[Assessment of bacterial counts in the meat-processing industry. II. The plate-loop method and the droplet method (author's transl)]. 1977

G E Gerats, and J M Snijders

Two methods used in making bacterial counts of meat and surfaces are discussed. When the plate-loop method is used, dilutions are prepared with calibrated loops in spotting tiles. These loops are also used to swab droplets of these dilutions on quadrants of previously dried agar plates. A formula is presented, which may be used in calculating bacterial counts. When the droplet method is adopted, an apparatus is used to prepare dilutions in tubes containing liquid agar. Drops of agar then are transferred into empty Petri dishes by this apparatus. After incubation, the colonies which have grown in these droplets are counted by a viewer included in the apparatus. Both the plate-loop method and the droplet method (if slightly modified) were found to satisfy the criteria simplicity, reliability and saving time and material. This only holds good when accurate counts are made and when bacterial counts are more than 2,500 bacteria per gram or per sq. cm. When these conditions are complied with, the two methods are suited for routine studies of large numbers of samples.

UI MeSH Term Description Entries
D008460 Meat The edible portions of any animal used for food including cattle, swine, goats/sheep, poultry, fish, shellfish, and game. Meats
D008462 Meat-Packing Industry The aggregate enterprise of technically producing packaged meat. Industry, Meat-Packing,Industries, Meat-Packing,Industry, Meat Packing,Meat Packing Industry,Meat-Packing Industries
D002417 Cattle Domesticated bovine animals of the genus Bos, usually kept on a farm or ranch and used for the production of meat or dairy products or for heavy labor. Beef Cow,Bos grunniens,Bos indicus,Bos indicus Cattle,Bos taurus,Cow,Cow, Domestic,Dairy Cow,Holstein Cow,Indicine Cattle,Taurine Cattle,Taurus Cattle,Yak,Zebu,Beef Cows,Bos indicus Cattles,Cattle, Bos indicus,Cattle, Indicine,Cattle, Taurine,Cattle, Taurus,Cattles, Bos indicus,Cattles, Indicine,Cattles, Taurine,Cattles, Taurus,Cow, Beef,Cow, Dairy,Cow, Holstein,Cows,Dairy Cows,Domestic Cow,Domestic Cows,Indicine Cattles,Taurine Cattles,Taurus Cattles,Yaks,Zebus
D005516 Food Microbiology The presence of bacteria, viruses, and fungi in food and food products. This term is not restricted to pathogenic organisms: the presence of various non-pathogenic bacteria and fungi in cheeses and wines, for example, is included in this concept. Microbiology, Food
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D001431 Bacteriological Techniques Techniques used in studying bacteria. Bacteriologic Technic,Bacteriologic Technics,Bacteriologic Techniques,Bacteriological Technique,Technic, Bacteriological,Technics, Bacteriological,Technique, Bacteriological,Techniques, Bacteriological,Bacteriologic Technique,Bacteriological Technic,Bacteriological Technics,Technic, Bacteriologic,Technics, Bacteriologic,Technique, Bacteriologic,Techniques, Bacteriologic

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