The absolute positioning accuracy of a robot is an important specification that determines its performance, but it is affected by several error sources. Typical calibration methods only consider kinematic errors and neglect complex non-kinematic errors, thus limiting the absolute positioning accuracy. To further improve the absolute positioning accuracy, we propose an artificial neural network optimized by the differential evolution algorithm. Specifically, the structure and parameters of the network are iteratively updated by differential evolution to improve both accuracy and efficiency. Then, the absolute positioning deviation caused by kinematic and non-kinematic errors is compensated using the trained network. To verify the performance of the proposed network, the simulations and experiments are conducted using a six-degree-of-freedom robot and a laser tracker. The robot average positioning accuracy improved from 0.8497 mm before calibration to 0.0490 mm. The results demonstrate the substantial improvement in the absolute positioning accuracy achieved by the proposed network on an industrial robot.