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An immunohistochemical technique using a commercially available monoclonal antibody to the estrogen receptor in formalin-fixed breast tumors is described. The author's technique is compared to three other recently published techniques in 27 case studies. The authors' method using pronase enzyme pretreatment and alkaline phosphatase as the third antibody yielded the best results. Comparison with the standard dextran-coated charcoal cytosolic assay results from the cases selected yielded a 100% sensitivity and 89% specificity for the authors' technique. The advantages of the immunohistochemical method over the biochemical assay are discussed and clinical implications are suggested. A step-by-step procedure for the authors' technique follows the text.
L Cheng, and
S W Binder, and
Y S Fu, and
K J Lewin
December 1988,
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society,
L Cheng, and
S W Binder, and
Y S Fu, and
K J Lewin
August 2000,
Steroids,
L Cheng, and
S W Binder, and
Y S Fu, and
K J Lewin
November 1990,
Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc,
L Cheng, and
S W Binder, and
Y S Fu, and
K J Lewin
May 1987,
Endocrinology,
L Cheng, and
S W Binder, and
Y S Fu, and
K J Lewin
June 1982,
Cancer,
L Cheng, and
S W Binder, and
Y S Fu, and
K J Lewin
March 2005,
Applied immunohistochemistry & molecular morphology : AIMM,
L Cheng, and
S W Binder, and
Y S Fu, and
K J Lewin
May 1987,
Cancer research,
L Cheng, and
S W Binder, and
Y S Fu, and
K J Lewin
January 1980,
Zentralblatt fur Veterinarmedizin. Reihe A,
L Cheng, and
S W Binder, and
Y S Fu, and
K J Lewin
November 1981,
Ugeskrift for laeger,
L Cheng, and
S W Binder, and
Y S Fu, and
K J Lewin
February 1991,
Hybridoma,
