Laboratory evaluation of a rapid four-hour serological grouping of groups A,B,C, and G beta-streptococci by the Phadebact streptococcus test. 1977

R Rosner

Grouping of beta-hemolytic streptococcal isolates by staphylococcal coagglutination was performed with the Phadebact Streptococcus Test to determine whether such isolates could be accurately grouped serologically with 4 h after examination of the primary isolation plates. Of 132 clinical isolates, 131 were correctly grouped by the Phadebact method using the Lancefield precipitation method as the accepted standard. Of the correctly grouped streptococci, 119 were definitively grouped within 4 h after examination of the primary plates, and the remaining 12 isolates were grouped within 24 h. Since the Phadebact Streptococcus Test contains coagglutination reagents for groups A, B, C, and G, those isolates that failed to react were considered as positive for groups other than the four included in the test system. There were 23 such isolates in this study. Lancefield grouping of these isolates indicated that nine were group F, five were group D, and the remaining nine were not groupable with the Lancefield reagents employed in this study. The one Phadebact "failure" involved an isolate that produced a 4 + reaction with the Phadebact group A reagent and a 4 + reaction with Lancefield group F reagent.

UI MeSH Term Description Entries
D000371 Agglutination The clumping together of suspended material resulting from the action of AGGLUTININS.
D012703 Serotyping Process of determining and distinguishing species of bacteria or viruses based on antigens they share. Serotypings
D013291 Streptococcus A genus of gram-positive, coccoid bacteria whose organisms occur in pairs or chains. No endospores are produced. Many species exist as commensals or parasites on man or animals with some being highly pathogenic. A few species are saprophytes and occur in the natural environment.
D013292 Streptococcus agalactiae A bacterium which causes mastitis in cattle and occasionally in man. Streptococcus Group B
D013297 Streptococcus pyogenes A species of gram-positive, coccoid bacteria isolated from skin lesions, blood, inflammatory exudates, and the upper respiratory tract of humans. It is a group A hemolytic Streptococcus that can cause SCARLET FEVER and RHEUMATIC FEVER. Flesh-Eating Bacteria,Streptococcus Group A,Bacteria, Flesh-Eating

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