BIOMAT Database Logo BIOMAT Database Logo

Purification and partial characterization of two proteinases from Clostridium butyricum M 55. 1988

P Ciborowski, and W Hryniewicz, and J Jeljaszewicz
Department of Bacteriology, National Institute of Hygiene, Warsaw, Poland.

Clostridium butyricum M55 proteinases were purified by application of a multistep procedure involving ethanol precipitation, DEAE cellulose chromatography and molecular sieving. The purified enzymes obtained were called proteinase I and proteinase II. They appeared to be homogeneous when examined by molecular sieving and polyacrylamide gel electrophoresis. The highly purified proteinases were studied for their physico-chemical properties. The influences of pH, temperature, ionic strength and amino acids composition were investigated. The effects of metal ions and of protein-structure-modifying agents support views suggesting the character of these enzymes.

UI MeSH Term Description Entries
D008970 Molecular Weight The sum of the weight of all the atoms in a molecule. Molecular Weights,Weight, Molecular,Weights, Molecular
D010450 Endopeptidases A subclass of PEPTIDE HYDROLASES that catalyze the internal cleavage of PEPTIDES or PROTEINS. Endopeptidase,Peptide Peptidohydrolases
D002848 Chromatography, DEAE-Cellulose A type of ion exchange chromatography using diethylaminoethyl cellulose (DEAE-CELLULOSE) as a positively charged resin. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed) DEAE-Cellulose Chromatography,Chromatography, DEAE Cellulose,DEAE Cellulose Chromatography
D002850 Chromatography, Gel Chromatography on non-ionic gels without regard to the mechanism of solute discrimination. Chromatography, Exclusion,Chromatography, Gel Permeation,Chromatography, Molecular Sieve,Gel Filtration,Gel Filtration Chromatography,Chromatography, Size Exclusion,Exclusion Chromatography,Gel Chromatography,Gel Permeation Chromatography,Molecular Sieve Chromatography,Chromatography, Gel Filtration,Exclusion Chromatography, Size,Filtration Chromatography, Gel,Filtration, Gel,Sieve Chromatography, Molecular,Size Exclusion Chromatography
D003013 Clostridium A genus of motile or nonmotile gram-positive bacteria of the family Clostridiaceae. Many species have been identified with some being pathogenic. They occur in water, soil, and in the intestinal tract of humans and lower animals.
D004591 Electrophoresis, Polyacrylamide Gel Electrophoresis in which a polyacrylamide gel is used as the diffusion medium. Polyacrylamide Gel Electrophoresis,SDS-PAGE,Sodium Dodecyl Sulfate-PAGE,Gel Electrophoresis, Polyacrylamide,SDS PAGE,Sodium Dodecyl Sulfate PAGE,Sodium Dodecyl Sulfate-PAGEs
D006863 Hydrogen-Ion Concentration The normality of a solution with respect to HYDROGEN ions; H+. It is related to acidity measurements in most cases by pH pH,Concentration, Hydrogen-Ion,Concentrations, Hydrogen-Ion,Hydrogen Ion Concentration,Hydrogen-Ion Concentrations
D000596 Amino Acids Organic compounds that generally contain an amino (-NH2) and a carboxyl (-COOH) group. Twenty alpha-amino acids are the subunits which are polymerized to form proteins. Amino Acid,Acid, Amino,Acids, Amino
D013696 Temperature The property of objects that determines the direction of heat flow when they are placed in direct thermal contact. The temperature is the energy of microscopic motions (vibrational and translational) of the particles of atoms. Temperatures

Related Publications

P Ciborowski, and W Hryniewicz, and J Jeljaszewicz
Partial purification and properties of acyl-CoA reductase from Clostridium butyricum.
September 1978, Archives of biochemistry and biophysics,
P Ciborowski, and W Hryniewicz, and J Jeljaszewicz
[Sporulation and germination in Clostridium butyricum M 55. Electron microscopic studies].
November 1966, Zentralblatt fur Bakteriologie, Parasitenkunde, Infektionskrankheiten und Hygiene. 1. Abt. Medizinisch-hygienische Bakteriologie, Virusforschung und Parasitologie. Originale,
P Ciborowski, and W Hryniewicz, and J Jeljaszewicz
Purification and characterization of a new beta-lactamase from Clostridium butyricum.
August 1989, Antimicrobial agents and chemotherapy,
P Ciborowski, and W Hryniewicz, and J Jeljaszewicz
[Cultivation of Clostridium butyricum, Strain M 55. II. Cultivation in meat extract media].
January 1971, Zentralblatt fur Bakteriologie, Parasitenkunde, Infektionskrankheiten und Hygiene. 1. Abt. Medizinisch-hygienische Bakteriologie, Virusforschung und Parasitologie. Originale,
P Ciborowski, and W Hryniewicz, and J Jeljaszewicz
[Cultivation of Clostridium butyricum M 55 strain. 1. Cultivation in peptone-containing media].
January 1970, Zentralblatt fur Bakteriologie, Parasitenkunde, Infektionskrankheiten und Hygiene. 1. Abt. Medizinisch-hygienische Bakteriologie, Virusforschung und Parasitologie. Originale,
P Ciborowski, and W Hryniewicz, and J Jeljaszewicz
[Tumor hyperthermia using high frequency for increase of oncolysis by clostridium butyricum (M 55)].
December 1976, Strahlentherapie,
P Ciborowski, and W Hryniewicz, and J Jeljaszewicz
Partial purification and characterization of a cytotoxin from Clostridium difficile.
January 1979, Reviews of infectious diseases,
P Ciborowski, and W Hryniewicz, and J Jeljaszewicz
Purification and partial characterization of collagenolytic enzymes from Clostridium histolyticum.
March 1968, Biochemistry,
P Ciborowski, and W Hryniewicz, and J Jeljaszewicz
Purification and partial characterization of proline dehydrogenase from Clostridium sporogenes.
September 1981, Canadian journal of microbiology,
P Ciborowski, and W Hryniewicz, and J Jeljaszewicz
Purification and characterization of konjac glucomannan degrading enzyme from anaerobic human intestinal bacterium, Clostridium butyricum-Clostridium beijerinckii group.
October 1997, Bioscience, biotechnology, and biochemistry,
Copied contents to your clipboard!