Biomaterial Database

Novel genetic components controlling invertase production in Saccharomyces cerevisiae. 1987

L del Castillo Agudo, and F K Zimmermann

Institut für Mikrobiologie, Darmstadt, FRG.

Low levels of invertase (EC 3.2.1.26) activity were observed in most diploid strains of S. cerevisiae used in this work. There was no effect of mating type on invertase levels, and cell surface was not a limiting factor, because an increase in ploidy did not cause further decrease in specific invertase activity. Finally, some diploids showed the activity expected from the additive effects of different SUC genes, and haploid strains possessing two SUC genes expressed very variable invertase activities depending on the strain. This suggested the existence of one or more additional genes which control the levels of invertase. Genetic analysis of SUC5 strains provided evidence of the existence of a new gene, RPS5, which drastically reduced the specific invertase activity in strains possessing active SUC alleles. The recessive allele of this gene (rps5) allows expression of higher levels of invertase. We suggest that genes similar RPS5 are responsible for the low levels of invertase activity observed in diploid strains of S. cerevisiae.

UI	MeSH Term	Description	Entries
D004171	Diploidy	The chromosomal constitution of cells, in which each type of CHROMOSOME is represented twice. Symbol: 2N or 2X.	Diploid,Diploid Cell,Cell, Diploid,Cells, Diploid,Diploid Cells,Diploidies,Diploids
D005786	Gene Expression Regulation	Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control (induction or repression) of gene action at the level of transcription or translation.	Gene Action Regulation,Regulation of Gene Expression,Expression Regulation, Gene,Regulation, Gene Action,Regulation, Gene Expression
D005800	Genes, Fungal	The functional hereditary units of FUNGI.	Fungal Genes,Fungal Gene,Gene, Fungal
D006026	Glycoside Hydrolases	Any member of the class of enzymes that catalyze the cleavage of the glycosidic linkage of glycosides and the addition of water to the resulting molecules.	Endoglycosidase,Exoglycosidase,Glycohydrolase,Glycosidase,Glycosidases,Glycoside Hydrolase,Endoglycosidases,Exoglycosidases,Glycohydrolases,Hydrolase, Glycoside,Hydrolases, Glycoside
D006238	Haploidy	The chromosomal constitution of cells, in which each type of CHROMOSOME is represented once. Symbol: N.	Haploid,Haploid Cell,Cell, Haploid,Cells, Haploid,Haploid Cells,Haploidies,Haploids
D012441	Saccharomyces cerevisiae	A species of the genus SACCHAROMYCES, family Saccharomycetaceae, order Saccharomycetales, known as "baker's" or "brewer's" yeast. The dried form is used as a dietary supplement.	Baker's Yeast,Brewer's Yeast,Candida robusta,S. cerevisiae,Saccharomyces capensis,Saccharomyces italicus,Saccharomyces oviformis,Saccharomyces uvarum var. melibiosus,Yeast, Baker's,Yeast, Brewer's,Baker Yeast,S cerevisiae,Baker's Yeasts,Yeast, Baker
D043324	beta-Fructofuranosidase	A glycoside hydrolase found primarily in PLANTS and YEASTS. It has specificity for beta-D-fructofuranosides such as SUCROSE.	beta-D-Fructofuranosidase,Acid Invertase,Invertase,Saccharase,Sacrosidase,Sucraid,Sucrose 6-Phosphate Hydrolase,beta-Fructosidase,6-Phosphate Hydrolase, Sucrose,Hydrolase, Sucrose 6-Phosphate,Invertase, Acid,Sucrose 6 Phosphate Hydrolase,beta D Fructofuranosidase,beta Fructofuranosidase,beta Fructosidase
D049770	Genes, Mating Type, Fungal	Fungal genes that mostly encode TRANSCRIPTION FACTORS. In some FUNGI they also encode PHEROMONES and PHEROMONE RECEPTORS. The transcription factors control expression of specific proteins that give a cell its mating identity. Opposite mating type identities are required for mating.	Fungal Mating-Type Genes,Genes, Fungal Mating Type,Mating Type, Fungal,Fungal Mating Type,Fungal Mating Type Genes,Fungal Mating Types,Fungal Mating-Type Gene,Gene, Fungal Mating-Type,Genes, Fungal Mating-Type,Mating Types, Fungal,Mating-Type Gene, Fungal,Mating-Type Genes, Fungal,Type, Fungal Mating,Types, Fungal Mating