Biomaterial Database

Isoleucyl-tRNA synthetase inactivation and the extent of aminoacylation of tRNAIle from Escherichia coli. 1977

F Marashi, and C L Harris

A difference in isoleucine acceptance between normal and sulfur-deficient tRNA from Escherichia coli C6 (rel-, met-, cys-) was eliminated when more isoleucyl-tRNA synthetase was added at the reaction plateau. Enzymatic deacylation was similar for both tRNAs. These results suggest that enzyme inactivation caused a premature reaction plateau which was not predicted by the rates of acylation and deacylation.

UI	MeSH Term	Description	Entries
D007533	Isoleucine-tRNA Ligase	An enzyme that activates isoleucine with its specific transfer RNA. EC 6.1.1.5.	Isoleucyl T RNA Synthetase,Isoleucyl- tRNA Synthetase ILS1,Isoleucyl-tRNA Synthetase 1,Isoleucyl-tRNA Synthetase ILES1,Ile-tRNA Ligase,Isoleucyl-tRNA Synthetase,1, Isoleucyl-tRNA Synthetase,ILES1, Isoleucyl-tRNA Synthetase,Ile tRNA Ligase,Isoleucine tRNA Ligase,Isoleucyl tRNA Synthetase,Isoleucyl tRNA Synthetase 1,Isoleucyl tRNA Synthetase ILES1,Isoleucyl tRNA Synthetase ILS1,Ligase, Ile-tRNA,Ligase, Isoleucine-tRNA,Synthetase 1, Isoleucyl-tRNA,Synthetase ILES1, Isoleucyl-tRNA,Synthetase, Isoleucyl-tRNA
D007700	Kinetics	The rate dynamics in chemical or physical systems.
D003545	Cysteine	A thiol-containing non-essential amino acid that is oxidized to form CYSTINE.	Cysteine Hydrochloride,Half-Cystine,L-Cysteine,Zinc Cysteinate,Half Cystine,L Cysteine
D004926	Escherichia coli	A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.	Alkalescens-Dispar Group,Bacillus coli,Bacterium coli,Bacterium coli commune,Diffusely Adherent Escherichia coli,E coli,EAggEC,Enteroaggregative Escherichia coli,Enterococcus coli,Diffusely Adherent E. coli,Enteroaggregative E. coli,Enteroinvasive E. coli,Enteroinvasive Escherichia coli
D000604	Amino Acyl-tRNA Synthetases	A subclass of enzymes that aminoacylate AMINO ACID-SPECIFIC TRANSFER RNA with their corresponding AMINO ACIDS.	Amino Acyl T RNA Synthetases,Amino Acyl-tRNA Ligases,Aminoacyl Transfer RNA Synthetase,Aminoacyl-tRNA Synthetase,Transfer RNA Synthetase,tRNA Synthetase,Acyl-tRNA Ligases, Amino,Acyl-tRNA Synthetases, Amino,Amino Acyl tRNA Ligases,Amino Acyl tRNA Synthetases,Aminoacyl tRNA Synthetase,Ligases, Amino Acyl-tRNA,RNA Synthetase, Transfer,Synthetase, Aminoacyl-tRNA,Synthetase, Transfer RNA,Synthetase, tRNA,Synthetases, Amino Acyl-tRNA
D013455	Sulfur	An element that is a member of the chalcogen family. It has an atomic symbol S, atomic number 16, and atomic weight [32.059; 32.076]. It is found in the amino acids cysteine and methionine.	Sulfur-16,Sulfur 16
D046249	Transfer RNA Aminoacylation	The conversion of uncharged TRANSFER RNA to AMINO ACYL TRNA.	Aminoacylation, Transfer RNA,Transfer RNA Charging,tRNA Aminoacylation,tRNA Charging,Amino Acid Activation, Translational,Transfer RNA Acylation,Transfer RNA Amino Acylation,tRNA Acylation,tRNA Amino Acylation,Acylation, Transfer RNA,Acylation, tRNA,Acylations, Transfer RNA,Acylations, tRNA,Amino Acylation, tRNA,Aminoacylation, tRNA,Aminoacylations, Transfer RNA,Aminoacylations, tRNA,RNA Aminoacylations, Transfer,RNA Charging, Transfer,Transfer RNA Acylations,Transfer RNA Aminoacylations,Transfer RNA Chargings,tRNA Acylations,tRNA Aminoacylations,tRNA Chargings