Biomaterial Database

Quantitative determination of Escherichia coli from faecal coliforms in seawater. 1988

A Mates, and M Schaffer

Public Health Laboratory, Ministry of Health, Haifa, Israel.

A simple rapid method was developed for counting Escherichia coli in sea water using a membrane filter procedure. Following filtration the membrane filter was incubated on mFC agar medium for 24 +/- 2 h at 44.5 +/- 0.2 degrees C for determination of faecal coliforms. An in situ test for the determination of E. coli was carried out by transferring the membrane filter to nutrient agar containing 4,methyl-umbiliferyl-B-D, glucoronide, followed by incubation for 3 h at 35 degrees C. The E. coli colonies were detected by fluorescence under long wavelength UV light. Extensive biochemical confirmation tests on the isolates showed that all the fluorescence colonies which were either lactose positive or negative were E. coli.

UI	MeSH Term	Description	Entries
D004755	Enterobacteriaceae	A family of gram-negative, facultatively anaerobic, rod-shaped bacteria that do not form endospores. Its organisms are distributed worldwide with some being saprophytes and others being plant and animal parasites. Many species are of considerable economic importance due to their pathogenic effects on agriculture and livestock.	Coliform Bacilli,Enterobacteria,Ewingella,Leclercia,Paracolobactrum,Sodalis
D004926	Escherichia coli	A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.	Alkalescens-Dispar Group,Bacillus coli,Bacterium coli,Bacterium coli commune,Diffusely Adherent Escherichia coli,E coli,EAggEC,Enteroaggregative Escherichia coli,Enterococcus coli,Diffusely Adherent E. coli,Enteroaggregative E. coli,Enteroinvasive E. coli,Enteroinvasive Escherichia coli
D005243	Feces	Excrement from the INTESTINES, containing unabsorbed solids, waste products, secretions, and BACTERIA of the DIGESTIVE SYSTEM.
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D012623	Seawater	The salinated water of OCEANS AND SEAS that provides habitat for marine organisms.	Sea Water,Sea Waters,Seawaters,Water, Sea,Waters, Sea
D012722	Sewage	Refuse liquid or waste matter carried off by sewers.	Sludge,Sludge Flocs
D014871	Water Microbiology	The presence of bacteria, viruses, and fungi in water. This term is not restricted to pathogenic organisms.	Microbiology, Water