BIOMAT Database Logo BIOMAT Database Logo

Triggering of Apoptosis in Osteosarcoma 143B Cell Line by Carbon Quantum Dots via the Mitochondrial Apoptotic Signal Pathway. 2020

Yang Jiao, and Yimin Guo, and Yingcong Fan, and Rui Wang, and Xiang Li, and Hao Wu, and Zhichao Meng, and Xin Yang, and Yunpeng Cui, and Heng Liu, and Liping Pan, and Talatibaike Maimaitijuma, and Jiazhen Zhang, and Yahong Wang, and Yongping Cao, and Tao Zhang
Department of Orthopedic Surgery, Peking University First Hospital, Beijing 100034, China.

OBJECTIVE Carbon-based nanomaterials have gained attention in the field of biomedicine in recent years, especially for the treatment of complicated diseases such as cancer. Here, we report a novel carbon-based nanomaterial, named carbon quantum dots (CQDs), which has potential for cancer therapy. We performed a systematic study on the effects of CQDs on the osteosarcoma 143B cell line in vitro and in vivo. METHODS Cell counting assay, the neutral red assay, lactic dehydrogenase assay, and fluorescein isothiocyanate (FITC) Annexin V/Propidium iodide (PI) were used to detect the cytotoxicity and apoptosis of CQDs on the 143B cell line. Intracellular reactive oxygen species (ROS) were detected by the oxidation-sensitive fluorescent probe 2',7'-dichlorofluorescein diacetate. The JC-10 assay was used to detect the mitochondrial membrane potential (MMP) of 143B cells incubated with CQDs. The effects of CQDs on the 143B cell line were evaluated by Western blot and immunofluorescence analysis of apoptosis-related proteins Bax, Bcl-2, cytochrome-C, caspase-3, cleaved-caspase-3, PARP1, and cleaved-PARP1. Male tumor-bearing BALB/c nude mice were used to investigate the antitumor effects of CQDs, and the biosafety of CQDs in vivo was tested in male BALB/c mice by measuring weight changes, hematology tests, and histological analyses of major organs. RESULTS CQDs exhibited a high cytotoxicity and induced apoptosis toward the 143B cell line. CQDs can also significantly increase the intracellular level of ROS and lower the mitochondrial membrane potential levels of 143B cells. CQDs increase apoptotic protein expression to induce apoptosis of 143B cells by triggering the mitochondrial apoptotic signaling pathway. The tumor volume in the CQD-treated mice was smaller than that in the control group, the tumor volume inhibition rate was 38.9%, and the inhibitory rate by tumor weight was 30.1%. All biosafety test indexes were within reference ranges, and neither necrosis nor inflammation was observed in major organs. CONCLUSIONS CQDs induced cytotoxicity in the 143B cell line through the mitochondrial apoptotic signaling pathway. CQDs not only showed an antitumor effect but also high biocompatibility in vivo. As a new carbon-based nanomaterial, CQDs usage is a promising method for novel cancer treatments.

