BIOMAT Database Logo BIOMAT Database Logo

[Cloning of the regulator gene of Erwinia carotovora repressing pectate lyase ptlA gene expression]. 1987

N O Bukanov, and M Iu Fonshteĭn, and P Dobrovol'ski, and N K Iankovskiĭ, and V G Debabov

Pectate lyase synthesis in the cells of Erwinia carotovora ELA 49 is induced by polypectate. This suggested that the Erwinia chromosomes carried a regulator gene responsible for negative regulation of the pectate lyase gene expression. In the present study the regulator gene controlling expression of one of the pectate lyase structural genes was cloned and designated as ptlA gene. For this purpose a genetic system with the tester plasmid pPc624 as the main element was constructed. The tester plasmid contained cat gene (resistance to chloramphenicol) controlled by the promotor of the ptlA gene cloned on vector pPD620. Plasmid pPC624 was maintained in the E. coli cells in a number of 1-2 copies and transferred resistance to chloramphenicol in concentrations up to 100 micrograms/ml to the cells. The E. carotovora cells containing pPC624 were sensitive to chloramphenicol in media containing no inductor (sodium polypectate). In media with the inductor they were resistant to chloramphenicol. Therefore, plasmid pPC624 proved to be a suitable system for testing the regulator gene product. The E. coli cells containing plasmid pPC624 were transformed by the hybrid Ptl+ plasmids identified in the clonotheque of the Erwinia DNA EcoRI fragments. The E. coli cotransformants were characterized by chloramphenicol sensitivity which provided a conclusion that the regulator ptlR gene controlling the ptlA gene expression was localized on the DNA EcoRI fragment (7.3 kb) containing the pectate lyase ptlA and ptlB genes. Deletion analysis showed that the investigated genes were localized in the EcoRI fragment (7.3 kb) of the E. carotovora chromosomal DNA in the following order: ptlA--ptlB--ptlR.(ABSTRACT TRUNCATED AT 250 WORDS)

UI MeSH Term Description Entries
D010957 Plasmids Extrachromosomal, usually CIRCULAR DNA molecules that are self-replicating and transferable from one organism to another. They are found in a variety of bacterial, archaeal, fungal, algal, and plant species. They are used in GENETIC ENGINEERING as CLONING VECTORS. Episomes,Episome,Plasmid
D011133 Polysaccharide-Lyases A group of carbon-oxygen lyases. These enzymes catalyze the breakage of a carbon-oxygen bond in polysaccharides leading to an unsaturated product and the elimination of an alcohol. EC 4.2.2. Polysaccharide Lyase,Polysaccharide-Lyase,Lyase, Polysaccharide,Polysaccharide Lyases
D002701 Chloramphenicol An antibiotic first isolated from cultures of Streptomyces venequelae in 1947 but now produced synthetically. It has a relatively simple structure and was the first broad-spectrum antibiotic to be discovered. It acts by interfering with bacterial protein synthesis and is mainly bacteriostatic. (From Martindale, The Extra Pharmacopoeia, 29th ed, p106) Cloranfenicol,Kloramfenikol,Levomycetin,Amphenicol,Amphenicols,Chlornitromycin,Chlorocid,Chloromycetin,Detreomycin,Ophthochlor,Syntomycin
D003001 Cloning, Molecular The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells. Molecular Cloning
D004269 DNA, Bacterial Deoxyribonucleic acid that makes up the genetic material of bacteria. Bacterial DNA
D004352 Drug Resistance, Microbial The ability of microorganisms, especially bacteria, to resist or to become tolerant to chemotherapeutic agents, antimicrobial agents, or antibiotics. This resistance may be acquired through gene mutation or foreign DNA in transmissible plasmids (R FACTORS). Antibiotic Resistance,Antibiotic Resistance, Microbial,Antimicrobial Resistance, Drug,Antimicrobial Drug Resistance,Antimicrobial Drug Resistances,Antimicrobial Resistances, Drug,Drug Antimicrobial Resistance,Drug Antimicrobial Resistances,Drug Resistances, Microbial,Resistance, Antibiotic,Resistance, Drug Antimicrobial,Resistances, Drug Antimicrobial
D004885 Erwinia A genus of gram-negative, facultatively anaerobic, rod-shaped bacteria whose organisms are associated with plants as pathogens, saprophytes, or as constituents of the epiphytic flora.
D004926 Escherichia coli A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc. Alkalescens-Dispar Group,Bacillus coli,Bacterium coli,Bacterium coli commune,Diffusely Adherent Escherichia coli,E coli,EAggEC,Enteroaggregative Escherichia coli,Enterococcus coli,Diffusely Adherent E. coli,Enteroaggregative E. coli,Enteroinvasive E. coli,Enteroinvasive Escherichia coli
D005786 Gene Expression Regulation Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control (induction or repression) of gene action at the level of transcription or translation. Gene Action Regulation,Regulation of Gene Expression,Expression Regulation, Gene,Regulation, Gene Action,Regulation, Gene Expression
D005798 Genes, Bacterial The functional hereditary units of BACTERIA. Bacterial Gene,Bacterial Genes,Gene, Bacterial

