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A radioimmunoassay (RIA) for human pro-LRF(14-69)OH was developed with an antiserum, generated in a rabbit, to [Tyr67]pro-LRF(47-67)NH2 conjugated to BSA. This antiserum bound 28-32% of [125I]pro-LRF(14-69)OH at a final dilution of 1:2500 and the binding was inhibited by pro-LRF(14-69)OH in a dose-dependent manner. The sensitivity of the RIA was 31.2-62.5 pg and the dose that inhibited 50% of the binding to the tracer was 280-320 pg. Intra- and inter-assay coefficients of variation at 50% inhibition were 8 and 12%, respectively. Neither LRF nor pro-LRF(14-37)OH was recognized by the antiserum. The dilution curve generated with human hypothalamic extract was parallel to that of pro-LRF(14-69)OH. In addition the extract yielded a major immunoreactive peak emerging in elution volumes concordant with [125I]pro-LRF(14-69)OH on Sephadex G-50 chromatography.
A Motoyama, and
I Wakabayashi, and
S Minami, and
H Sugihara, and
F Takahashi, and
S Akira, and
N Ling
September 1974,
The Journal of clinical endocrinology and metabolism,
A Motoyama, and
I Wakabayashi, and
S Minami, and
H Sugihara, and
F Takahashi, and
S Akira, and
N Ling
April 1973,
The Journal of endocrinology,
A Motoyama, and
I Wakabayashi, and
S Minami, and
H Sugihara, and
F Takahashi, and
S Akira, and
N Ling
September 1979,
Fertility and sterility,
A Motoyama, and
I Wakabayashi, and
S Minami, and
H Sugihara, and
F Takahashi, and
S Akira, and
N Ling
January 1977,
Advances in experimental medicine and biology,
A Motoyama, and
I Wakabayashi, and
S Minami, and
H Sugihara, and
F Takahashi, and
S Akira, and
N Ling
October 1972,
Nihon Naibunpi Gakkai zasshi,
A Motoyama, and
I Wakabayashi, and
S Minami, and
H Sugihara, and
F Takahashi, and
S Akira, and
N Ling
August 1973,
The Journal of clinical endocrinology and metabolism,
A Motoyama, and
I Wakabayashi, and
S Minami, and
H Sugihara, and
F Takahashi, and
S Akira, and
N Ling
January 1975,
Neuroendocrinology,
A Motoyama, and
I Wakabayashi, and
S Minami, and
H Sugihara, and
F Takahashi, and
S Akira, and
N Ling
February 1973,
Biochemical and biophysical research communications,
A Motoyama, and
I Wakabayashi, and
S Minami, and
H Sugihara, and
F Takahashi, and
S Akira, and
N Ling
October 1964,
Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N.Y.),
A Motoyama, and
I Wakabayashi, and
S Minami, and
H Sugihara, and
F Takahashi, and
S Akira, and
N Ling
January 1974,
Acta obstetricia et gynecologica Scandinavica,
