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The convenience of the previously described enzyme immunochromatography method for visually quantifying theophylline in whole blood has been improved with the development of a one-step protocol. The capillary migration and color generation in the two-step enzyme immunochromatographic assay have been combined into a single step. Ascorbic acid is used as a signal inhibitor to delay enzymatic color product formation until the inhibitor itself is consumed. The concept of internal delay reaction is presented and the mechanism of ascorbate's action as an inhibitor to temporarily delay color generation is described. The internal delay reaction has been applied to a practical one-step quantitative visual enzyme immunochromatographic assay for theophylline in whole blood.
T M Li, and
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S N Stiso, and
N C Sizto, and
R F Zuk, and
D J Litman
September 2000,
Methods (San Diego, Calif.),
T M Li, and
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March 2002,
Lancet (London, England),
T M Li, and
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January 2010,
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Analytical and bioanalytical chemistry,
T M Li, and
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April 2012,
Journal of natural medicines,
T M Li, and
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January 2019,
Journal of animal science and biotechnology,
T M Li, and
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D J Litman
April 2004,
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T M Li, and
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June 2009,
Journal of virological methods,
T M Li, and
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S N Stiso, and
N C Sizto, and
R F Zuk, and
D J Litman
April 1993,
Clinical chemistry,
T M Li, and
R Chen, and
S Leeder, and
S N Stiso, and
N C Sizto, and
R F Zuk, and
D J Litman
April 2010,
Journal of the science of food and agriculture,
