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Ultrathin cryosections of segments of growth plates from rat rib and isolated, gelatin-embedded chondrocytes from the same source were used to demonstrate the ultrastructural location of IGF-I in the different layers of the growth plate. Sections were incubated with a polyclonal rabbit antiserum to free IGF-I and colloidal gold coated with goat anti-rabbit IgGs. The gold label was present in all chondrocytes, localized almost exclusively to the cytoplasm. Particularly the cells in the proliferating zone displayed considerable amounts of gold label. In these cells, gold label was predominantly present over the rough endoplasmic reticulum and membrane bound vesicles, with only negligible gold label over the nuclei and cartilage matrix. The distribution of gold label in the chondrocytes from the proliferating zone, especially in the rough endoplasmic reticulum, strongly suggests that IGF-I can be synthesized by these cells. However, further studies are needed to verify whether there is a local synthesis of IGF-I in the chondrocytes and whether it is under control of growth hormone.
J Wroblewski, and
M Engström, and
A Skottner, and
K Madsen, and
U Friberg
June 1974,
[Osaka Daigaku shigaku zasshi] The journal of Osaka University Dental Society,
J Wroblewski, and
M Engström, and
A Skottner, and
K Madsen, and
U Friberg
December 1988,
Experimental cell research,
J Wroblewski, and
M Engström, and
A Skottner, and
K Madsen, and
U Friberg
August 1989,
Cell biology international reports,
J Wroblewski, and
M Engström, and
A Skottner, and
K Madsen, and
U Friberg
August 1986,
Science (New York, N.Y.),
J Wroblewski, and
M Engström, and
A Skottner, and
K Madsen, and
U Friberg
January 2000,
Connective tissue research,
J Wroblewski, and
M Engström, and
A Skottner, and
K Madsen, and
U Friberg
February 1992,
Cell biology international reports,
J Wroblewski, and
M Engström, and
A Skottner, and
K Madsen, and
U Friberg
March 1995,
Nihon geka hokan. Archiv fur japanische Chirurgie,
J Wroblewski, and
M Engström, and
A Skottner, and
K Madsen, and
U Friberg
January 1995,
Connective tissue research,
J Wroblewski, and
M Engström, and
A Skottner, and
K Madsen, and
U Friberg
December 1975,
Calcified tissue research,
J Wroblewski, and
M Engström, and
A Skottner, and
K Madsen, and
U Friberg
October 2010,
Molecular and cellular endocrinology,
