BIOMAT Database Logo BIOMAT Database Logo

Destabilization effect of imidazolium cation-Hofmeister anion salts on cytochrome c. 2020

Katarína Garajová, and Dagmar Sedláková, and Martin Berta, and Zuzana Gazova, and Erik Sedlák
Department of Biochemistry, P.J. Šafárik University, Moyzesova 11, 040 01 Košice, Slovakia.

We have analyzed an effect of imidazolium cation-Hofmeister anion salts on stability of basic horse heart cytochrome c (cyt c) at pH4.5 (net charge +17). The effect of salts consisting of imidazolium cations, 1-ethyl-3-methylimidazolium (EMIm+) and 1-butyl-3-methylimidazolium (BMIm+), and five anions: chloride, bromide, iodide, nitrate, and thiocyanate on thermal and pH stability of cyt c was compared with the effect of corresponding sodium salts. Correlation between parameter of dTtrs/d [ion] (Ttrs; thermal midpoints) with surface tension changes of solvent in the presence of both imidazolium and sodium salts implies direct interaction between ions and proteins. Surprisingly, the imidazolium salts have more pronounced destabilization effect on highly positively charged cyt c than the corresponding sodium counterparts. Our analysis suggests the direct interaction of imidazolium cations with polypeptide chain, in analogy to guanidium cation, but the destabilization effect is significantly strengthened by decreased surface tension of imidazolium salt solvents. Comparison of an effect of imidazolium and sodium salts on acidic and alkaline transitions and to thermal transition of cyt c implies a role of hydrophobic interaction between imidazolium cation and polypeptide chain.

UI MeSH Term Description Entries
D007093 Imidazoles Compounds containing 1,3-diazole, a five membered aromatic ring containing two nitrogen atoms separated by one of the carbons. Chemically reduced ones include IMIDAZOLINES and IMIDAZOLIDINES. Distinguish from 1,2-diazole (PYRAZOLES).
D011489 Protein Denaturation Disruption of the non-covalent bonds and/or disulfide bonds responsible for maintaining the three-dimensional shape and activity of the native protein. Denaturation, Protein,Denaturations, Protein,Protein Denaturations
D002412 Cations Positively charged atoms, radicals or groups of atoms which travel to the cathode or negative pole during electrolysis. Cation
D006863 Hydrogen-Ion Concentration The normality of a solution with respect to HYDROGEN ions; H+. It is related to acidity measurements in most cases by pH pH,Concentration, Hydrogen-Ion,Concentrations, Hydrogen-Ion,Hydrogen Ion Concentration,Hydrogen-Ion Concentrations
D000838 Anions Negatively charged atoms, radicals or groups of atoms which travel to the anode or positive pole during electrolysis. Anion
D012492 Salts Substances produced from the reaction between acids and bases; compounds consisting of a metal (positive) and nonmetal (negative) radical. (Grant & Hackh's Chemical Dictionary, 5th ed) Salt
D013500 Surface Tension The force acting on the surface of a liquid, tending to minimize the area of the surface. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed) Interfacial Force,Interfacial Tension,Surface Tensions,Tension, Surface,Tensions, Surface
D045304 Cytochromes c Cytochromes of the c type that are found in eukaryotic MITOCHONDRIA. They serve as redox intermediates that accept electrons from MITOCHONDRIAL ELECTRON TRANSPORT COMPLEX III and transfer them to MITOCHONDRIAL ELECTRON TRANSPORT COMPLEX IV. Cytochrome c,Ferricytochrome c,Ferrocytochrome c,Apocytochrome C
D052578 Ionic Liquids Salts that melt below 100 C. Their low VOLATILIZATION can be an advantage over volatile organic solvents. Ionic Liquid,Ionic Solvents,Liquid, Ionic,Liquids, Ionic,Solvents, Ionic
D055550 Protein Stability The ability of a protein to retain its structural conformation or its activity when subjected to physical or chemical manipulations. Protein Stabilities,Stabilities, Protein,Stability, Protein

Related Publications

Katarína Garajová, and Dagmar Sedláková, and Martin Berta, and Zuzana Gazova, and Erik Sedlák
Cluster structure of imidazolium salts in methanol controlled by the balance of interactions: cation-anion, cation-solvent, and anion-solvent.
October 2008, Analytical sciences : the international journal of the Japan Society for Analytical Chemistry,
Katarína Garajová, and Dagmar Sedláková, and Martin Berta, and Zuzana Gazova, and Erik Sedlák
An internal fluorescent probe based on anthracene to evaluate cation-anion interactions in imidazolium salts.
June 2010, Chemistry (Weinheim an der Bergstrasse, Germany),
Katarína Garajová, and Dagmar Sedláková, and Martin Berta, and Zuzana Gazova, and Erik Sedlák
Influence of substituents on cation-anion contacts in imidazolium perrhenates.
May 2015, Dalton transactions (Cambridge, England : 2003),
Katarína Garajová, and Dagmar Sedláková, and Martin Berta, and Zuzana Gazova, and Erik Sedlák
Surface tensions of imidazolium based ionic liquids: anion, cation, temperature and water effect.
October 2007, Journal of colloid and interface science,
Katarína Garajová, and Dagmar Sedláková, and Martin Berta, and Zuzana Gazova, and Erik Sedlák
Anion complexation and the Hofmeister effect.
October 2014, Angewandte Chemie (International ed. in English),
Katarína Garajová, and Dagmar Sedláková, and Martin Berta, and Zuzana Gazova, and Erik Sedlák
Destabilization of Titania Nanosheet Suspensions by Inorganic Salts: Hofmeister Series and Schulze-Hardy Rule.
July 2017, The journal of physical chemistry. B,
Katarína Garajová, and Dagmar Sedláková, and Martin Berta, and Zuzana Gazova, and Erik Sedlák
Cation and anion effect on the biodegradability and toxicity of imidazolium- and choline-based ionic liquids.
February 2020, Chemosphere,
Katarína Garajová, and Dagmar Sedláková, and Martin Berta, and Zuzana Gazova, and Erik Sedlák
Effects of the Hofmeister anion series salts on the rheological properties of Sesbania cannabina galactomannan.
October 2021, International journal of biological macromolecules,
Katarína Garajová, and Dagmar Sedláková, and Martin Berta, and Zuzana Gazova, and Erik Sedlák
Cation-Anion-CO2 Interactions in Imidazolium-Based Ionic Liquid Sorbents.
November 2018, Chemphyschem : a European journal of chemical physics and physical chemistry,
Katarína Garajová, and Dagmar Sedláková, and Martin Berta, and Zuzana Gazova, and Erik Sedlák
[Effect of salts on the spectral characteristics of immobilizied cytochrome c].
January 1977, Biofizika,
Copied contents to your clipboard!