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To determine the effect of insulin on its receptor concentrations in hepatocytes of fetal and adult rats, these cells were preincubated in the presence or absence of insulin. The reduced [125I]-insulin binding observed in adult hepatocytes was dependent on the concentration of insulin and on the duration of exposure, while in fetal hepatocytes insulin did not induce any reduction in insulin binding. In contrast, glucagon receptors were unaffected by preincubation with insulin. The modifications observed in insulin binding were accounted for by changes in receptor concentrations rather than any change in receptor affinity for the hormone. Studies on the kinetic properties of the insulin receptors of fetuses and adult rats revealed that association and dissociation rates were undistinguishable. These results indicate an absence of insulin receptor down-regulation in the fetus, which could favour anabolic processes during intrauterine life.
E Alvarez, and
E Blázquez
January 1991,
Cell biochemistry and function,
E Alvarez, and
E Blázquez
December 1991,
Diabetologia,
E Alvarez, and
E Blázquez
October 1979,
Biochemical Society transactions,
E Alvarez, and
E Blázquez
December 1978,
Endocrinology,
E Alvarez, and
E Blázquez
September 1986,
Biochimica et biophysica acta,
E Alvarez, and
E Blázquez
December 1984,
The American journal of physiology,
E Alvarez, and
E Blázquez
August 1986,
Endocrinology,
E Alvarez, and
E Blázquez
December 1987,
Endocrinology,
E Alvarez, and
E Blázquez
December 1989,
Biochemical medicine and metabolic biology,
E Alvarez, and
E Blázquez
September 1988,
In vitro cellular & developmental biology : journal of the Tissue Culture Association,