UI MeSH Term Description Entries
D008297 Male Males
D008807 Mice, Inbred BALB C An inbred strain of mouse that is widely used in IMMUNOLOGY studies and cancer research. BALB C Mice, Inbred,BALB C Mouse, Inbred,Inbred BALB C Mice,Inbred BALB C Mouse,Mice, BALB C,Mouse, BALB C,Mouse, Inbred BALB C,BALB C Mice,BALB C Mouse
D008819 Mice, Nude Mutant mice homozygous for the recessive gene "nude" which fail to develop a thymus. They are useful in tumor studies and studies on immune responses. Athymic Mice,Mice, Athymic,Nude Mice,Mouse, Athymic,Mouse, Nude,Athymic Mouse,Nude Mouse
D008928 Mitochondria Semiautonomous, self-reproducing organelles that occur in the cytoplasm of all cells of most, but not all, eukaryotes. Each mitochondrion is surrounded by a double limiting membrane. The inner membrane is highly invaginated, and its projections are called cristae. Mitochondria are the sites of the reactions of oxidative phosphorylation, which result in the formation of ATP. They contain distinctive RIBOSOMES, transfer RNAs (RNA, TRANSFER); AMINO ACYL T RNA SYNTHETASES; and elongation and termination factors. Mitochondria depend upon genes within the nucleus of the cells in which they reside for many essential messenger RNAs (RNA, MESSENGER). Mitochondria are believed to have arisen from aerobic bacteria that established a symbiotic relationship with primitive protoeukaryotes. (King & Stansfield, A Dictionary of Genetics, 4th ed) Mitochondrial Contraction,Mitochondrion,Contraction, Mitochondrial,Contractions, Mitochondrial,Mitochondrial Contractions
D002244 Carbon A nonmetallic element with atomic symbol C, atomic number 6, and atomic weight [12.0096; 12.0116]. It may occur as several different allotropes including DIAMOND; CHARCOAL; and GRAPHITE; and as SOOT from incompletely burned fuel. Carbon-12,Vitreous Carbon,Carbon 12,Carbon, Vitreous
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D000970 Antineoplastic Agents Substances that inhibit or prevent the proliferation of NEOPLASMS. Anticancer Agent,Antineoplastic,Antineoplastic Agent,Antineoplastic Drug,Antitumor Agent,Antitumor Drug,Cancer Chemotherapy Agent,Cancer Chemotherapy Drug,Anticancer Agents,Antineoplastic Drugs,Antineoplastics,Antitumor Agents,Antitumor Drugs,Cancer Chemotherapy Agents,Cancer Chemotherapy Drugs,Chemotherapeutic Anticancer Agents,Chemotherapeutic Anticancer Drug,Agent, Anticancer,Agent, Antineoplastic,Agent, Antitumor,Agent, Cancer Chemotherapy,Agents, Anticancer,Agents, Antineoplastic,Agents, Antitumor,Agents, Cancer Chemotherapy,Agents, Chemotherapeutic Anticancer,Chemotherapy Agent, Cancer,Chemotherapy Agents, Cancer,Chemotherapy Drug, Cancer,Chemotherapy Drugs, Cancer,Drug, Antineoplastic,Drug, Antitumor,Drug, Cancer Chemotherapy,Drug, Chemotherapeutic Anticancer,Drugs, Antineoplastic,Drugs, Antitumor,Drugs, Cancer Chemotherapy
D012516 Osteosarcoma A sarcoma originating in bone-forming cells, affecting the ends of long bones. It is the most common and most malignant of sarcomas of the bones, and occurs chiefly among 10- to 25-year-old youths. (From Stedman, 25th ed) Sarcoma, Osteogenic,Osteogenic Sarcoma,Osteosarcoma Tumor,Osteogenic Sarcomas,Osteosarcoma Tumors,Osteosarcomas,Sarcomas, Osteogenic,Tumor, Osteosarcoma,Tumors, Osteosarcoma
D015398 Signal Transduction The intracellular transfer of information (biological activation/inhibition) through a signal pathway. In each signal transduction system, an activation/inhibition signal from a biologically active molecule (hormone, neurotransmitter) is mediated via the coupling of a receptor/enzyme to a second messenger system or to an ion channel. Signal transduction plays an important role in activating cellular functions, cell differentiation, and cell proliferation. Examples of signal transduction systems are the GAMMA-AMINOBUTYRIC ACID-postsynaptic receptor-calcium ion channel system, the receptor-mediated T-cell activation pathway, and the receptor-mediated activation of phospholipases. Those coupled to membrane depolarization or intracellular release of calcium include the receptor-mediated activation of cytotoxic functions in granulocytes and the synaptic potentiation of protein kinase activation. Some signal transduction pathways may be part of larger signal transduction pathways; for example, protein kinase activation is part of the platelet activation signal pathway. Cell Signaling,Receptor-Mediated Signal Transduction,Signal Pathways,Receptor Mediated Signal Transduction,Signal Transduction Pathways,Signal Transduction Systems,Pathway, Signal,Pathway, Signal Transduction,Pathways, Signal,Pathways, Signal Transduction,Receptor-Mediated Signal Transductions,Signal Pathway,Signal Transduction Pathway,Signal Transduction System,Signal Transduction, Receptor-Mediated,Signal Transductions,Signal Transductions, Receptor-Mediated,System, Signal Transduction,Systems, Signal Transduction,Transduction, Signal,Transductions, Signal

