Cholesterol plays a vital role in a human body. It is known as one of the most important sterols, because it forms cell walls and participates in signal transduction. Moreover, cholesterol was recognized as biomarker of cardiovascular diseases and of some metabolic disorders. As a result, cholesterol blood levels should be controlled in a variety of diseases such as ischemic heart disease, cerebrovascular ischemia, stroke, hypertension, type II diabetes, and many others. Hence, the accurate cholesterol quantification plays an important role in diagnosis and treatment of these diseases. Modern voltammetric and amperometric methods are increasingly used for cholesterol monitoring. Consequently, the problem of electrode fabrication for cholesterol detection has high importance for clinical tests. Novel electrode materials initiated the fast growth of electrochemical biosensors. Biomaterials are still the most frequently used modifiers for cholesterol sensors due to their high selectivity. However, biomaterials have low stability complicating their practical applications. This fact is crucial for analytical parameters such as limit of detection (LOD) and sensitivity. Therefore, nanomaterials are used to eliminate disadvantages of biomaterials and to improve sensors performance by increasing the electrode surface, conductivity and sensitivity. This review is focused on the use of non-enzymatic electrodes for cholesterol quantification and on different approaches to their fabrication. Firstly, the necessity and role of modifier is discussed. Afterwards, the advantages and disadvantages of currently used modifiers are critically compared together with all aspects and approaches to sensors fabrication. Finally, the prospects of non-enzymatic electrodes application for cholesterol sensors engineering are summarised.