Grape seed proanthocyanidin extract promotes skeletal muscle fiber type transformation via AMPK signaling pathway. 2020

Meng Xu, and Xiaoling Chen, and Zhiqing Huang, and Daiwen Chen, and Bing Yu, and Hong Chen, and Yuheng Luo, and Ping Zheng, and Jie Yu, and Jun He
Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education, Institute of Animal Nutrition, Sichuan Agricultural University, Chengdu, Sichuan 611130, P. R. China.

Transformation of skeletal muscle fiber type from fast twitch to slow twitch has significances for sustained contractile and stretchable events, energy homeostasis and antifatigue ability. However, the regulation of skeletal muscle fiber type transformation through nutritional intervention is still not fully spelled out. Grape seed proanthocyanidin extract (GSPE) has been widely reported to play a broader role in many aspects of diseases with its various pharmacological and health-promoting effects. In this study, we found that GSPE significantly improved the fatigue resistance in mice. GSPE up-regulated slow myosin heavy chain (MyHC) and down-regulated fast MyHC, accompanied by increases in activities of succinic dehydrogenase and malate dehydrogenase and by decreased lactate dehydrogenase activity in muscle of mice and in C2C12 myotubes. The AMP-activated protein kinase (AMPK) signaling can be activated by GSPE. Several upstream and downstream factors of AMPK signaling such as liver kinase B1, nuclear respiratory factor 1, calcium calmodulin-dependent protein kinase kinase β, sirtuin1 and peroxisome proliferator activated receptor-γ coactivator-1α (PGC-1α) were also up-regulated by GSPE. Specific inhibition of AMPK signaling by AMPK inhibitor compound C or by AMPKα1 siRNA significantly abolished the GSPE-induced the activation of AMPK and the increase of PGC-1α, and attenuated the GSPE-induced increase of slow MyHC and decrease of fast MyHC in C2C12 myotubes. Taken together, we revealed that GSPE promotes skeletal muscle fiber type transformation from fast twitch to slow twitch through AMPK signaling pathway, and this GSPE-induced fiber type transformation may contribute to increased fatigue resistance.

UI MeSH Term Description Entries
D008297 Male Males
D008807 Mice, Inbred BALB C An inbred strain of mouse that is widely used in IMMUNOLOGY studies and cancer research. BALB C Mice, Inbred,BALB C Mouse, Inbred,Inbred BALB C Mice,Inbred BALB C Mouse,Mice, BALB C,Mouse, BALB C,Mouse, Inbred BALB C,BALB C Mice,BALB C Mouse
D009119 Muscle Contraction A process leading to shortening and/or development of tension in muscle tissue. Muscle contraction occurs by a sliding filament mechanism whereby actin filaments slide inward among the myosin filaments. Inotropism,Muscular Contraction,Contraction, Muscle,Contraction, Muscular,Contractions, Muscle,Contractions, Muscular,Inotropisms,Muscle Contractions,Muscular Contractions
D002460 Cell Line Established cell cultures that have the potential to propagate indefinitely. Cell Lines,Line, Cell,Lines, Cell
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D015398 Signal Transduction The intracellular transfer of information (biological activation/inhibition) through a signal pathway. In each signal transduction system, an activation/inhibition signal from a biologically active molecule (hormone, neurotransmitter) is mediated via the coupling of a receptor/enzyme to a second messenger system or to an ion channel. Signal transduction plays an important role in activating cellular functions, cell differentiation, and cell proliferation. Examples of signal transduction systems are the GAMMA-AMINOBUTYRIC ACID-postsynaptic receptor-calcium ion channel system, the receptor-mediated T-cell activation pathway, and the receptor-mediated activation of phospholipases. Those coupled to membrane depolarization or intracellular release of calcium include the receptor-mediated activation of cytotoxic functions in granulocytes and the synaptic potentiation of protein kinase activation. Some signal transduction pathways may be part of larger signal transduction pathways; for example, protein kinase activation is part of the platelet activation signal pathway. Cell Signaling,Receptor-Mediated Signal Transduction,Signal Pathways,Receptor Mediated Signal Transduction,Signal Transduction Pathways,Signal Transduction Systems,Pathway, Signal,Pathway, Signal Transduction,Pathways, Signal,Pathways, Signal Transduction,Receptor-Mediated Signal Transductions,Signal Pathway,Signal Transduction Pathway,Signal Transduction System,Signal Transduction, Receptor-Mediated,Signal Transductions,Signal Transductions, Receptor-Mediated,System, Signal Transduction,Systems, Signal Transduction,Transduction, Signal,Transductions, Signal
D044945 Proanthocyanidins Dimers and oligomers of flavan-3-ol units (CATECHIN analogs) linked mainly through C4 to C8 bonds to leucoanthocyanidins. They are structurally similar to ANTHOCYANINS but are the result of a different fork in biosynthetic pathways. Anthocyanidin Polymers,Tannin, Condensed,Condensed Tannin,Condensed Tannins,Procyanidins,Polymers, Anthocyanidin,Tannins, Condensed
D055372 AMP-Activated Protein Kinases Intracellular signaling protein kinases that play a signaling role in the regulation of cellular energy metabolism. Their activity largely depends upon the concentration of cellular AMP which is increased under conditions of low energy or metabolic stress. AMP-activated protein kinases modify enzymes involved in LIPID METABOLISM, which in turn provide substrates needed to convert AMP into ATP. 5'-AMP-Activated Protein Kinase,AMP-Activated Kinase,AMP-Activated Protein Kinase,AMP-Activated Protein Kinase alpha Subunit,AMP-Activated Protein Kinase alpha Subunits,AMP-Activated Protein Kinase beta Subunit,AMP-Activated Protein Kinase beta Subunits,AMP-Activated Protein Kinase gamma Subunit,AMP-Activated Protein Kinase gamma Subunits,PRKAA,5' AMP Activated Protein Kinase,AMP Activated Kinase,AMP Activated Protein Kinase,AMP Activated Protein Kinase alpha Subunit,AMP Activated Protein Kinase alpha Subunits,AMP Activated Protein Kinase beta Subunit,AMP Activated Protein Kinase beta Subunits,AMP Activated Protein Kinase gamma Subunit,AMP Activated Protein Kinase gamma Subunits,AMP Activated Protein Kinases
D056604 Grape Seed Extract Exudate from seeds of the grape plant Vitis vinifera, composed of oils and secondary plant metabolites (BIOFLAVONOIDS and polyphenols) credited with important medicinal properties. Extract, Grape Seed,Seed Extract, Grape
D018485 Muscle Fibers, Skeletal Large, multinucleate single cells, either cylindrical or prismatic in shape, that form the basic unit of SKELETAL MUSCLE. They consist of MYOFIBRILS enclosed within and attached to the SARCOLEMMA. They are derived from the fusion of skeletal myoblasts (MYOBLASTS, SKELETAL) into a syncytium, followed by differentiation. Myocytes, Skeletal,Myotubes,Skeletal Myocytes,Skeletal Muscle Fibers,Fiber, Skeletal Muscle,Fibers, Skeletal Muscle,Muscle Fiber, Skeletal,Myocyte, Skeletal,Myotube,Skeletal Muscle Fiber,Skeletal Myocyte

