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Decidual prolactin-releasing factor (PRL-RF), a placental protein that stimulates the release of prolactin from human decidual tissue, has been purified from conditioned medium of human placental explants. The purification scheme consisted of ethanol extraction, anion exchange chromatography on DEAE-cellulose, size exclusion chromatography on Spherogel TSK-3000, and either a) immunoaffinity chromatography using an antiserum to a partially purified PRL-RF preparation or b) acetic acid-urea/SDS 2-dimensional PAGE. The apparent molecular weight of the purified releasing factor, estimated by SDS-PAGE, was 23,500 Da; and the half-maximal dose for the acute stimulation of prolactin release from human decidual cells was 0.05-0.1 ug/ml (2.2-4.4 nM).
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D Capel, and
G Korner, and
R Richards
January 1992,
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S Handwerger, and
D Capel, and
G Korner, and
R Richards
June 1989,
Endocrinology,
S Handwerger, and
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G Korner, and
R Richards
December 1988,
Endocrinology,
S Handwerger, and
D Capel, and
G Korner, and
R Richards
October 1988,
Endocrinology,
S Handwerger, and
D Capel, and
G Korner, and
R Richards
January 1979,
Neuroendocrinology,
S Handwerger, and
D Capel, and
G Korner, and
R Richards
December 1987,
Clinical endocrinology,
S Handwerger, and
D Capel, and
G Korner, and
R Richards
January 1991,
Annals of the New York Academy of Sciences,
S Handwerger, and
D Capel, and
G Korner, and
R Richards
January 1994,
Biology of reproduction,
S Handwerger, and
D Capel, and
G Korner, and
R Richards
April 1982,
The Journal of clinical endocrinology and metabolism,
S Handwerger, and
D Capel, and
G Korner, and
R Richards
April 1983,
Endocrinology,
