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[Determination of specific IgM using column chromatography in immunologic diagnosis of toxoplasmosis]. 1988

F Leone, and I Ilardi
Instituto di malattie infettive, Università La Sapienza, Policlinico Umberto I, Roma.

The usefulness of detecting specific IgM antibodies in the immunodiagnosis of toxoplasmosis is fraught with false positive and false negative results. For instance false positivity can be obtained when rheumatoid factor is present in the serum while false negativity can occur because competing levels of IgG antibodies. We report the results obtained by the use of a simplified chromatographic method, commercially available in kits, for the separation of IgM from IgG in the immunodiagnosis of toxoplasmosis. The 1564 sera tested by indirect immunofluorescent technique (IFA) showed that 119 (7.6%) were positive if the whole serum was tested and only 7 (0.4%) after the chromatographic separation. This suggests that the use of this method can notably reduce the number of false results in sera with high IFA titer of IgM to toxoplasma.

UI MeSH Term Description Entries
D007075 Immunoglobulin M A class of immunoglobulin bearing mu chains (IMMUNOGLOBULIN MU-CHAINS). IgM can fix COMPLEMENT. The name comes from its high molecular weight and originally was called a macroglobulin. Gamma Globulin, 19S,IgM,IgM Antibody,IgM1,IgM2,19S Gamma Globulin,Antibody, IgM
D008297 Male Males
D011237 Predictive Value of Tests In screening and diagnostic tests, the probability that a person with a positive test is a true positive (i.e., has the disease), is referred to as the predictive value of a positive test; whereas, the predictive value of a negative test is the probability that the person with a negative test does not have the disease. Predictive value is related to the sensitivity and specificity of the test. Negative Predictive Value,Positive Predictive Value,Predictive Value Of Test,Predictive Values Of Tests,Negative Predictive Values,Positive Predictive Values,Predictive Value, Negative,Predictive Value, Positive
D011247 Pregnancy The status during which female mammals carry their developing young (EMBRYOS or FETUSES) in utero before birth, beginning from FERTILIZATION to BIRTH. Gestation,Pregnancies
D002845 Chromatography Techniques used to separate mixtures of substances based on differences in the relative affinities of the substances for mobile and stationary phases. A mobile phase (fluid or gas) passes through a column containing a stationary phase of porous solid or liquid coated on a solid support. Usage is both analytical for small amounts and preparative for bulk amounts. Chromatographies
D005260 Female Females
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000913 Antibodies, Protozoan Immunoglobulins produced in a response to PROTOZOAN ANTIGENS. Protozoan Antibodies
D000918 Antibody Specificity The property of antibodies which enables them to react with some ANTIGENIC DETERMINANTS and not with others. Specificity is dependent on chemical composition, physical forces, and molecular structure at the binding site. Antibody Specificities,Specificities, Antibody,Specificity, Antibody
D014123 Toxoplasmosis The acquired form of infection by Toxoplasma gondii in animals and man. Toxoplasma gondii Infection,Infection, Toxoplasma gondii

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