Related Publications

Meng Xu, and Xiaoling Chen, and Zhiqing Huang, and Daiwen Chen, and Bing Yu, and Hong Chen, and Yuheng Luo, and Ping Zheng, and Jie Yu, and Jun He
April 2024, Journal of food science,
Meng Xu, and Xiaoling Chen, and Zhiqing Huang, and Daiwen Chen, and Bing Yu, and Hong Chen, and Yuheng Luo, and Ping Zheng, and Jie Yu, and Jun He
April 2024, Meat science,
Meng Xu, and Xiaoling Chen, and Zhiqing Huang, and Daiwen Chen, and Bing Yu, and Hong Chen, and Yuheng Luo, and Ping Zheng, and Jie Yu, and Jun He
November 2018, The Journal of nutritional biochemistry,
Meng Xu, and Xiaoling Chen, and Zhiqing Huang, and Daiwen Chen, and Bing Yu, and Hong Chen, and Yuheng Luo, and Ping Zheng, and Jie Yu, and Jun He
August 2021, Nutrition research (New York, N.Y.),
Meng Xu, and Xiaoling Chen, and Zhiqing Huang, and Daiwen Chen, and Bing Yu, and Hong Chen, and Yuheng Luo, and Ping Zheng, and Jie Yu, and Jun He
October 2021, Journal of agricultural and food chemistry,
Meng Xu, and Xiaoling Chen, and Zhiqing Huang, and Daiwen Chen, and Bing Yu, and Hong Chen, and Yuheng Luo, and Ping Zheng, and Jie Yu, and Jun He
January 2024, The Journal of nutritional biochemistry,
Meng Xu, and Xiaoling Chen, and Zhiqing Huang, and Daiwen Chen, and Bing Yu, and Hong Chen, and Yuheng Luo, and Ping Zheng, and Jie Yu, and Jun He
January 2018, Mediators of inflammation,
Meng Xu, and Xiaoling Chen, and Zhiqing Huang, and Daiwen Chen, and Bing Yu, and Hong Chen, and Yuheng Luo, and Ping Zheng, and Jie Yu, and Jun He
January 2015, PloS one,
Meng Xu, and Xiaoling Chen, and Zhiqing Huang, and Daiwen Chen, and Bing Yu, and Hong Chen, and Yuheng Luo, and Ping Zheng, and Jie Yu, and Jun He
April 2022, Animal biotechnology,
Meng Xu, and Xiaoling Chen, and Zhiqing Huang, and Daiwen Chen, and Bing Yu, and Hong Chen, and Yuheng Luo, and Ping Zheng, and Jie Yu, and Jun He
April 2011, Journal of agricultural and food chemistry,
Copied contents to your clipboard!