-transparent.png){kind=logo}
A rabbit monospecific antibody for mouse beta-spectrin was used to screen a mouse anemic spleen cDNA expression library. A mouse beta-spectrin cDNA clone was isolated and identified by its ability to make mouse beta-spectrin-like antigens in Escherichia coli. This clone was used to probe total RNA from various mouse tissues. Anemic spleen RNA showed two strongly hybridizing RNA species of approximately 6 and 8 kb. Two very faintly hybridizing bands of about 6 kb and 10 kb could also be seen in total mouse brain RNA. All of these bands could be detected after hybridization under both stringent and nonstringent conditions. This suggests that erythroid beta-spectrin may also be expressed in the brain. No bands could be detected in kidney, liver, or spleen RNA. Southern blot analysis of mouse genomic DNA showed a single hybridizing band after digestion with several restriction endonucleases even under nonstringent conditions. Nucleotide sequencing of the cDNA insert revealed almost complete identity between the N-terminus of the deduced amino acid sequence of the cDNA clone and the C-terminal 15 amino acids of a peptide derived from the beta-8 repeat unit of human erythrocyte beta-spectrin. The deduced amino acid sequence contained most of the conserved amino acids characteristic of the 106 amino acid repeat unit first found in human alpha-spectrin and thus provides the first evidence for a complete 106 amino acid repeat unit structure in beta-spectrin.
L Cioè, and
P Laurila, and
P Meo, and
K Krebs, and
S Goodman, and
P J Curtis
July 1988,
Blood,
L Cioè, and
P Laurila, and
P Meo, and
K Krebs, and
S Goodman, and
P J Curtis
February 1993,
Biochimica et biophysica acta,
L Cioè, and
P Laurila, and
P Meo, and
K Krebs, and
S Goodman, and
P J Curtis
October 1986,
Nucleic acids research,
L Cioè, and
P Laurila, and
P Meo, and
K Krebs, and
S Goodman, and
P J Curtis
December 1982,
Proceedings of the National Academy of Sciences of the United States of America,
L Cioè, and
P Laurila, and
P Meo, and
K Krebs, and
S Goodman, and
P J Curtis
February 1994,
Biochemical and biophysical research communications,
L Cioè, and
P Laurila, and
P Meo, and
K Krebs, and
S Goodman, and
P J Curtis
November 1992,
Biochimica et biophysica acta,
L Cioè, and
P Laurila, and
P Meo, and
K Krebs, and
S Goodman, and
P J Curtis
January 1985,
Gene,
L Cioè, and
P Laurila, and
P Meo, and
K Krebs, and
S Goodman, and
P J Curtis
January 1989,
FEBS letters,
L Cioè, and
P Laurila, and
P Meo, and
K Krebs, and
S Goodman, and
P J Curtis
August 1991,
Biochimica et biophysica acta,
L Cioè, and
P Laurila, and
P Meo, and
K Krebs, and
S Goodman, and
P J Curtis
November 1997,
Biochemical pharmacology,
