
G Cattozzo, and
A Pagani
March 1984,
Archiv fur experimentelle Veterinarmedizin,
C Franzini, and
G Cattozzo, and
A Pagani
January 1970,
The Journal of medical laboratory technology,
C Franzini, and
G Cattozzo, and
A Pagani
July 1965,
The Journal of medical laboratory technology,
