Preservation of connexin 43 and transzonal projections in isolated bovine pre-antral follicles before and following vitrification. 2021

Anniek Bus, and Katarzyna Szymanska, and Isabel Pintelon, and Jo L M R Leroy, and Luc Leybaert, and Peter E J Bols
Faculty of Pharmaceutical, Biomedical and Veterinary Sciences, Laboratory of Veterinary Physiology and Biochemistry, Gamete Research Centre, University of Antwerp, Universiteitsplein 1, U building, 2610, Wilrijk, Belgium. anniek.bus@uantwerp.be.

OBJECTIVE Gap junctions and transzonal projections play a crucial role in intercellular communication between different follicular components and are necessary for follicle development. We aimed to demonstrate gap junction protein connexin 43 (Cx43) and transzonal projections (TZPs) in viable, category 1, isolated bovine pre-antral follicles (PAFs) during short-term culture and after vitrification and warming. METHODS This study involved four experimental groups: fresh control, 2-day culture, 4-day culture, and vitrified secondary PAFs. Isolated PAFs were vitrified using a simple and efficient cryopreservation method by means of mini cell strainers. RESULTS Cx43 and TZPs were detected in pre-antral follicles of all stages, as well as in every experimental group. The group fresh follicles showed a higher percentage of follicles that were positive for Cx43 (91.7%) than the follicles that were vitrified (77.4%). All follicles that were cultured for 2 days were Cx43-positive (100%). Follicles cultured for 4 days (65.8%) (P = 0.002) showed the lowest percentage of follicles that were Cx43-positive. The percentages of the presence or (partial) absence of the TZP network were shown to be very heterogeneous between follicles in different treatment groups. CONCLUSIONS These results suggest the maintenance of communication between the oocyte and the somatic companion cells after vitrification and warming. The varying percentages of the expression of the TZP network within groups suggests that it will be of interest to investigate whether this is truly due to variability in TZP integrity and follicle quality or due to methodological limitations.

UI MeSH Term Description Entries
D009865 Oocytes Female germ cells derived from OOGONIA and termed OOCYTES when they enter MEIOSIS. The primary oocytes begin meiosis but are arrested at the diplotene state until OVULATION at PUBERTY to give rise to haploid secondary oocytes or ova (OVUM). Ovocytes,Oocyte,Ovocyte
D002417 Cattle Domesticated bovine animals of the genus Bos, usually kept on a farm or ranch and used for the production of meat or dairy products or for heavy labor. Beef Cow,Bos grunniens,Bos indicus,Bos indicus Cattle,Bos taurus,Cow,Cow, Domestic,Dairy Cow,Holstein Cow,Indicine Cattle,Taurine Cattle,Taurus Cattle,Yak,Zebu,Beef Cows,Bos indicus Cattles,Cattle, Bos indicus,Cattle, Indicine,Cattle, Taurine,Cattle, Taurus,Cattles, Bos indicus,Cattles, Indicine,Cattles, Taurine,Cattles, Taurus,Cow, Beef,Cow, Dairy,Cow, Holstein,Cows,Dairy Cows,Domestic Cow,Domestic Cows,Indicine Cattles,Taurine Cattles,Taurus Cattles,Yaks,Zebus
D005260 Female Females
D006080 Ovarian Follicle An OOCYTE-containing structure in the cortex of the OVARY. The oocyte is enclosed by a layer of GRANULOSA CELLS providing a nourishing microenvironment (FOLLICULAR FLUID). The number and size of follicles vary depending on the age and reproductive state of the female. The growing follicles are divided into five stages: primary, secondary, tertiary, Graafian, and atretic. Follicular growth and steroidogenesis depend on the presence of GONADOTROPINS. Graafian Follicle,Atretic Follicle,Ovarian Follicles,Atretic Follicles,Follicle, Atretic,Follicle, Graafian,Follicle, Ovarian,Follicles, Atretic,Follicles, Graafian,Follicles, Ovarian,Graafian Follicles
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D015571 Follicular Fluid The fluid surrounding the OVUM and GRANULOSA CELLS in the Graafian follicle (OVARIAN FOLLICLE). The follicular fluid contains sex steroids, glycoprotein hormones, plasma proteins, mucopolysaccharides, and enzymes. Antral Fluid, Ovarian Follicle,Liquor Folliculi,Fluid, Follicular,Follicular Fluids,Folliculi, Liquor
D015925 Cryopreservation Preservation of cells, tissues, organs, or embryos by freezing. In histological preparations, cryopreservation or cryofixation is used to maintain the existing form, structure, and chemical composition of all the constituent elements of the specimens. Cryofixation,Cryonic Suspension,Cryonic Suspensions,Suspension, Cryonic
D017629 Gap Junctions Connections between cells which allow passage of small molecules and electric current. Gap junctions were first described anatomically as regions of close apposition between cells with a narrow (1-2 nm) gap between cell membranes. The variety in the properties of gap junctions is reflected in the number of CONNEXINS, the family of proteins which form the junctions. Gap Junction,Junction, Gap,Junctions, Gap
D058989 Vitrification The transformation of a liquid to a glassy solid i.e., without the formation of crystals during the cooling process. Glass Transition,Glass-Liquid Transition,Liquid-Glass Transition,Glass Liquid Transition,Liquid Glass Transition,Transition, Glass,Transition, Glass-Liquid,Transition, Liquid-Glass
D018031 Connexin 43 A 43-kDa peptide which is a member of the connexin family of gap junction proteins. Connexin 43 is a product of a gene in the alpha class of connexin genes (the alpha-1 gene). It was first isolated from mammalian heart, but is widespread in the body including the brain. Cx43,Connexin43

