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The molecular cloning of a cDNA coding for human gastric lipase and its expression in yeast is described. A lipase present in human gastric aspirates was purified and its N-terminal amino-acid sequence was determined. This was found to be homologous with the N-terminal sequence of rat lingual lipase. A cDNA library was constructed from mRNA isolated from human stomach tissue and probed with cloned rat lingual lipase DNA. One clone, pGL17, consisting of approximately 1450 base-pairs, contained the entire coding sequence for a human gastric lipase. The amino-acid sequence from the isolated protein and the DNA sequence obtained from the cloned gene indicated that human gastric lipase consists of a 379 amino acid polypeptide with an unglycosylated Mr of 43,162. Human gastric lipase and rat lingual lipase amino-acid sequences were closely homologous but were unrelated to porcine pancreatic lipase apart from a 6 amino-acid sequence around the essential Ser-152 of porcine pancreatic lipase. A yeast expression plasmid containing the phosphoglycerate kinase promoter and terminator sequences together with the human gastric lipase gene was constructed. Yeast transformed with this vector synthesised the lipolytically active enzyme.
M W Bodmer, and
S Angal, and
G T Yarranton, and
T J Harris, and
A Lyons, and
D J King, and
G Pieroni, and
C Riviere, and
R Verger, and
P A Lowe
February 1988,
The Journal of biological chemistry,
M W Bodmer, and
S Angal, and
G T Yarranton, and
T J Harris, and
A Lyons, and
D J King, and
G Pieroni, and
C Riviere, and
R Verger, and
P A Lowe
March 2007,
FEMS yeast research,
M W Bodmer, and
S Angal, and
G T Yarranton, and
T J Harris, and
A Lyons, and
D J King, and
G Pieroni, and
C Riviere, and
R Verger, and
P A Lowe
February 1991,
Nucleic acids research,
M W Bodmer, and
S Angal, and
G T Yarranton, and
T J Harris, and
A Lyons, and
D J King, and
G Pieroni, and
C Riviere, and
R Verger, and
P A Lowe
November 1993,
FEBS letters,
M W Bodmer, and
S Angal, and
G T Yarranton, and
T J Harris, and
A Lyons, and
D J King, and
G Pieroni, and
C Riviere, and
R Verger, and
P A Lowe
April 1989,
Nature,
M W Bodmer, and
S Angal, and
G T Yarranton, and
T J Harris, and
A Lyons, and
D J King, and
G Pieroni, and
C Riviere, and
R Verger, and
P A Lowe
October 2004,
Journal of biotechnology,
M W Bodmer, and
S Angal, and
G T Yarranton, and
T J Harris, and
A Lyons, and
D J King, and
G Pieroni, and
C Riviere, and
R Verger, and
P A Lowe
April 1996,
The Journal of biological chemistry,
M W Bodmer, and
S Angal, and
G T Yarranton, and
T J Harris, and
A Lyons, and
D J King, and
G Pieroni, and
C Riviere, and
R Verger, and
P A Lowe
December 1993,
The Journal of biological chemistry,
M W Bodmer, and
S Angal, and
G T Yarranton, and
T J Harris, and
A Lyons, and
D J King, and
G Pieroni, and
C Riviere, and
R Verger, and
P A Lowe
August 1992,
Archives of biochemistry and biophysics,
M W Bodmer, and
S Angal, and
G T Yarranton, and
T J Harris, and
A Lyons, and
D J King, and
G Pieroni, and
C Riviere, and
R Verger, and
P A Lowe
November 1995,
Gene,
