BIOMAT Database Logo BIOMAT Database Logo

In vitro specular microscope perfusion of M-K- and moist chamber-stored human corneas. 1977

B E McCarey

Human donor corneas were stored in McCarey-Kaufman (M-K) medium for 3 to 6 days; then they were mounted in the specular microscope, and the endothelial surface perfused with a glutathione-bicarbonate-Ringer solution. During storage, the corneal thickness increased 37% above an assumed normal of 0.520 mm. The corneas did not have sufficient active thinning to be accepted as viable when tested by the temperature-reversal phenomenon. Since comparable donor corneas have been used in successful penetrating keratoplasties, there must be a discrepancy between the cell viability as tested by the temperature-reversal phenomenon and clinical application.

UI MeSH Term Description Entries
D008853 Microscopy The use of instrumentation and techniques for visualizing material and details that cannot be seen by the unaided eye. It is usually done by enlarging images, transmitted by light or electron beams, with optical or magnetic lenses that magnify the entire image field. With scanning microscopy, images are generated by collecting output from the specimen in a point-by-point fashion, on a magnified scale, as it is scanned by a narrow beam of light or electrons, a laser, a conductive probe, or a topographical probe. Compound Microscopy,Hand-Held Microscopy,Light Microscopy,Optical Microscopy,Simple Microscopy,Hand Held Microscopy,Microscopy, Compound,Microscopy, Hand-Held,Microscopy, Light,Microscopy, Optical,Microscopy, Simple
D008875 Middle Aged An adult aged 45 - 64 years. Middle Age
D009926 Organ Preservation The process by which organs are kept viable outside of the organism from which they were removed (i.e., kept from decay by means of a chemical agent, cooling, or a fluid substitute that mimics the natural state within the organism). Organ Preservations,Preservation, Organ,Preservations, Organ
D010477 Perfusion Treatment process involving the injection of fluid into an organ or tissue. Perfusions
D011817 Rabbits A burrowing plant-eating mammal with hind limbs that are longer than its fore limbs. It belongs to the family Leporidae of the order Lagomorpha, and in contrast to hares, possesses 22 instead of 24 pairs of chromosomes. Belgian Hare,New Zealand Rabbit,New Zealand Rabbits,New Zealand White Rabbit,Rabbit,Rabbit, Domestic,Chinchilla Rabbits,NZW Rabbits,New Zealand White Rabbits,Oryctolagus cuniculus,Chinchilla Rabbit,Domestic Rabbit,Domestic Rabbits,Hare, Belgian,NZW Rabbit,Rabbit, Chinchilla,Rabbit, NZW,Rabbit, New Zealand,Rabbits, Chinchilla,Rabbits, Domestic,Rabbits, NZW,Rabbits, New Zealand,Zealand Rabbit, New,Zealand Rabbits, New,cuniculus, Oryctolagus
D002415 Cats The domestic cat, Felis catus, of the carnivore family FELIDAE, comprising over 30 different breeds. The domestic cat is descended primarily from the wild cat of Africa and extreme southwestern Asia. Though probably present in towns in Palestine as long ago as 7000 years, actual domestication occurred in Egypt about 4000 years ago. (From Walker's Mammals of the World, 6th ed, p801) Felis catus,Felis domesticus,Domestic Cats,Felis domestica,Felis sylvestris catus,Cat,Cat, Domestic,Cats, Domestic,Domestic Cat
D002470 Cell Survival The span of viability of a cell characterized by the capacity to perform certain functions such as metabolism, growth, reproduction, some form of responsiveness, and adaptability. Cell Viability,Cell Viabilities,Survival, Cell,Viabilities, Cell,Viability, Cell
D003315 Cornea The transparent anterior portion of the fibrous coat of the eye consisting of five layers: stratified squamous CORNEAL EPITHELIUM; BOWMAN MEMBRANE; CORNEAL STROMA; DESCEMET MEMBRANE; and mesenchymal CORNEAL ENDOTHELIUM. It serves as the first refracting medium of the eye. It is structurally continuous with the SCLERA, avascular, receiving its nourishment by permeation through spaces between the lamellae, and is innervated by the ophthalmic division of the TRIGEMINAL NERVE via the ciliary nerves and those of the surrounding conjunctiva which together form plexuses. (Cline et al., Dictionary of Visual Science, 4th ed) Corneas
D004727 Endothelium A layer of epithelium that lines the heart, blood vessels (ENDOTHELIUM, VASCULAR), lymph vessels (ENDOTHELIUM, LYMPHATIC), and the serous cavities of the body. Endotheliums
D005125 Eye Banks Centers for storing various parts of the eye for future use. Bank, Eye,Banks, Eye,Eye Bank

Related Publications

B E McCarey
Dextran flux in M-K medium-stored human corneas.
August 1977, Investigative ophthalmology & visual science,
B E McCarey
Ultrastructural changes in moist chamber corneas.
June 1963, Investigative ophthalmology,
B E McCarey
Corneal transplantation with donor corneas stored in moist chamber and chondroitin sulfate-containing medium.
March 1993, Cornea,
B E McCarey
Specular Microscopy of Human Corneas Stored in an Active Storage Machine.
May 2022, Journal of clinical medicine,
B E McCarey
HISTOCHEMICAL CHANGES IN ENDOTHELIUM OF CORNEAS STORED IN MOIST CHAMBERS.
December 1964, Archives of ophthalmology (Chicago, Ill. : 1960),
B E McCarey
Endothelial changes in rabbit corneas. Observation by specular microscopy during storage in McCarey-Kaufman (M-K) medium.
November 1978, Archives of ophthalmology (Chicago, Ill. : 1960),
B E McCarey
Changes in the corneal Na-K ATPase levels in eyes stored in moist chamber at 4 degrees C.
March 1996, Indian journal of ophthalmology,
B E McCarey
Endothelial cell damage in human and rabbit corneas stored in K-Sol without antioxidants.
October 1989, The British journal of ophthalmology,
B E McCarey
Electron microscope study of the endothelium of stored and cryopreserved monkey corneas.
April 1973, Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie. Albrecht von Graefe's archive for clinical and experimental ophthalmology,
B E McCarey
Epithelialization of the anterior chamber: clinical investigation with the specular microscope.
October 1979, Archives of ophthalmology (Chicago, Ill. : 1960),
Copied contents to your clipboard!