An epidemiological survey of Theileria equi parasite in donkeys (Equus asinus) in Egypt. 2020

Shimaa Abd El-Salam El-Sayed, and Mahmoud AbouLaila, and Ahmed ElKhatam, and Azaa Abdel-Wahab, and Mohamed Abdo Rizk
Department of Biochemistry and Chemistry of Nutrition, Faculty of Veterinary Medicine, Mansoura University, Mansoura 35516, Egypt.

In the present study, we conducted an epidemiological survey of Theileria equi, with sequencing analysis of the PCR product using blood-DNA samples collected from donkeys (n = 149) reared in different Egyptian provinces in Lower Egypt (Menoufia and Mersa Matruh) and middle Egypt (Giza). All animals were tested for the presence of T. equi parasite using species-specific PCR assay targeting the Equi merozoite antigen-1 (EMA-1). Nine- (6.04%) samples were positive for T. equi. The highest positive rate for infection was detected in Giza zoological garden (10.16%). Egyptian EMA-1 gene sequence exhibited a high identity with gene sequence from Italy, Japan, South Africa, Indian and Israel, the Palestinian Authority. In conclusion, data presented here revealed for the presence of T. equi in donkeys in two provinces of Egypt either in form of acute infection or carriers. These findings have economic significance and indicate the importance of introducing effective prevention and control strategies throughout Egypt to minimize the prevalence of equine piroplasmosis caused by T. equi.

UI MeSH Term Description Entries
D004534 Egypt A country in northern Africa, bordering the Mediterranean Sea, between Libya and the Gaza Strip, and the Red Sea north of Sudan, and includes the Asian Sinai Peninsula Its capital is Cairo. Arab Republic of Egypt,United Arab Republic
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D013801 Theileriasis Infection of cattle, sheep, or goats with protozoa of the genus THEILERIA. This infection results in an acute or chronic febrile condition. Corridor Disease,East Coast Fever,Theileriosis,Coast Fever, East,Disease, Corridor,Fever, East Coast,Theileriases,Theilerioses
D016133 Polymerase Chain Reaction In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships. Anchored PCR,Inverse PCR,Nested PCR,PCR,Anchored Polymerase Chain Reaction,Inverse Polymerase Chain Reaction,Nested Polymerase Chain Reaction,PCR, Anchored,PCR, Inverse,PCR, Nested,Polymerase Chain Reactions,Reaction, Polymerase Chain,Reactions, Polymerase Chain
D016794 Theileria A genus of tick-borne protozoa parasitic in the lymphocytes, erythrocytes, and endothelial cells of mammals. Its organisms multiply asexually and then invade erythrocytes, where they undergo no further reproduction until ingested by a transmitting tick. Theilerias
D018397 Equidae A family of hoofed MAMMALS consisting of HORSES, donkeys, and zebras. Members of this family are strict herbivores and can be classified as either browsers or grazers depending on how they feed. . Asses,Donkeys,Mules,Zebras,Ass,Equus asinus,Donkey,Mule,Zebra

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