Purification of metacyclic trypomastigotes of Trypanosoma cruzi and Trypanosoma dionisii from culture using an epimastigote-specific monoclonal antibody. 1987

K Petry, and J Schottelius, and T Baltz

Monoclonal antibodies which react with culture forms of Trypanosoma dionisii and Trypanosoma cruzi were tested for their agglutination capacity. In these studies developmental stage specificity for the epimastigote form could be observed. This specificity meant it was possible to develop a quick and simple method to isolate cultured metacyclic trypomastigotes of T. dionisii and T. cruzi group 2. After agglutination of the epimastigote form with the monoclonal antibody Dion 4.6 the purity of metacyclic trypomastigote developmental forms derived from culture was 96% to 99%.

UI MeSH Term Description Entries
D000371 Agglutination The clumping together of suspended material resulting from the action of AGGLUTININS.
D000372 Agglutination Tests Tests that are dependent on the clumping of cells, microorganisms, or particles when mixed with specific antiserum. (From Stedman, 26th ed) Agglutination Test,Test, Agglutination,Tests, Agglutination
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D000911 Antibodies, Monoclonal Antibodies produced by a single clone of cells. Monoclonal Antibodies,Monoclonal Antibody,Antibody, Monoclonal
D014345 Trypanosoma A genus of flagellate protozoans found in the BLOOD and LYMPH of vertebrates and invertebrates, both hosts being required to complete the life cycle. Nannomonas,Trypanosomes,Nannomona,Trypanosome
D014349 Trypanosoma cruzi The agent of South American trypanosomiasis or CHAGAS DISEASE. Its vertebrate hosts are man and various domestic and wild animals. Insects of several species are vectors. Trypanosoma cruzus,cruzi, Trypanosoma

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