Related Publications

N O Bukanov, and M Iu Fonshteĭn, and P Dobrovol'ski, and N K Iankovskiĭ, and V G Debabov
Cloning and characterization of a pectate lyase gene from Erwinia carotovora EC153.
January 1989, Molecular plant-microbe interactions : MPMI,
N O Bukanov, and M Iu Fonshteĭn, and P Dobrovol'ski, and N K Iankovskiĭ, and V G Debabov
Cloning and characterization of the pectate lyase III gene of Erwinia carotovora Er.
April 1991, Agricultural and biological chemistry,
N O Bukanov, and M Iu Fonshteĭn, and P Dobrovol'ski, and N K Iankovskiĭ, and V G Debabov
Molecular cloning and sequencing of the extracellular pectate lyase II gene from Erwinia carotovora Er.
October 1992, Bioscience, biotechnology, and biochemistry,
N O Bukanov, and M Iu Fonshteĭn, and P Dobrovol'ski, and N K Iankovskiĭ, and V G Debabov
Cloning of the pectate lyase genes from Erwinia carotovora and their expression in Escherichia coli.
January 1985, Gene,
N O Bukanov, and M Iu Fonshteĭn, and P Dobrovol'ski, and N K Iankovskiĭ, and V G Debabov
Characterization of a novel pectate lyase from Erwinia carotovora subsp. carotovora.
January 1995, Molecular plant-microbe interactions : MPMI,
N O Bukanov, and M Iu Fonshteĭn, and P Dobrovol'ski, and N K Iankovskiĭ, and V G Debabov
Characterization of the Erwinia carotovora pelB gene and its product pectate lyase.
September 1987, Journal of bacteriology,
N O Bukanov, and M Iu Fonshteĭn, and P Dobrovol'ski, and N K Iankovskiĭ, and V G Debabov
"Self-catabolite repression" of pectate lyase in Erwinia carotovora.
February 1979, Journal of bacteriology,
N O Bukanov, and M Iu Fonshteĭn, and P Dobrovol'ski, and N K Iankovskiĭ, and V G Debabov
Characterization of the Erwinia carotovora pelA gene and its product pectate lyase A.
January 1988, Gene,
N O Bukanov, and M Iu Fonshteĭn, and P Dobrovol'ski, and N K Iankovskiĭ, and V G Debabov
[Expression of pectate lyase genes of Erwinia carotovora subsp. carotovora 17A and Erwinia carotovora subsp. atroseptica 36A in Erwinia carotovor substp. atroseptica 36A cells].
January 1994, Molekuliarnaia genetika, mikrobiologiia i virusologiia,
N O Bukanov, and M Iu Fonshteĭn, and P Dobrovol'ski, and N K Iankovskiĭ, and V G Debabov
Cloning and expression of pectin lyase gene from Erwinia carotovora in Escherichia coli.
April 1990, Biochemical and biophysical research communications,
Copied contents to your clipboard!