In vitro effects of selected endocrine disruptors (DEHP, PCB118, BPA) on narrow-clawed crayfish (Astacus leptodactylus) primary cells. 2020

B Yurdakok-Dikmen, and Y Turgut, and A Ç Gunal, and R Uyar, and O Kuzukıran, and A Filazi, and F Erkoc
Faculty of Veterinary Medicine, Department of Pharmacology and Toxicology, Ankara University, 06110, Diskapi, Ankara, Turkey. byurdakok@yahoo.com.

Environmental pollutants with endocrine-disrupting effect are of global importance due to their contribution to the aethiologies of variety of complex diseases. These lipophilic pollutants are persistent in the environment and able to bioaccummulate in nontarget organisms. BPA, DEHP and PCB118 (dioxin-like PCB) are associated with endocrine disruption effects, while information on their effects on aquatic invertebrates are limited. In the current study, the effects of these compounds, which are ubiqutous and present at low concentrations in the environment, are studied in the primary hepatopancreas, muscle, gill, intestine and gonadal cultures of narrow-clawed crayfish (Astacus leptodactylus Eschscholtz, 1823), a widely distributed freshwater crayfish in Turkey with high economic importance. IC50 values following MTT assay ranged 0.27-12.61 nM; when compared with other tissues, the gonads were more affected with lower IC50 values. PCB118 induced higher cytotoxicity, while DEHP was the least toxic compound. This is the first study on the primary culture of A. leptodactylus¸ and the toxic effects of these compounds in this organism providing mechanistic insights on the responses and detoxification capacity of the organs. This study provides basis to unravel the mechanism of action of the tested EDCs in crayfish and improvement of cell culture conditions for ecotoxicity and screening assays.

UI MeSH Term Description Entries
D010636 Phenols Benzene derivatives that include one or more hydroxyl groups attached to the ring structure.
D011078 Polychlorinated Biphenyls Industrial products consisting of a mixture of chlorinated biphenyl congeners and isomers. These compounds are highly lipophilic and tend to accumulate in fat stores of animals. Many of these compounds are considered toxic and potential environmental pollutants. PCBs,Polychlorinated Biphenyl,Polychlorobiphenyl Compounds,Biphenyl, Polychlorinated,Biphenyls, Polychlorinated,Compounds, Polychlorobiphenyl
D002478 Cells, Cultured Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others. Cultured Cells,Cell, Cultured,Cultured Cell
D003400 Astacoidea A superfamily of various freshwater CRUSTACEA, in the infraorder Astacidea, comprising the crayfish. Common genera include Astacus and Procambarus. Crayfish resemble lobsters, but are usually much smaller. Astacus,Crayfish,Procambarus,Astacoideas,Crayfishs
D004051 Diethylhexyl Phthalate An ester of phthalic acid. It appears as a light-colored, odorless liquid and is used as a plasticizer for many resins and elastomers. Dioctyl Phthalate,Bis(2-ethylhexyl)phthalate,DEHP,Di(2-ethylhexyl)phthalate,Di-2-Ethylhexylphthalate,Di 2 Ethylhexylphthalate,Phthalate, Diethylhexyl,Phthalate, Dioctyl
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D001559 Benzhydryl Compounds Compounds which contain the methyl radical substituted with two benzene rings. Permitted are any substituents, but ring fusion to any of the benzene rings is not allowed. Diphenylmethyl Compounds,Compounds, Benzhydryl,Compounds, Diphenylmethyl
D016923 Cell Death The termination of the cell's ability to carry out vital functions such as metabolism, growth, reproduction, responsiveness, and adaptability. Death, Cell
D052244 Endocrine Disruptors Exogenous agents, synthetic and naturally occurring, which are capable of disrupting the functions of the ENDOCRINE SYSTEM including the maintenance of HOMEOSTASIS and the regulation of developmental processes. Endocrine disruptors are compounds that can mimic HORMONES, or enhance or block the binding of hormones to their receptors, or otherwise lead to activating or inhibiting the endocrine signaling pathways and hormone metabolism. Endocrine Disrupting Chemical,Endocrine Disrupting Chemicals,Endocrine Disruptor,Endocrine Disruptor Effect,Endocrine Disruptor Effects,Chemical, Endocrine Disrupting,Chemicals, Endocrine Disrupting,Disrupting Chemical, Endocrine,Disruptor Effect, Endocrine,Disruptor Effects, Endocrine,Disruptor, Endocrine,Disruptors, Endocrine,Effect, Endocrine Disruptor,Effects, Endocrine Disruptor
D020128 Inhibitory Concentration 50 The concentration of a compound needed to reduce population growth of organisms, including eukaryotic cells, by 50% in vitro. Though often expressed to denote in vitro antibacterial activity, it is also used as a benchmark for cytotoxicity to eukaryotic cells in culture. IC50,Concentration 50, Inhibitory

Related Publications

B Yurdakok-Dikmen, and Y Turgut, and A Ç Gunal, and R Uyar, and O Kuzukıran, and A Filazi, and F Erkoc
May 2017, Fish & shellfish immunology,
B Yurdakok-Dikmen, and Y Turgut, and A Ç Gunal, and R Uyar, and O Kuzukıran, and A Filazi, and F Erkoc
October 2014, Genetica,
B Yurdakok-Dikmen, and Y Turgut, and A Ç Gunal, and R Uyar, and O Kuzukıran, and A Filazi, and F Erkoc
December 2016, Arhiv za higijenu rada i toksikologiju,
B Yurdakok-Dikmen, and Y Turgut, and A Ç Gunal, and R Uyar, and O Kuzukıran, and A Filazi, and F Erkoc
January 2019, Diseases of aquatic organisms,
B Yurdakok-Dikmen, and Y Turgut, and A Ç Gunal, and R Uyar, and O Kuzukıran, and A Filazi, and F Erkoc
July 2022, Environmental monitoring and assessment,
B Yurdakok-Dikmen, and Y Turgut, and A Ç Gunal, and R Uyar, and O Kuzukıran, and A Filazi, and F Erkoc
January 2011, Journal of biochemical and molecular toxicology,
B Yurdakok-Dikmen, and Y Turgut, and A Ç Gunal, and R Uyar, and O Kuzukıran, and A Filazi, and F Erkoc
July 2015, Environmental toxicology,
B Yurdakok-Dikmen, and Y Turgut, and A Ç Gunal, and R Uyar, and O Kuzukıran, and A Filazi, and F Erkoc
February 2022, Diseases of aquatic organisms,
B Yurdakok-Dikmen, and Y Turgut, and A Ç Gunal, and R Uyar, and O Kuzukıran, and A Filazi, and F Erkoc
January 1964, Tsitologiia,
B Yurdakok-Dikmen, and Y Turgut, and A Ç Gunal, and R Uyar, and O Kuzukıran, and A Filazi, and F Erkoc
January 2000, Physiological and biochemical zoology : PBZ,
Copied contents to your clipboard!