BIOMAT Database Logo BIOMAT Database Logo

Improved dual luciferase reporter (DLR) assay to determine the protein stability. 2021

Yinglu Sun, and Jinfeng Zhao, and Suyash Bhimgonda Patil, and Jingjing Fang, and Jun Liu, and Xueyong Li
National Key Facility for Crop Gene Resources and Genetic Improvement, ICS, CAAS, 12 Zhongguancun South Street, Haidian District, Beijing, 100081, China. Electronic address: 339058452@qq.com.

We developed a binary vector co-expressing firefly luciferase (FF) and Renilla luciferase (REN) to detect protein stability in response to different stimuli, and verified the functionality of the vector. The StrigoQuant-like reporter expressing FF and REN in one transcript is a sensitive tool for detecting protein abundance in different genotypes. However, we found that significant differences in the relative FF/REN ratio of empty StrigoQuant vector in different genotypes. Therefore, to determine the actual protein abundance, the relative FF/REN ratio of the protein of interest should be normalized to that of the empty vector.

UI MeSH Term Description Entries
D007202 Indicators and Reagents Substances used for the detection, identification, analysis, etc. of chemical, biological, or pathologic processes or conditions. Indicators are substances that change in physical appearance, e.g., color, at or approaching the endpoint of a chemical titration, e.g., on the passage between acidity and alkalinity. Reagents are substances used for the detection or determination of another substance by chemical or microscopical means, especially analysis. Types of reagents are precipitants, solvents, oxidizers, reducers, fluxes, and colorimetric reagents. (From Grant & Hackh's Chemical Dictionary, 5th ed, p301, p499) Indicator,Reagent,Reagents,Indicators,Reagents and Indicators
D007783 Lactones Cyclic esters of hydroxy carboxylic acids, containing a 1-oxacycloalkan-2-one structure. Large cyclic lactones of over a dozen atoms are MACROLIDES. Lactone
D008156 Luciferases Enzymes that oxidize certain LUMINESCENT AGENTS to emit light (PHYSICAL LUMINESCENCE). The luciferases from different organisms have evolved differently so have different structures and substrates. Luciferase
D010937 Plant Growth Regulators Any of the hormones produced naturally in plants and active in controlling growth and other functions. There are three primary classes: auxins, cytokinins, and gibberellins. Phytohormone,Phytohormones,Plant Growth Regulator,Plant Hormone,Plant Hormones,Growth Regulators, Plant,Regulators, Plant Growth,Growth Regulator, Plant,Hormone, Plant,Hormones, Plant,Regulator, Plant Growth
D011523 Protoplasts The protoplasm and plasma membrane of plant, fungal, bacterial or archaeon cells without the CELL WALL. Protoplast
D005822 Genetic Vectors DNA molecules capable of autonomous replication within a host cell and into which other DNA sequences can be inserted and thus amplified. Many are derived from PLASMIDS; BACTERIOPHAGES; or VIRUSES. They are used for transporting foreign genes into recipient cells. Genetic vectors possess a functional replicator site and contain GENETIC MARKERS to facilitate their selective recognition. Cloning Vectors,Shuttle Vectors,Vectors, Genetic,Cloning Vector,Genetic Vector,Shuttle Vector,Vector, Cloning,Vector, Genetic,Vector, Shuttle,Vectors, Cloning,Vectors, Shuttle
D006575 Heterocyclic Compounds, 3-Ring A class of heterocyclic compounds that include a three-ring fused structure. Both aromatic or non-aromatic ring structures are included in this category. Fused Heterocyclic Compounds, Three-Ring,Heterocyclic Cpds, 3 Ring,Three Ring Heterocyclic Compounds,3-Ring Heterocyclic Compounds,Fused Heterocyclic Compounds, Three Ring,Heterocyclic Compounds, 3 Ring
D001681 Biological Assay A method of measuring the effects of a biologically active substance using an intermediate in vivo or in vitro tissue or cell model under controlled conditions. It includes virulence studies in animal fetuses in utero, mouse convulsion bioassay of insulin, quantitation of tumor-initiator systems in mouse skin, calculation of potentiating effects of a hormonal factor in an isolated strip of contracting stomach muscle, etc. Bioassay,Assay, Biological,Assays, Biological,Biologic Assay,Biologic Assays,Assay, Biologic,Assays, Biologic,Bioassays,Biological Assays
D012275 Oryza A genus of grass family (Poaceae) that include several rice species. Oryza sativa,Rice,Rices
D014026 Nicotiana A plant genus of the family SOLANACEAE. Members contain NICOTINE and other biologically active chemicals; the dried leaves of Nicotiana tabacum are used for SMOKING. Tobacco Plant,Nicotiana tabacum,Plant, Tobacco,Plants, Tobacco,Tobacco Plants

Related Publications

Yinglu Sun, and Jinfeng Zhao, and Suyash Bhimgonda Patil, and Jingjing Fang, and Jun Liu, and Xueyong Li
Improved dual-luciferase reporter assays for nuclear receptors.
January 2010, Current chemical genomics,
Yinglu Sun, and Jinfeng Zhao, and Suyash Bhimgonda Patil, and Jingjing Fang, and Jun Liu, and Xueyong Li
Dual-reporter assay using two secreted luciferase genes.
March 2007, BioTechniques,
Yinglu Sun, and Jinfeng Zhao, and Suyash Bhimgonda Patil, and Jingjing Fang, and Jun Liu, and Xueyong Li
Secreted dual reporter assay with Gaussia luciferase and the red fluorescent protein mCherry.
January 2017, PloS one,
Yinglu Sun, and Jinfeng Zhao, and Suyash Bhimgonda Patil, and Jingjing Fang, and Jun Liu, and Xueyong Li
Luciferase reporter assay.
January 2006, Sub-cellular biochemistry,
Yinglu Sun, and Jinfeng Zhao, and Suyash Bhimgonda Patil, and Jingjing Fang, and Jun Liu, and Xueyong Li
Relative quantification of protein-protein interactions using a dual luciferase reporter pull-down assay system.
January 2011, PloS one,
Yinglu Sun, and Jinfeng Zhao, and Suyash Bhimgonda Patil, and Jingjing Fang, and Jun Liu, and Xueyong Li
A luciferase reporter assay to investigate the differential selenium-dependent stability of selenoprotein mRNAs.
October 2012, The Journal of nutritional biochemistry,
Yinglu Sun, and Jinfeng Zhao, and Suyash Bhimgonda Patil, and Jingjing Fang, and Jun Liu, and Xueyong Li
A noncommercial dual luciferase enzyme assay system for reporter gene analysis.
June 2000, Analytical biochemistry,
Yinglu Sun, and Jinfeng Zhao, and Suyash Bhimgonda Patil, and Jingjing Fang, and Jun Liu, and Xueyong Li
Dual luminescence-based reporter gene assay for luciferase and beta-galactosidase.
September 1996, BioTechniques,
Yinglu Sun, and Jinfeng Zhao, and Suyash Bhimgonda Patil, and Jingjing Fang, and Jun Liu, and Xueyong Li
Detection of E2F-Induced Transcriptional Activity Using a Dual Luciferase Reporter Assay.
January 2018, Methods in molecular biology (Clifton, N.J.),
Yinglu Sun, and Jinfeng Zhao, and Suyash Bhimgonda Patil, and Jingjing Fang, and Jun Liu, and Xueyong Li
Application of the dual-luciferase reporter assay to the analysis of promoter activity in Zebrafish embryos.
October 2008, BMC biotechnology,
Copied contents to your clipboard!