Biomaterial Database

Comparison between proteinases of human seminal plasma and of sperm origin. 1987

M E Hume, and M S Siegel, and K L Polakoski

A comparison of the alkaline proteinase activity of human seminal plasma, the seminal non-gamete cellular material and spermatozoa was made by gelatin-sodium dodecyl sulfate-polyacrylamide gel electrophoresis (gelatin-SDS-PAGE) zymography. Several major (molecular weights = greater than 56,000) and minor (35,000 to 44,000) bands of proteinase activity were seen in the seminal plasma samples from nonvasectomized and vasectomized, healthy donors. Similar activity profiles were observed in the nongamete cellular material of vasectomized donor ejaculates. The major proteinase activity in sperm extracts was in the 47,000 to 55,000 (proacrosin-acrosin) and 34,000 to 37,000 (sperminogen-spermin) molecular weight ranges. These results suggest that the proacrosin-acrosin and sperminogen-spermin systems are of sperm origin and that there are considerable amounts of larger molecular weight trypsin-like enzymes in the soluble and nongamete cellular material of human seminal plasma.

UI	MeSH Term	Description	Entries
D008297	Male		Males
D008970	Molecular Weight	The sum of the weight of all the atoms in a molecule.	Molecular Weights,Weight, Molecular,Weights, Molecular
D010450	Endopeptidases	A subclass of PEPTIDE HYDROLASES that catalyze the internal cleavage of PEPTIDES or PROTEINS.	Endopeptidase,Peptide Peptidohydrolases
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D012661	Semen	The thick, yellowish-white, viscid fluid secretion of male reproductive organs discharged upon ejaculation. In addition to reproductive organ secretions, it contains SPERMATOZOA and their nutrient plasma.	Seminal Plasma,Plasma, Seminal
D013094	Spermatozoa	Mature male germ cells derived from SPERMATIDS. As spermatids move toward the lumen of the SEMINIFEROUS TUBULES, they undergo extensive structural changes including the loss of cytoplasm, condensation of CHROMATIN into the SPERM HEAD, formation of the ACROSOME cap, the SPERM MIDPIECE and the SPERM TAIL that provides motility.	Sperm,Spermatozoon,X-Bearing Sperm,X-Chromosome-Bearing Sperm,Y-Bearing Sperm,Y-Chromosome-Bearing Sperm,Sperm, X-Bearing,Sperm, X-Chromosome-Bearing,Sperm, Y-Bearing,Sperm, Y-Chromosome-Bearing,Sperms, X-Bearing,Sperms, X-Chromosome-Bearing,Sperms, Y-Bearing,Sperms, Y-Chromosome-Bearing,X Bearing Sperm,X Chromosome Bearing Sperm,X-Bearing Sperms,X-Chromosome-Bearing Sperms,Y Bearing Sperm,Y Chromosome Bearing Sperm,Y-Bearing Sperms,Y-Chromosome-Bearing Sperms
D014659	Vasectomy	Surgical removal of the ductus deferens, or a portion of it. It is done in association with prostatectomy, or to induce infertility. (Dorland, 28th ed)	Intravasal Thread,Vas Ligation,Vas Occlusion,Vas Occlusion, Nonchemical,Vasectomy Seekers,Vasectomy Seekers, Refused,Intravasal Threads,Ligation, Vas,Ligations, Vas,Nonchemical Vas Occlusion,Nonchemical Vas Occlusions,Occlusion, Nonchemical Vas,Occlusion, Vas,Occlusions, Nonchemical Vas,Occlusions, Vas,Refused Vasectomy Seeker,Refused Vasectomy Seekers,Seeker, Refused Vasectomy,Seeker, Vasectomy,Seekers, Refused Vasectomy,Seekers, Vasectomy,Thread, Intravasal,Threads, Intravasal,Vas Ligations,Vas Occlusions,Vas Occlusions, Nonchemical,Vasectomies,Vasectomy Seeker,Vasectomy Seeker, Refused