Related Publications

Anniek Bus, and Katarzyna Szymanska, and Isabel Pintelon, and Jo L M R Leroy, and Luc Leybaert, and Peter E J Bols
July 2018, Journal of assisted reproduction and genetics,
Anniek Bus, and Katarzyna Szymanska, and Isabel Pintelon, and Jo L M R Leroy, and Luc Leybaert, and Peter E J Bols
October 2013, Reproduction in domestic animals = Zuchthygiene,
Anniek Bus, and Katarzyna Szymanska, and Isabel Pintelon, and Jo L M R Leroy, and Luc Leybaert, and Peter E J Bols
May 2013, Human reproduction (Oxford, England),
Anniek Bus, and Katarzyna Szymanska, and Isabel Pintelon, and Jo L M R Leroy, and Luc Leybaert, and Peter E J Bols
October 2021, The Journal of reproduction and development,
Anniek Bus, and Katarzyna Szymanska, and Isabel Pintelon, and Jo L M R Leroy, and Luc Leybaert, and Peter E J Bols
May 2016, Theriogenology,
Anniek Bus, and Katarzyna Szymanska, and Isabel Pintelon, and Jo L M R Leroy, and Luc Leybaert, and Peter E J Bols
September 2010, Molecular human reproduction,
Anniek Bus, and Katarzyna Szymanska, and Isabel Pintelon, and Jo L M R Leroy, and Luc Leybaert, and Peter E J Bols
December 2007, Heart failure reviews,
Anniek Bus, and Katarzyna Szymanska, and Isabel Pintelon, and Jo L M R Leroy, and Luc Leybaert, and Peter E J Bols
May 2006, Molecular reproduction and development,
Anniek Bus, and Katarzyna Szymanska, and Isabel Pintelon, and Jo L M R Leroy, and Luc Leybaert, and Peter E J Bols
May 1990, Journal of animal science,
Anniek Bus, and Katarzyna Szymanska, and Isabel Pintelon, and Jo L M R Leroy, and Luc Leybaert, and Peter E J Bols
January 2003, Human reproduction update,
Copied contents to your clipboard!