Related Publications

Yang Jiao, and Yimin Guo, and Yingcong Fan, and Rui Wang, and Xiang Li, and Hao Wu, and Zhichao Meng, and Xin Yang, and Yunpeng Cui, and Heng Liu, and Liping Pan, and Talatibaike Maimaitijuma, and Jiazhen Zhang, and Yahong Wang, and Yongping Cao, and Tao Zhang
Calycosin induces apoptosis via p38‑MAPK pathway‑mediated activation of the mitochondrial apoptotic pathway in human osteosarcoma 143B cells.
November 2020, Molecular medicine reports,
Yang Jiao, and Yimin Guo, and Yingcong Fan, and Rui Wang, and Xiang Li, and Hao Wu, and Zhichao Meng, and Xin Yang, and Yunpeng Cui, and Heng Liu, and Liping Pan, and Talatibaike Maimaitijuma, and Jiazhen Zhang, and Yahong Wang, and Yongping Cao, and Tao Zhang
Bufalin induces apoptosis in the U‑2OS human osteosarcoma cell line via triggering the mitochondrial pathway.
January 2016, Molecular medicine reports,
Yang Jiao, and Yimin Guo, and Yingcong Fan, and Rui Wang, and Xiang Li, and Hao Wu, and Zhichao Meng, and Xin Yang, and Yunpeng Cui, and Heng Liu, and Liping Pan, and Talatibaike Maimaitijuma, and Jiazhen Zhang, and Yahong Wang, and Yongping Cao, and Tao Zhang
Triggering of apoptosis in osteosarcoma cells by graphene/single-walled carbon nanotube hybrids via the ROS-mediated mitochondrial pathway.
February 2017, Journal of biomedical materials research. Part A,
Yang Jiao, and Yimin Guo, and Yingcong Fan, and Rui Wang, and Xiang Li, and Hao Wu, and Zhichao Meng, and Xin Yang, and Yunpeng Cui, and Heng Liu, and Liping Pan, and Talatibaike Maimaitijuma, and Jiazhen Zhang, and Yahong Wang, and Yongping Cao, and Tao Zhang
Celastrol induces apoptosis of human osteosarcoma cells via the mitochondrial apoptotic pathway.
September 2015, Oncology reports,
Yang Jiao, and Yimin Guo, and Yingcong Fan, and Rui Wang, and Xiang Li, and Hao Wu, and Zhichao Meng, and Xin Yang, and Yunpeng Cui, and Heng Liu, and Liping Pan, and Talatibaike Maimaitijuma, and Jiazhen Zhang, and Yahong Wang, and Yongping Cao, and Tao Zhang
The apoptosis induced by CdTe quantum dots through the mitochondrial pathway in dorsal root ganglion cell line ND7/23.
July 2022, Journal of applied toxicology : JAT,
Yang Jiao, and Yimin Guo, and Yingcong Fan, and Rui Wang, and Xiang Li, and Hao Wu, and Zhichao Meng, and Xin Yang, and Yunpeng Cui, and Heng Liu, and Liping Pan, and Talatibaike Maimaitijuma, and Jiazhen Zhang, and Yahong Wang, and Yongping Cao, and Tao Zhang
ABT-737 potentiates cisplatin-induced apoptosis in human osteosarcoma cells via the mitochondrial apoptotic pathway.
October 2017, Oncology reports,
Yang Jiao, and Yimin Guo, and Yingcong Fan, and Rui Wang, and Xiang Li, and Hao Wu, and Zhichao Meng, and Xin Yang, and Yunpeng Cui, and Heng Liu, and Liping Pan, and Talatibaike Maimaitijuma, and Jiazhen Zhang, and Yahong Wang, and Yongping Cao, and Tao Zhang
CoFe2O4-Quantum Dots for Synergistic Photothermal/Photodynamic Therapy of Non-small-Cell Lung Cancer Via Triggering Apoptosis by Regulating PI3K/AKT Pathway.
July 2021, Nanoscale research letters,
Yang Jiao, and Yimin Guo, and Yingcong Fan, and Rui Wang, and Xiang Li, and Hao Wu, and Zhichao Meng, and Xin Yang, and Yunpeng Cui, and Heng Liu, and Liping Pan, and Talatibaike Maimaitijuma, and Jiazhen Zhang, and Yahong Wang, and Yongping Cao, and Tao Zhang
Mesalazine induces apoptosis via mitochondrial pathway in K562 cell line.
March 2022, Veterinary research forum : an international quarterly journal,
Yang Jiao, and Yimin Guo, and Yingcong Fan, and Rui Wang, and Xiang Li, and Hao Wu, and Zhichao Meng, and Xin Yang, and Yunpeng Cui, and Heng Liu, and Liping Pan, and Talatibaike Maimaitijuma, and Jiazhen Zhang, and Yahong Wang, and Yongping Cao, and Tao Zhang
A polysaccharide from pomegranate peels induces the apoptosis of human osteosarcoma cells via the mitochondrial apoptotic pathway.
August 2014, Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine,
Yang Jiao, and Yimin Guo, and Yingcong Fan, and Rui Wang, and Xiang Li, and Hao Wu, and Zhichao Meng, and Xin Yang, and Yunpeng Cui, and Heng Liu, and Liping Pan, and Talatibaike Maimaitijuma, and Jiazhen Zhang, and Yahong Wang, and Yongping Cao, and Tao Zhang
Correction: CoFe2O4-Quantum Dots for Synergistic Photothermal/Photodynamic Therapy of Non-small-Cell Lung Cancer Via Triggering Apoptosis by Regulating PI3K/AKT Pathway.
August 2023, Discover nano,
Copied contents to your clipboard!